ID   EME2_MOUSE              Reviewed;         373 AA.
AC   Q56A04;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Probable crossover junction endonuclease EME2;
DE            EC=3.1.22.-;
GN   Name=Eme2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-373.
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Interacts with MUS81 to form a DNA structure-specific
CC       endonuclease which cleaves substrates such as 3'-flap structures.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with MUS81. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the EME1/MMS4 family. {ECO:0000305}.
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DR   EMBL; AC166102; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC092228; AAH92228.1; -; mRNA.
DR   CCDS; CCDS50023.1; -.
DR   RefSeq; NP_001156574.1; NM_001163102.1.
DR   AlphaFoldDB; Q56A04; -.
DR   SMR; Q56A04; -.
DR   ComplexPortal; CPX-587; Deoxyribonuclease complex MUS81-EME2.
DR   STRING; 10090.ENSMUSP00000113936; -.
DR   PhosphoSitePlus; Q56A04; -.
DR   EPD; Q56A04; -.
DR   PaxDb; Q56A04; -.
DR   PeptideAtlas; Q56A04; -.
DR   PRIDE; Q56A04; -.
DR   ProteomicsDB; 277833; -.
DR   Antibodypedia; 65797; 91 antibodies from 16 providers.
DR   Ensembl; ENSMUST00000121542; ENSMUSP00000113936; ENSMUSG00000073436.
DR   GeneID; 193838; -.
DR   KEGG; mmu:193838; -.
DR   UCSC; uc008ayw.2; mouse.
DR   CTD; 197342; -.
DR   MGI; MGI:1919889; Eme2.
DR   VEuPathDB; HostDB:ENSMUSG00000073436; -.
DR   eggNOG; ENOG502RQYN; Eukaryota.
DR   GeneTree; ENSGT00530000063937; -.
DR   HOGENOM; CLU_034099_1_0_1; -.
DR   InParanoid; Q56A04; -.
DR   OMA; CRIEPQR; -.
DR   OrthoDB; 1595761at2759; -.
DR   PhylomeDB; Q56A04; -.
DR   TreeFam; TF325310; -.
DR   Reactome; R-MMU-5693568; Resolution of D-loop Structures through Holliday Junction Intermediates.
DR   Reactome; R-MMU-6783310; Fanconi Anemia Pathway.
DR   BioGRID-ORCS; 193838; 2 hits in 111 CRISPR screens.
DR   PRO; PR:Q56A04; -.
DR   Proteomes; UP000000589; Chromosome 17.
DR   RNAct; Q56A04; protein.
DR   Bgee; ENSMUSG00000073436; Expressed in humerus cartilage element and 158 other tissues.
DR   ExpressionAtlas; Q56A04; baseline and differential.
DR   Genevisible; Q56A04; MM.
DR   GO; GO:1905347; C:endodeoxyribonuclease complex; ISO:MGI.
DR   GO; GO:0048476; C:Holliday junction resolvase complex; IBA:GO_Central.
DR   GO; GO:0043596; C:nuclear replication fork; IC:ComplexPortal.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006302; P:double-strand break repair; ISO:MGI.
DR   GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0031297; P:replication fork processing; ISO:MGI.
DR   GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR   Gene3D; 1.10.150.670; -; 1.
DR   InterPro; IPR042530; EME1/EME2_C.
DR   InterPro; IPR006166; ERCC4_domain.
DR   InterPro; IPR033310; Mms4/EME1/EME2.
DR   PANTHER; PTHR21077; PTHR21077; 1.
DR   Pfam; PF02732; ERCC4; 1.
DR   SMART; SM00891; ERCC4; 1.
PE   2: Evidence at transcript level;
KW   DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW   Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..373
FT                   /note="Probable crossover junction endonuclease EME2"
FT                   /id="PRO_0000317374"
FT   REGION          24..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   373 AA;  41083 MW;  F0421840E16AE663 CRC64;
     MAEVGPGRVT VSRLGRGLRL GHRRPQTWEI SDSDGEGVPA REVGTQAPSP AGERRAAAKA
     LRADQVLGRL VVCVDPAVLE DAGSDILMEA LGTLGCECRI EPQHQARSLQ WNVVRPDPAP
     SNVPLEAKAE NEQEQLLLLE PQEFLQGAAQ LTQITDPPCS IPWLSPKSLT RSHLAVIGLD
     AYLWSHQLSS QKTWQLKKSK EAHARGAISW AEVEEILVLL QLHANLDVLL MASWQELSQY
     VCAFTRALSQ LPSKQHRDSQ AFSFCTAGHW ASGQQVTRDG SGLRGVWWRQ IRQFNRVSPA
     VADAVVTAFP SPRLLQQALL DCSTEQERLS LLADLPVKAH KGKQPRRVGP DLSRRICIFL
     TTTNPDLLLD LSS