ID   EME2_XENTR              Reviewed;         503 AA.
AC   Q0IHN5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Probable crossover junction endonuclease EME2;
DE            EC=3.1.22.-;
GN   Name=eme2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=N6; TISSUE=Lung;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Interacts with mus81 to form a DNA structure-specific
CC       endonuclease which cleaves substrates such as 3'-flap structures.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with mus81. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the EME1/MMS4 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC123066; AAI23067.1; -; mRNA.
DR   RefSeq; NP_001072860.1; NM_001079392.1.
DR   RefSeq; XP_012826030.1; XM_012970576.2.
DR   AlphaFoldDB; Q0IHN5; -.
DR   SMR; Q0IHN5; -.
DR   STRING; 8364.ENSXETP00000060065; -.
DR   PaxDb; Q0IHN5; -.
DR   DNASU; 780321; -.
DR   Ensembl; ENSXETT00000060853; ENSXETP00000060065; ENSXETG00000031981.
DR   GeneID; 780321; -.
DR   KEGG; xtr:780321; -.
DR   CTD; 197342; -.
DR   Xenbase; XB-GENE-5926460; eme2.
DR   eggNOG; ENOG502RQYN; Eukaryota.
DR   HOGENOM; CLU_034099_1_0_1; -.
DR   InParanoid; Q0IHN5; -.
DR   OMA; CRIEPQR; -.
DR   OrthoDB; 1595761at2759; -.
DR   PhylomeDB; Q0IHN5; -.
DR   TreeFam; TF325310; -.
DR   Proteomes; UP000008143; Chromosome 9.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000031981; Expressed in 2-cell stage embryo and 13 other tissues.
DR   GO; GO:0048476; C:Holliday junction resolvase complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR   GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0031297; P:replication fork processing; IBA:GO_Central.
DR   GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR   Gene3D; 1.10.150.670; -; 1.
DR   InterPro; IPR042530; EME1/EME2_C.
DR   InterPro; IPR006166; ERCC4_domain.
DR   InterPro; IPR033310; Mms4/EME1/EME2.
DR   PANTHER; PTHR21077; PTHR21077; 1.
DR   Pfam; PF02732; ERCC4; 1.
DR   SMART; SM00891; ERCC4; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; DNA damage; DNA recombination; DNA repair; Endonuclease;
KW   Hydrolase; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..503
FT                   /note="Probable crossover junction endonuclease EME2"
FT                   /id="PRO_0000317375"
FT   REGION          1..135
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          103..151
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..18
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        25..44
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        96..135
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   503 AA;  57472 MW;  D8E701F546F55869 CRC64;
     METKQERETG RSSPDPSPRK LIRRAATWEI SDSDNEEETN KPQTESRKGP IVNIEATDQL
     LGVQKDPAQQ PDVEEKTKNL VLPPPTSTTP SPTRRSRKKK TPEQVAAEQE QAEEKKRQRE
     LKRQEKAQKK ELEKIERERR KETNLALKLL RPDQCGKYMV VKVDAGLLED AGSEDVLEAL
     RVAEYNYSIE PHSVPQSISW RREMPADWTC IEGMDMKEEE EDQLLVLVEP KSYLSSVRTY
     AQAPYYFCVG NEMEEIPGSV FSIPAKNPHK KVTLVIIGLQ EYRWCERLSR QIQRQSLDAA
     EGRDSNKDQS ATRATRQQIQ EALVFLQLHY NTEVLCLDTW KELGQHVCAV TKSIAQRPFR
     KHWEAQTFSF CTSAGSWRGW GPRGVLTGLP LTWRRQIQQL NRVSPAMAAV VSQAYPSPQL
     LMQAYSACGS DRERMSLLTD LRIPQDNNTG VTQKAEDPRE VADIAQEGDQ VPGRERRIGP
     DLSRRIWLLM TSKNPELVLD LNS