ID   EMF2B_ORYSJ             Reviewed;         604 AA.
AC   Q69KN0; Q0J2S6;
DT   20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Polycomb group protein EMF2B {ECO:0000305};
DE            Short=OsEMF2B {ECO:0000303|PubMed:19825651};
DE   AltName: Full=Polycomb group protein EMBRYONIC FLOWER 2B {ECO:0000305};
GN   Name=EMF2B {ECO:0000303|PubMed:19825651};
GN   OrderedLocusNames=Os09g0306800 {ECO:0000312|EMBL:BAT07361.1},
GN   LOC_Os09g13630 {ECO:0000305};
GN   ORFNames=OSJNBa0064I23.28 {ECO:0000312|EMBL:BAD36510.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=19825651; DOI=10.1093/mp/ssp036;
RA   Luo M., Platten D., Chaudhury A., Peacock W.J., Dennis E.S.;
RT   "Expression, imprinting, and evolution of rice homologs of the polycomb
RT   group genes.";
RL   Mol. Plant 2:711-723(2009).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, AND SUBUNIT.
RX   PubMed=23505380; DOI=10.1371/journal.pgen.1003322;
RA   Nallamilli B.R., Zhang J., Mujahid H., Malone B.M., Bridges S.M., Peng Z.;
RT   "Polycomb group gene OsFIE2 regulates rice (Oryza sativa) seed development
RT   and grain filling via a mechanism distinct from Arabidopsis.";
RL   PLoS Genet. 9:E1003322-E1003322(2013).
CC   -!- FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming
CC       multiprotein complexes, which are required to maintain the
CC       transcriptionally repressive state of homeotic genes throughout
CC       development. PcG proteins are not required to initiate repression, but
CC       to maintain it during later stages of development. They act via the
CC       methylation of histones, rendering chromatin heritably changed in its
CC       expressibility (Probable). Polycomb group (PcG) protein involved in the
CC       repression of flowering under long day (LD) conditions. Regulates
CC       floret development (PubMed:19825651). {ECO:0000269|PubMed:19825651,
CC       ECO:0000305|PubMed:23505380}.
CC   -!- SUBUNIT: Component of the polycomb repressive complex 2 (PRC2),
CC       composed of the core PRC2 components FIE2, EZ1 and CLF. PRC2 methylates
CC       'Lys-27' residues of histone H3 (H3K27me3), leading to transcriptional
CC       repression of the affected target gene. {ECO:0000269|PubMed:23505380}.
CC   -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:19825651}.
CC   -!- DISRUPTION PHENOTYPE: Dwarf phenotype with early flowering under long
CC       day (LD) conditions. Defect in floret development.
CC       {ECO:0000269|PubMed:19825651}.
CC   -!- SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF24739.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAT07361.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AP005912; BAD36510.1; -; Genomic_DNA.
DR   EMBL; AP008215; BAF24739.2; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014965; BAT07361.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_015612401.1; XM_015756915.1.
DR   AlphaFoldDB; Q69KN0; -.
DR   STRING; 4530.OS09T0306800-01; -.
DR   iPTMnet; Q69KN0; -.
DR   PRIDE; Q69KN0; -.
DR   EnsemblPlants; Os09t0306800-01; Os09t0306800-01; Os09g0306800.
DR   GeneID; 4346674; -.
DR   Gramene; Os09t0306800-01; Os09t0306800-01; Os09g0306800.
DR   KEGG; osa:4346674; -.
DR   eggNOG; KOG2350; Eukaryota.
DR   HOGENOM; CLU_022782_1_0_1; -.
DR   InParanoid; Q69KN0; -.
DR   OrthoDB; 516359at2759; -.
DR   Proteomes; UP000000763; Chromosome 9.
DR   Proteomes; UP000059680; Chromosome 9.
DR   GO; GO:0005677; C:chromatin silencing complex; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0031519; C:PcG protein complex; IDA:UniProtKB.
DR   GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0009908; P:flower development; IMP:UniProtKB.
DR   GO; GO:0031507; P:heterochromatin assembly; IDA:UniProtKB.
DR   GO; GO:0070734; P:histone H3-K27 methylation; IDA:UniProtKB.
DR   GO; GO:0048586; P:regulation of long-day photoperiodism, flowering; IMP:UniProtKB.
DR   InterPro; IPR019135; Polycomb_protein_VEFS-Box.
DR   Pfam; PF09733; VEFS-Box; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE   1: Evidence at protein level;
KW   Chromatin regulator; Developmental protein; Differentiation; Flowering;
KW   Metal-binding; Reference proteome; Transcription; Transcription regulation;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..604
FT                   /note="Polycomb group protein EMF2B"
FT                   /id="PRO_0000444464"
FT   ZN_FING         310..331
FT                   /note="C2H2-type"
FT                   /evidence="ECO:0000255"
FT   REGION          396..440
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          454..589
FT                   /note="VEFS-box"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        415..440
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   604 AA;  68616 MW;  8A0D7E642F1D3562 CRC64;
     MCRHQPRARL SPDEQLAAEE SFALYCKPVE LYNIIQRRSI KNPAFLQRCL LYKIHARRKK
     RSLITISLSG GTNKELRAQN IFPLYVLLAR PTNNVSLEGH SPIYRFSRAC LLTSFHEFGN
     KDYTEATFVI PDVKNLATSR ACSLNIILIS CGRAEQTFDD NNCSGNHVEG STLQKLEGKC
     FWGKIPIDLL ASSLGNCVSL SLGHTVEMSS TVEMTPSFLE PKFLEDDSCL TFCSQKVDAT
     GSFQLQVSIS AQEAGAKDMS ESPYSVYSYN DVPPSSLTHI IRLRSGNVLF NYKYYNNTMQ
     KTEVTEDFSC PFCLVPCGSF KGLGCHLNAS HDLFHYEFWI SEECQAVNVS LKTDSWRTEL
     LAEGVDPRHQ TFSYRSRFKK RKRVEISSDK IRHVHPHIVD SGSPEDAQAG SEDDYVQREN
     GSSVAHASVD PANSLHGSNL SAPTVLQFGK TRKLSVERAD PRNRQLLQKR QFFHSHRAQP
     MALEQVFSDR DSEDEVDDDI ADFEDRRMLD DFVDVTKDEK LIMHMWNSFV RKQRVLADGH
     IPWACEAFSQ FHGQELVQNP ALLWCWRFFM VKLWNHSLLD ARAMNACNTI LEGYLNGSSD
     PKKN