EMF2A_ORYSJ
ID EMF2A_ORYSJ Reviewed; 624 AA.
AC Q0JF48; Q6VY02; Q7XSN0;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Polycomb group protein EMF2A {ECO:0000305};
DE Short=OsEMF2A {ECO:0000303|PubMed:19825651};
DE AltName: Full=Polycomb group protein EMBRYONIC FLOWER 2B {ECO:0000305};
GN Name=EMF2A {ECO:0000303|PubMed:19825651};
GN OrderedLocusNames=Os04g0162100 {ECO:0000312|EMBL:BAS87849.1},
GN LOC_Os04g08034 {ECO:0000305};
GN ORFNames=OsJ_13761 {ECO:0000312|EMBL:EEE60482.1},
GN OSJNBb0028M18.7 {ECO:0000312|EMBL:CAE01874.2};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Leaf;
RX PubMed=16753820; DOI=10.1080/10425170500151961;
RA Li K., Yang J., Liu J., Du X., Wei C., Su W., He G., Zhang Q., Hong F.,
RA Qian X.;
RT "Cloning, characterization and tissue-specific expression of a cDNA
RT encoding a novel EMBRYONIC FLOWER 2 gene (OsEMF2) in Oryza sativa.";
RL DNA Seq. 17:74-78(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=19825651; DOI=10.1093/mp/ssp036;
RA Luo M., Platten D., Chaudhury A., Peacock W.J., Dennis E.S.;
RT "Expression, imprinting, and evolution of rice homologs of the polycomb
RT group genes.";
RL Mol. Plant 2:711-723(2009).
CC -!- FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming
CC multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. PcG proteins are not required to initiate repression, but
CC to maintain it during later stages of development. They act via the
CC methylation of histones, rendering chromatin heritably changed in its
CC expressibility. {ECO:0000250|UniProtKB:Q69KN0}.
CC -!- SUBUNIT: Component of the polycomb repressive complex 2 (PRC2), which
CC methylates 'Lys-27' residues of histone H3 (H3K27me3), leading to
CC transcriptional repression of the affected target gene.
CC {ECO:0000250|UniProtKB:Q69KN0}.
CC -!- TISSUE SPECIFICITY: Widely expressed (PubMed:19825651). Highly
CC expressed in shoot apical meristem and inflorescence meristem.
CC Expressed in roots, leaves and immature seeds (PubMed:16753820).
CC {ECO:0000269|PubMed:16753820, ECO:0000269|PubMed:19825651}.
CC -!- SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE01874.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EEE60482.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY321106; AAQ84239.1; -; mRNA.
DR EMBL; AL606654; CAE01874.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAF14039.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS87849.1; -; Genomic_DNA.
DR EMBL; CM000141; EEE60482.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_015636445.1; XM_015780959.1.
DR RefSeq; XP_015636446.1; XM_015780960.1.
DR RefSeq; XP_015636447.1; XM_015780961.1.
DR RefSeq; XP_015636448.1; XM_015780962.1.
DR RefSeq; XP_015636449.1; XM_015780963.1.
DR RefSeq; XP_015636450.1; XM_015780964.1.
DR RefSeq; XP_015636451.1; XM_015780965.1.
DR AlphaFoldDB; Q0JF48; -.
DR STRING; 4530.OS04T0162100-01; -.
DR PaxDb; Q0JF48; -.
DR PRIDE; Q0JF48; -.
DR EnsemblPlants; Os04t0162100-01; Os04t0162100-01; Os04g0162100.
DR GeneID; 4335036; -.
DR Gramene; Os04t0162100-01; Os04t0162100-01; Os04g0162100.
DR KEGG; osa:4335036; -.
DR eggNOG; KOG2350; Eukaryota.
DR HOGENOM; CLU_022782_1_0_1; -.
DR InParanoid; Q0JF48; -.
DR OMA; YHLPATH; -.
DR OrthoDB; 516359at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR ExpressionAtlas; Q0JF48; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR InterPro; IPR019135; Polycomb_protein_VEFS-Box.
DR Pfam; PF09733; VEFS-Box; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 2: Evidence at transcript level;
KW Chromatin regulator; Developmental protein; Differentiation; Metal-binding;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..624
FT /note="Polycomb group protein EMF2A"
FT /id="PRO_0000444463"
FT ZN_FING 338..359
FT /note="C2H2-type"
FT /evidence="ECO:0000255"
FT REGION 420..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 474..609
FT /note="VEFS-box"
FT /evidence="ECO:0000255"
FT COMPBIAS 431..445
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 477
FT /note="N -> S (in Ref. 1; AAQ84239)"
FT /evidence="ECO:0000305"
FT CONFLICT 618
FT /note="R -> G (in Ref. 1; AAQ84239)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 624 AA; 71547 MW; 0D8309FE55B0707E CRC64;
MPGLPLTDHD AVNTGCEFDC QRSSDQMCCE HSVAQFSSDQ QLNPEENLAL YCKPLELYNF
IRHRAIENPP YLQRCLLYKI RAKQKKRIQI TISLPGSNNK ELQAQNIFPL YVLFARPTSN
VPIEGHSPIY RFSQARLLTS FNDSGNNDRA EATFVIPDLE TLIATQAYGL TFILVSRGTK
KNKGRTGQNL CENDCSEKHV DYSSLRKLAG KCFWGKIPIT LLNSSLETCA DLILGHIVES
PISICMSPGY LEPTFLEHDN CLSFCSRKAD AMVPYQLQVK VSAAEAGAKD ILKSPYNSFS
YSDVPPSLLL RIVRLRVGNV LFNYKNTQMS EVTEDFTCPF CLVRCGNFKG LECHMTSSHD
LFHYEFWISE DYQAVNVTLK KDNMRTEFVA AEVDNSHRIF YYRSRFKKSR TEILPVARAD
AHIMESGSPE ETQAESEDDV QEENENALID DSKKLHGSNH SQSEFLAFGK SRKLSANRAD
PRNRLLLQKR QFIHSHKAQP MTFEEVLSDN DSEDEVDDDI ADLEDRRMLD DFVDVTKDEK
RIMHMWNSFI RKQSILADSH VPWACEAFSR HHGEELLENS ALLWGWRMFM IKLWNHSLLS
ARTMDTCNRI LDDIKNERSD PKKQ
