EMRE_BOVIN
ID EMRE_BOVIN Reviewed; 107 AA.
AC Q2M2S2;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2006, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Essential MCU regulator, mitochondrial {ECO:0000250|UniProtKB:Q9H4I9};
DE AltName: Full=Single-pass membrane protein with aspartate-rich tail 1, mitochondrial {ECO:0000250|UniProtKB:Q9H4I9};
DE Flags: Precursor;
GN Name=SMDT1 {ECO:0000250|UniProtKB:Q9H4I9};
GN Synonyms=EMRE {ECO:0000250|UniProtKB:Q9H4I9};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Heart ventricle;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential regulatory subunit of the mitochondrial calcium
CC uniporter complex (uniplex), a complex that mediates calcium uptake
CC into mitochondria. Required to bridge the calcium-sensing proteins
CC MICU1 and MICU2 with the calcium-conducting subunit MCU. Plays a
CC central role in regulating the uniplex complex response to
CC intracellular calcium signaling. Acts by mediating activation of MCU
CC and retention of MICU1 to the MCU pore, in order to ensure tight
CC regulation of the uniplex complex and appropriate responses to
CC intracellular calcium signaling. {ECO:0000250|UniProtKB:Q9H4I9}.
CC -!- SUBUNIT: Component of the uniplex complex, composed of MCU, MCUB,
CC MICU1, MICU2 and EMRE/SMDT1. Interacts (via the transmembrane region)
CC with MCU (via the first transmembrane region); the interaction is
CC direct. Interacts (via the poly-Asp region) with MICU1 (via polybasic
CC region); the interaction is direct. Interacts (via its C-terminal poly-
CC Asp tail) with MCUR1; the interaction is direct. Unprocessed form
CC interacts (via transit peptide) with MAIP1.
CC {ECO:0000250|UniProtKB:Q9H4I9}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q9H4I9}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9H4I9}. Note=MAIP1 is required to assist
CC sorting of EMRE/SMDT1 into mitochondrion by protecting EMRE/SMDT1
CC against protein degradation by YME1L1, thereby ensuring SMDT1/EMRE
CC maturation by the mitochondrial processing peptidase (PMPCA and PMPCB).
CC {ECO:0000250|UniProtKB:Q9H4I9}.
CC -!- DOMAIN: The GXXXX[G/A/S] motif at the C-terminal part of the
CC transmembrane region mediates interaction with MCU and is required to
CC activate the calcium-conducting pore in the uniporter complex.
CC {ECO:0000250|UniProtKB:Q9H4I9}.
CC -!- DOMAIN: The poly-Asp region at the C-terminus mediates interaction with
CC the polybasic region of MICU1. {ECO:0000250|UniProtKB:Q9H4I9}.
CC -!- PTM: Undergoes proteolytic degradation in neurons: degraded by AFG3L2
CC before SMDT1/EMRE assembly with the uniporter complex, limiting the
CC availability of SMDT1/EMRE for MCU assembly and promoting efficient
CC assembly of gatekeeper subunits with MCU.
CC {ECO:0000250|UniProtKB:Q9H4I9}.
CC -!- SIMILARITY: Belongs to the SMDT1/EMRE family. {ECO:0000305}.
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DR EMBL; BC111682; AAI11683.1; -; mRNA.
DR RefSeq; NP_001039402.1; NM_001045937.1.
DR AlphaFoldDB; Q2M2S2; -.
DR SMR; Q2M2S2; -.
DR STRING; 9913.ENSBTAP00000012511; -.
DR PaxDb; Q2M2S2; -.
DR Ensembl; ENSBTAT00000012511; ENSBTAP00000012511; ENSBTAG00000009508.
DR GeneID; 506149; -.
DR KEGG; bta:506149; -.
DR CTD; 91689; -.
DR VEuPathDB; HostDB:ENSBTAG00000009508; -.
DR VGNC; VGNC:35009; SMDT1.
DR eggNOG; KOG4542; Eukaryota.
DR GeneTree; ENSGT00390000017489; -.
DR HOGENOM; CLU_172921_1_0_1; -.
DR InParanoid; Q2M2S2; -.
DR OMA; CISKNFA; -.
DR OrthoDB; 1586309at2759; -.
DR TreeFam; TF314649; -.
DR Proteomes; UP000009136; Chromosome 5.
DR Bgee; ENSBTAG00000009508; Expressed in ileocecal valve and 103 other tissues.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:1990246; C:uniplex complex; ISS:UniProtKB.
DR GO; GO:0036444; P:calcium import into the mitochondrion; ISS:UniProtKB.
DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; ISS:UniProtKB.
DR GO; GO:0006851; P:mitochondrial calcium ion transmembrane transport; ISS:UniProtKB.
DR InterPro; IPR018782; MCU_reg.
DR PANTHER; PTHR33904; PTHR33904; 1.
DR Pfam; PF10161; DDDD; 1.
PE 3: Inferred from homology;
KW Calcium; Calcium transport; Ion transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..47
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 48..107
FT /note="Essential MCU regulator, mitochondrial"
FT /id="PRO_0000296322"
FT TOPO_DOM 48..64
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:Q9H4I9"
FT TRANSMEM 65..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..107
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:Q9H4I9"
FT MOTIF 81..85
FT /note="GXXXX[G/A/S]"
FT /evidence="ECO:0000250|UniProtKB:Q9H4I9"
SQ SEQUENCE 107 AA; 11450 MW; D0678EAC1B8178F5 CRC64;
MASGAARWLA LVRVGSGASR SWLSLRKGGD VSAGRSCSGQ SLVPTRSVIV TRSGAILPKP
VKMSFGLLRV FSIVIPFLYV GTLISKNFAA LLEEHDIFVP EDDDDDD
