EML4_ARATH
ID EML4_ARATH Reviewed; 429 AA.
AC Q08A72; O64878; Q67YT9; Q680Q1;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Protein EMSY-LIKE 4 {ECO:0000303|PubMed:21830950};
DE Short=AtEML4 {ECO:0000303|PubMed:21830950};
GN Name=EML4 {ECO:0000303|PubMed:21830950};
GN OrderedLocusNames=At2g44440 {ECO:0000312|Araport:AT2G44440};
GN ORFNames=F4I1.25 {ECO:0000312|EMBL:AAC16090.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABI93916.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21830950; DOI=10.1094/mpmi-05-11-0123;
RA Tsuchiya T., Eulgem T.;
RT "EMSY-like genes are required for full RPP7-mediated race-specific immunity
RT and basal defense in Arabidopsis.";
RL Mol. Plant Microbe Interact. 24:1573-1581(2011).
CC -!- FUNCTION: Probably involved in the regulation of chromatin states
CC (Probable). Contributes to RPP7-mediated and basal immunity, especially
CC against Hyaloperonospora arabidopsidis isolate Hiks1 (PubMed:21830950).
CC {ECO:0000269|PubMed:21830950, ECO:0000305|PubMed:21830950}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9C7C4,
CC ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- DISRUPTION PHENOTYPE: Reduced resistance to Hyaloperonospora
CC arabidopsidis isolate Hiks1. {ECO:0000269|PubMed:21830950}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC16090.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004521; AAC16090.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10420.1; -; Genomic_DNA.
DR EMBL; AK175816; BAD43579.1; -; mRNA.
DR EMBL; AK176379; BAD44142.1; -; mRNA.
DR EMBL; BT029007; ABI93916.1; -; mRNA.
DR PIR; T02399; T02399.
DR RefSeq; NP_181972.2; NM_130007.4.
DR AlphaFoldDB; Q08A72; -.
DR SMR; Q08A72; -.
DR STRING; 3702.AT2G44440.1; -.
DR iPTMnet; Q08A72; -.
DR PaxDb; Q08A72; -.
DR PRIDE; Q08A72; -.
DR ProteomicsDB; 222661; -.
DR EnsemblPlants; AT2G44440.1; AT2G44440.1; AT2G44440.
DR GeneID; 819051; -.
DR Gramene; AT2G44440.1; AT2G44440.1; AT2G44440.
DR KEGG; ath:AT2G44440; -.
DR Araport; AT2G44440; -.
DR TAIR; locus:2050594; AT2G44440.
DR eggNOG; KOG4675; Eukaryota.
DR HOGENOM; CLU_038636_0_1_1; -.
DR InParanoid; Q08A72; -.
DR OMA; QADLTHQ; -.
DR OrthoDB; 620151at2759; -.
DR PhylomeDB; Q08A72; -.
DR PRO; PR:Q08A72; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q08A72; baseline and differential.
DR Genevisible; Q08A72; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR Gene3D; 1.10.1240.40; -; 1.
DR InterPro; IPR033485; EMSY-LIKE_plant.
DR InterPro; IPR005491; ENT_dom.
DR InterPro; IPR036142; ENT_dom-like_sf.
DR PANTHER; PTHR33432; PTHR33432; 1.
DR Pfam; PF03735; ENT; 1.
DR SMART; SM01191; ENT; 1.
DR SUPFAM; SSF158639; SSF158639; 1.
DR PROSITE; PS51138; ENT; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Nucleus; Phosphoprotein; Plant defense; Reference proteome;
KW Repeat.
FT CHAIN 1..429
FT /note="Protein EMSY-LIKE 4"
FT /id="PRO_0000431794"
FT DOMAIN 57..144
FT /note="ENT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00476"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 146..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 48..113
FT /evidence="ECO:0000255"
FT COILED 393..415
FT /evidence="ECO:0000255"
FT MOTIF 190..197
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 349..356
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..247
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 419
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:F4K2F0"
FT CONFLICT 369
FT /note="R -> Q (in Ref. 3; BAD43579)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 429 AA; 47454 MW; FDC89662E4E47BA3 CRC64;
MDCKSSDSSG TDDDLPPSHR VPRGGGGGRG GRVAGNGRPL NLPPSYPKMY DDLAADMEAQ
IHQIEKEAYI SVLRAFKAQG DAISWEKESV ITELRKELSL SNEEHRELLG RVNSDDTIRR
IREWRQSGGM QPSMRNAAQV VHDTLPSPSV SASMKTHKPN QPIPSQPFAS SSPSFHPQAD
PTHPFASSTA KRGPVPIVKG KKHKPVFPGS SSTKHAPYHP SDQPPRGQVM NRLPSVPASS
SEPTNGIDPE SFLGRRVRTK WPEDNTFYDA IITQYNPVEG RHALVYDIAT PSETWEWVRL
SEISPGDIEW IGEDPGLGNR YNGQGHGLNR TTGPNCVPQR GSGLEKNTIR KGFRTSQNGT
GKKKHLDIRI RQTDVLIREV ERVLRSHNPD PYEVERAKRV LEEHEHALVG AIAKLGDISD
GENEGAFRR
