ID   EME1_ORYSJ              Reviewed;         650 AA.
AC   Q0J9J6; A0A0P0WFK5; Q7XT54;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Crossover junction endonuclease EME1;
DE            EC=3.1.22.-;
DE   AltName: Full=Essential meiotic endonuclease 1;
DE            Short=OsEME1;
GN   Name=EME1; OrderedLocusNames=Os04g0648700, LOC_Os04g55500;
GN   ORFNames=OsJ_16416, OSJNBa0010D21.8;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Interacts with MUS81 to form a DNA structure-specific
CC       endonuclease with substrate preference for branched DNA structures with
CC       a 5'-end at the branch nick. Typical substrates include 3'-flap
CC       structures, D-loops, replication forks, nicked Holliday junctions and
CC       also intact Holliday junctions with a reduced efficiency. May be
CC       required in mitosis for the processing of stalled or collapsed
CC       replication fork intermediates. Plays a role in DNA repair and in
CC       genotoxic stress-induced homologous recombination (HR) in somatic
CC       cells. Mediates a subset of meiotic recombination events that are
CC       insensitive to crossover interference (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Forms a heterodimer with MUS81. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EME1/MMS4 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE01666.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL606635; CAE01666.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008210; BAF15991.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS91328.1; -; Genomic_DNA.
DR   EMBL; CM000141; EEE61803.1; -; Genomic_DNA.
DR   EMBL; AK066786; BAG90127.1; -; mRNA.
DR   RefSeq; XP_015637261.1; XM_015781775.1.
DR   AlphaFoldDB; Q0J9J6; -.
DR   SMR; Q0J9J6; -.
DR   STRING; 4530.OS04T0648700-01; -.
DR   iPTMnet; Q0J9J6; -.
DR   PaxDb; Q0J9J6; -.
DR   PRIDE; Q0J9J6; -.
DR   EnsemblPlants; Os04t0648700-01; Os04t0648700-01; Os04g0648700.
DR   GeneID; 4337213; -.
DR   Gramene; Os04t0648700-01; Os04t0648700-01; Os04g0648700.
DR   KEGG; osa:4337213; -.
DR   eggNOG; ENOG502QV6A; Eukaryota.
DR   HOGENOM; CLU_022160_0_0_1; -.
DR   InParanoid; Q0J9J6; -.
DR   OMA; HAIQPCT; -.
DR   OrthoDB; 875523at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000007752; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q0J9J6; OS.
DR   GO; GO:0048476; C:Holliday junction resolvase complex; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0010332; P:response to gamma radiation; IEP:UniProtKB.
DR   GO; GO:0009644; P:response to high light intensity; IEP:UniProtKB.
DR   GO; GO:0009411; P:response to UV; IEP:UniProtKB.
DR   Gene3D; 1.10.150.670; -; 1.
DR   InterPro; IPR042530; EME1/EME2_C.
DR   InterPro; IPR006166; ERCC4_domain.
DR   InterPro; IPR033310; Mms4/EME1/EME2.
DR   PANTHER; PTHR21077; PTHR21077; 1.
DR   Pfam; PF02732; ERCC4; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Cell cycle; Cell division; Coiled coil; DNA damage;
KW   DNA recombination; DNA repair; Endonuclease; Hydrolase; Magnesium; Meiosis;
KW   Metal-binding; Mitosis; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..650
FT                   /note="Crossover junction endonuclease EME1"
FT                   /id="PRO_0000418429"
FT   DOMAIN          391..592
FT                   /note="ERCC4"
FT   REGION          1..94
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          107..168
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          220..326
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          291..355
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        235..265
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        287..326
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   650 AA;  71743 MW;  A9D9E740A9110A94 CRC64;
     MASHAAVVEI IDDDDDDDDD VAAASTPPAL HKRSHAVAAA APDSPDAFSP SPPDPKRRQL
     AASTIVLDDT PTPPKRRPPP VAADRSASVV ADTPRSFVPC SLRNRAIAGD TPDSVLPSPS
     FHLDAPDSAT PGSDVPCSVG LDDIVPETPG FNSPRLARPP AVPGLTSPMT ARKFSGVSCP
     ISLDSDDELD DTVYRESLTR TPSNMAKPEH AIQPCTTSCT DKVENTKSTD KKDYSKSNVG
     YQTNNSACKN NGTTSYNQPP RANSPCEDST LKEADPFINN HCPQEENALP IEERKKKQQE
     EKRLKKEKKA REIEEKKQKR LETKKQKEAM KAELAELKKL EKEKKKWESG KLATKCIVAE
     IDSSVIESGS VGGHLVQGFH EKGLCFRVTS NSIKGSILWK MQIPNEFTQD QASTSQVPYI
     LFVLQAEEFC DLVSGGTLLD HVQKVRRQYP EFTICYVTNK LMSFIKRREQ NQYNKTTSNS
     NSWKRPPVEE ALCKLATHYA RVRSRHCTDE AEVTEHIVGL TYSLANCKFR KPLTWLSVHA
     NGSNISKGCV DKDRIKKSAW LKSLVAIPGV SPGQAIAIEK KYPSMRSLLN VYMDDSKSVH
     EKEHLLEDLR LEGPLGDFKR RLGPACSKKV YTILMAQNGA AEVEVDRRGA