EMRE_DROME
ID EMRE_DROME Reviewed; 97 AA.
AC Q7JX57;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Essential MCU regulator, mitochondrial {ECO:0000250|UniProtKB:Q9H4I9};
DE Flags: Precursor;
GN Name=EMRE {ECO:0000303|PubMed:27099988, ECO:0000312|FlyBase:FBgn0062440};
GN ORFNames=CG17680 {ECO:0000312|FlyBase:FBgn0062440};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION.
RX PubMed=27099988; DOI=10.7554/elife.15545;
RA Tsai M.F., Phillips C.B., Ranaghan M., Tsai C.W., Wu Y., Willliams C.,
RA Miller C.;
RT "Dual functions of a small regulatory subunit in the mitochondrial calcium
RT uniporter complex.";
RL Elife 5:0-0(2016).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28726639; DOI=10.1074/jbc.m116.765578;
RA Choi S., Quan X., Bang S., Yoo H., Kim J., Park J., Park K.S., Chung J.;
RT "Mitochondrial calcium uniporter in Drosophila transfers calcium between
RT the endoplasmic reticulum and mitochondria in oxidative stress-induced cell
RT death.";
RL J. Biol. Chem. 292:14473-14485(2017).
CC -!- FUNCTION: Essential regulatory subunit of the mitochondrial calcium
CC uniporter MCU channel, a protein that mediates calcium uptake into
CC mitochondria. {ECO:0000269|PubMed:27099988,
CC ECO:0000269|PubMed:28726639}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q9H4I9}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9H4I9}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in muscle results in
CC mitochondrial calcium uptake defects. {ECO:0000269|PubMed:28726639}.
CC -!- SIMILARITY: Belongs to the SMDT1/EMRE family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE013599; AAF57742.1; -; Genomic_DNA.
DR EMBL; AY095085; AAM11413.1; -; mRNA.
DR RefSeq; NP_611294.1; NM_137450.2.
DR AlphaFoldDB; Q7JX57; -.
DR SMR; Q7JX57; -.
DR STRING; 7227.FBpp0085960; -.
DR TCDB; 8.A.45.1.2; the essential mcu regulator emre (emre) family.
DR PaxDb; Q7JX57; -.
DR PRIDE; Q7JX57; -.
DR DNASU; 37071; -.
DR EnsemblMetazoa; FBtr0086781; FBpp0085960; FBgn0062440.
DR GeneID; 37071; -.
DR KEGG; dme:Dmel_CG17680; -.
DR UCSC; CG17680-RA; d. melanogaster.
DR CTD; 37071; -.
DR FlyBase; FBgn0062440; EMRE.
DR VEuPathDB; VectorBase:FBgn0062440; -.
DR eggNOG; KOG4542; Eukaryota.
DR GeneTree; ENSGT00390000017489; -.
DR HOGENOM; CLU_172921_0_0_1; -.
DR InParanoid; Q7JX57; -.
DR OMA; AFPISHA; -.
DR OrthoDB; 1586309at2759; -.
DR PhylomeDB; Q7JX57; -.
DR Reactome; R-DME-8949215; Mitochondrial calcium ion transport.
DR Reactome; R-DME-8949664; Processing of SMDT1.
DR BioGRID-ORCS; 37071; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37071; -.
DR PRO; PR:Q7JX57; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0062440; Expressed in brain and 11 other tissues.
DR ExpressionAtlas; Q7JX57; baseline and differential.
DR Genevisible; Q7JX57; DM.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:1990246; C:uniplex complex; ISS:FlyBase.
DR GO; GO:0036444; P:calcium import into the mitochondrion; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; HMP:FlyBase.
DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IMP:UniProtKB.
DR GO; GO:0006851; P:mitochondrial calcium ion transmembrane transport; IMP:UniProtKB.
DR GO; GO:0045089; P:positive regulation of innate immune response; HMP:FlyBase.
DR InterPro; IPR018782; MCU_reg.
DR PANTHER; PTHR33904; PTHR33904; 1.
DR Pfam; PF10161; DDDD; 1.
PE 3: Inferred from homology;
KW Calcium; Calcium transport; Ion transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..35
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 36..97
FT /note="Essential MCU regulator, mitochondrial"
FT /id="PRO_0000296325"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 97 AA; 10842 MW; 1120C8AC60C41393 CRC64;
MIVPRLALPI SLALQRVSRR VAEHPHNLRI LQRHMSSVYF RSGAIKPKPE EMPFGLLAIF
CAVIPGLFVG ATISKNVANF LEENDLFVPA DDDDDED
