EMS_DROME
ID EMS_DROME Reviewed; 497 AA.
AC P18488; Q9VFQ1;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Homeotic protein empty spiracles;
GN Name=ems; Synonyms=E4; ORFNames=CG2988;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Oregon-R;
RX PubMed=2576012; DOI=10.1101/gad.3.12a.1940;
RA Dalton D., Chadwick R., McGinnis W.;
RT "Expression and embryonic function of empty spiracles: a Drosophila homeo
RT box gene with two patterning functions on the anterior-posterior axis of
RT the embryo.";
RL Genes Dev. 3:1940-1956(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S;
RX PubMed=1376248; DOI=10.1002/j.1460-2075.1992.tb05284.x;
RA Walldorf U., Gehring W.J.;
RT "Empty spiracles, a gap gene containing a homeobox involved in Drosophila
RT head development.";
RL EMBO J. 11:2247-2259(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Acts as a homeotic selector gene controlling antennal and
CC mandibular segment identity.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: EMS has two different spatial patterns of
CC expression during embryogenesis. The EMS head-specific expression
CC pattern initiates prior to cellular blastoderm and continues only until
CC early germ-band extension. The EMS metameric expression pattern
CC initiates after gastrulation and is expressed in the lateral
CC neuroblasts, in ectodermal cells at the anterior lateral borders of
CC each segment, and in the Filzkoerper primordia.
CC -!- MISCELLANEOUS: The sequence shown is that of strain canton S.
CC -!- SIMILARITY: Belongs to the EMX homeobox family. {ECO:0000305}.
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DR EMBL; X51653; CAA35965.1; -; mRNA.
DR EMBL; X66270; CAA46985.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54999.1; -; Genomic_DNA.
DR PIR; S22708; S22708.
DR RefSeq; NP_731868.1; NM_169560.2.
DR AlphaFoldDB; P18488; -.
DR SMR; P18488; -.
DR BioGRID; 66774; 12.
DR IntAct; P18488; 4.
DR STRING; 7227.FBpp0082326; -.
DR PaxDb; P18488; -.
DR DNASU; 41697; -.
DR GeneID; 41697; -.
DR KEGG; dme:Dmel_CG2988; -.
DR CTD; 41697; -.
DR FlyBase; FBgn0000576; ems.
DR VEuPathDB; VectorBase:FBgn0000576; -.
DR eggNOG; KOG0843; Eukaryota.
DR HOGENOM; CLU_040711_0_0_1; -.
DR InParanoid; P18488; -.
DR SignaLink; P18488; -.
DR BioGRID-ORCS; 41697; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 41697; -.
DR PRO; PR:P18488; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR ExpressionAtlas; P18488; baseline and differential.
DR Genevisible; P18488; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0035288; P:anterior head segmentation; TAS:FlyBase.
DR GO; GO:0007409; P:axonogenesis; IMP:FlyBase.
DR GO; GO:0007420; P:brain development; IMP:FlyBase.
DR GO; GO:0035284; P:brain segmentation; IMP:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0001700; P:embryonic development via the syncytial blastoderm; IMP:FlyBase.
DR GO; GO:0014019; P:neuroblast development; IMP:FlyBase.
DR GO; GO:0007424; P:open tracheal system development; IMP:FlyBase.
DR GO; GO:0007389; P:pattern specification process; NAS:FlyBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0035277; P:spiracle morphogenesis, open tracheal system; IMP:FlyBase.
DR GO; GO:0007419; P:ventral cord development; IMP:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..497
FT /note="Homeotic protein empty spiracles"
FT /id="PRO_0000049059"
FT DNA_BIND 391..450
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 34..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 161..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 441..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..93
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..175
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..234
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..249
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 445..460
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 472..489
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 4
FT /note="M -> T (in strain: Oregon-R)"
FT VARIANT 102..104
FT /note="Missing (in strain: Berkeley and Oregon-R)"
FT VARIANT 131
FT /note="H -> Q (in strain: Berkeley and Oregon-R)"
FT VARIANT 158..159
FT /note="SG -> TR (in strain: Oregon-R)"
FT VARIANT 304..305
FT /note="EL -> DV (in strain: Oregon-R)"
SQ SEQUENCE 497 AA; 53784 MW; BB7E4548155C0A5C CRC64;
MTKMIPPVPT AAAAVMMPTP KQKIGFSIES IVGNDVSTAG GNSTPDLSGP QSPPPGERNV
PGSPPQTPPA TLTLIPGSPP HHLMAPPAHG LPYPHPHAQQ QQQQHLQAPH PHPHLSPAQQ
HVLHQHLLMQ HQHPGTPKSH QDIQELLQRL HHNAAMASGL SPLQTRLSPE TEQPQMAVSL
KRERSPAPPA MEQAENPAQR IQPPHTPPKS VSPQSSQPSS SPTLLISSPH ATPPQQQQQQ
PPPNYPKPAM MHPGGAGPMM MPGMPPAGLV RPFPMGPGGP PMPQGQPGLP DIKALPPYIN
APPELPPQHN PHLIAAAQFQ MAAALQAGHV LGPAAAAAAA AGLPPHAAQF MPNPGMARDS
YQLYPWLLSR HGRIFPHRFP GSFLVPPFRK PKRIRTAFSP SQLLKLEHAF ESNQYVVGAE
RKALAQNLNL SETQVKVWFQ NRRTKHKRMQ QEDEKGGEGG SQRNMHNGSG DEDDDELIDM
EMDECPSDEE HELDASH
