EMP2_PANTR
ID EMP2_PANTR Reviewed; 167 AA.
AC A5A6N6;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Epithelial membrane protein 2;
DE Short=EMP-2;
GN Name=EMP2;
OS Pan troglodytes (Chimpanzee).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pan.
OX NCBI_TaxID=9598;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skin;
RX PubMed=17574350; DOI=10.1016/j.gene.2007.04.013;
RA Sakate R., Suto Y., Imanishi T., Tanoue T., Hida M., Hayasaka I.,
RA Kusuda J., Gojobori T., Hashimoto K., Hirai M.;
RT "Mapping of chimpanzee full-length cDNAs onto the human genome unveils
RT large potential divergence of the transcriptome.";
RL Gene 399:1-10(2007).
CC -!- FUNCTION: Functions as a key regulator of cell membrane composition by
CC regulating protein surface expression. Also, plays a role in regulation
CC of processes including cell migration, cell proliferation, cell
CC contraction and cell adhesion. Regulates transepithelial migration of
CC neutrophils into the alveolar lumen, potentially via mediation of cell
CC surface expression of adhesion markers and lipid raft formation (By
CC similarity). Negatively regulates caveolae formation by reducing CAV1
CC expression and CAV1 amount by increasing lysosomal degradation (By
CC similarity). Facilitates surface trafficking and the formation of lipid
CC rafts bearing GPI-anchor proteins (By similarity). Regulates surface
CC expression of MHC1 and ICAM1 proteins increasing susceptibility to T-
CC cell mediated cytotoxicity (By similarity). Regulates the plasma
CC membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-
CC ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion
CC (By similarity). Also regulates many processes through PTK2 (By
CC similarity). Regulates blood vessel endothelial cell migration and
CC angiogenesis by regulating VEGF protein expression through PTK2
CC activation (By similarity). Regulates cell migration and cell
CC contraction through PTK2 and SRC activation. Regulates focal adhesion
CC density, F-actin conformation and cell adhesion capacity through
CC interaction with PTK2 (By similarity). Positively regulates cell
CC proliferation (By similarity). Plays a role during cell death and cell
CC blebbing (By similarity). Promotes angiogenesis and vasculogenesis
CC through induction of VEGFA via a HIF1A-dependent pathway (By
CC similarity). Also plays a role in embryo implantation by regulating
CC surface trafficking of integrin heterodimer ITGA5-ITGB3 (By
CC similarity). Plays a role in placental angiogenesis and uterine natural
CC killer cell regulation at the maternal-fetal placental interface,
CC however not required in the maternal tissues for a viable pregnancy (By
CC similarity). Involved in the early stages of embryogenic development
CC and cardiogenesis, potentially via regulation of epithelial-mesenchymal
CC transition timing (By similarity). May play a role in glomerular
CC filtration (By similarity). {ECO:0000250|UniProtKB:F1QIK8,
CC ECO:0000250|UniProtKB:O88662, ECO:0000250|UniProtKB:P54851}.
CC -!- SUBUNIT: Interacts with PTK2; regulates PTK2 activation and
CC localization (By similarity). Interacts with ITGB3; regulates the
CC levels of the heterodimer ITGA5-ITGB3 integrin surface expression (By
CC similarity). Interacts with P2RX7 (via C-terminus) (By similarity).
CC Interacts with ITGB1; the interaction may be direct or indirect and
CC ITGB1 has a heterodimer form (By similarity).
CC {ECO:0000250|UniProtKB:O88662, ECO:0000250|UniProtKB:P54851}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:O88662}; Multi-pass membrane protein
CC {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:O88662,
CC ECO:0000250|UniProtKB:P54851}. Apical cell membrane
CC {ECO:0000250|UniProtKB:O88662}. Membrane raft
CC {ECO:0000250|UniProtKB:O88662, ECO:0000250|UniProtKB:P54851}. Cytoplasm
CC {ECO:0000250|UniProtKB:O88662, ECO:0000250|UniProtKB:P54851,
CC ECO:0000250|UniProtKB:Q66HH2}. Nucleus {ECO:0000250|UniProtKB:Q66HH2}.
CC Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O88662}.
CC Note=Localizes in cytoplasm, foot processes and cell bodies of
CC podocytes and nucleus of endothelial cells of kidney. Localizes to the
CC apical cell surface in the luminal epithelium and glandular epithelium.
CC Colocalized with ITGB1 and GPI-anchor proteins on plasma membrane on
CC plasma membrane. {ECO:0000250|UniProtKB:O88662,
CC ECO:0000250|UniProtKB:Q66HH2}.
CC -!- SIMILARITY: Belongs to the PMP-22/EMP/MP20 family. {ECO:0000305}.
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DR EMBL; AB222164; BAF62409.1; -; mRNA.
DR RefSeq; NP_001098709.1; NM_001105239.1.
DR RefSeq; XP_009428554.1; XM_009430279.2.
DR RefSeq; XP_016783942.1; XM_016928453.1.
DR AlphaFoldDB; A5A6N6; -.
DR SMR; A5A6N6; -.
DR STRING; 9598.ENSPTRP00000013245; -.
DR PaxDb; A5A6N6; -.
DR Ensembl; ENSPTRT00000091043; ENSPTRP00000090573; ENSPTRG00000007756.
DR GeneID; 453910; -.
DR KEGG; ptr:453910; -.
DR CTD; 2013; -.
DR VGNC; VGNC:13702; EMP2.
DR eggNOG; ENOG502RYYE; Eukaryota.
DR GeneTree; ENSGT00950000182696; -.
DR HOGENOM; CLU_138632_0_0_1; -.
DR InParanoid; A5A6N6; -.
DR OMA; ADIWRVC; -.
DR OrthoDB; 1345659at2759; -.
DR TreeFam; TF330414; -.
DR Proteomes; UP000002277; Chromosome 16.
DR Bgee; ENSPTRG00000007756; Expressed in lung and 21 other tissues.
DR GO; GO:0045177; C:apical part of cell; ISS:UniProtKB.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0009986; C:cell surface; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045121; C:membrane raft; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005178; F:integrin binding; IEA:Ensembl.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; ISS:UniProtKB.
DR GO; GO:0070252; P:actin-mediated cell contraction; ISS:UniProtKB.
DR GO; GO:0032147; P:activation of protein kinase activity; ISS:UniProtKB.
DR GO; GO:0032060; P:bleb assembly; ISS:UniProtKB.
DR GO; GO:0043534; P:blood vessel endothelial cell migration; ISS:UniProtKB.
DR GO; GO:0007155; P:cell adhesion; ISS:UniProtKB.
DR GO; GO:0008219; P:cell death; ISS:UniProtKB.
DR GO; GO:0016477; P:cell migration; ISS:UniProtKB.
DR GO; GO:0007160; P:cell-matrix adhesion; ISS:UniProtKB.
DR GO; GO:0045022; P:early endosome to late endosome transport; ISS:UniProtKB.
DR GO; GO:0007566; P:embryo implantation; ISS:UniProtKB.
DR GO; GO:0060136; P:embryonic process involved in female pregnancy; ISS:UniProtKB.
DR GO; GO:0060914; P:heart formation; ISS:UniProtKB.
DR GO; GO:0001765; P:membrane raft assembly; ISS:UniProtKB.
DR GO; GO:0001787; P:natural killer cell proliferation; ISS:UniProtKB.
DR GO; GO:1990266; P:neutrophil migration; ISS:UniProtKB.
DR GO; GO:0044854; P:plasma membrane raft assembly; ISS:UniProtKB.
DR GO; GO:0045766; P:positive regulation of angiogenesis; ISS:UniProtKB.
DR GO; GO:0062043; P:positive regulation of cardiac epithelial to mesenchymal transition; ISS:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0001954; P:positive regulation of cell-matrix adhesion; IEA:Ensembl.
DR GO; GO:2001046; P:positive regulation of integrin-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0034394; P:protein localization to cell surface; ISS:UniProtKB.
DR GO; GO:0072659; P:protein localization to plasma membrane; ISS:UniProtKB.
DR GO; GO:0045765; P:regulation of angiogenesis; ISS:UniProtKB.
DR GO; GO:0001952; P:regulation of cell-matrix adhesion; IBA:GO_Central.
DR GO; GO:0010594; P:regulation of endothelial cell migration; ISS:UniProtKB.
DR GO; GO:0003093; P:regulation of glomerular filtration; ISS:UniProtKB.
DR GO; GO:0043549; P:regulation of kinase activity; ISS:UniProtKB.
DR GO; GO:2001212; P:regulation of vasculogenesis; ISS:UniProtKB.
DR GO; GO:0001913; P:T cell mediated cytotoxicity; ISS:UniProtKB.
DR InterPro; IPR003933; EMP-2.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR InterPro; IPR004032; PMP22_EMP_MP20.
DR PANTHER; PTHR10671:SF32; PTHR10671:SF32; 1.
DR Pfam; PF00822; PMP22_Claudin; 1.
DR PRINTS; PR01453; EPMEMFAMILY.
DR PRINTS; PR01455; EPMEMPROT2.
DR PROSITE; PS01221; PMP22_1; 1.
DR PROSITE; PS01222; PMP22_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasm; Glycoprotein; Golgi apparatus; Membrane; Nucleus;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..167
FT /note="Epithelial membrane protein 2"
FT /id="PRO_0000297551"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 44
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 167 AA; 19235 MW; 831D7EEC64F4D061 CRC64;
MLVLLAFIIA FHITSAALLF IATIDNAWWV GDEFFADVWR ICTNNTNCTV INDSFQEYST
LQAVQATMIL STILCCIAFF IFVLQLFRLK QGERFVLTSI IQLMSCLCVM IAASIYTDRR
EDIHHKNAKF YPVTREGSYG YSYILAWVAF ACTFISGMMY LILRKRK
