AGAP4_HUMAN
ID AGAP4_HUMAN Reviewed; 663 AA.
AC Q96P64;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 4 {ECO:0000312|HGNC:HGNC:23459};
DE Short=AGAP-4;
DE AltName: Full=Centaurin-gamma-like family member 1 {ECO:0000312|HGNC:HGNC:23459};
DE AltName: Full=Centaurin-gamma-like family member 5 {ECO:0000312|HGNC:HGNC:23459};
GN Name=AGAP4 {ECO:0000312|HGNC:HGNC:23459};
GN Synonyms=AGAP8 {ECO:0000312|HGNC:HGNC:23459},
GN CTGLF1 {ECO:0000312|HGNC:HGNC:23459}, CTGLF5 {ECO:0000312|HGNC:HGNC:23459},
GN MRIP2 {ECO:0000312|EMBL:AAL10290.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT GLU-661.
RA Gong L., Wu K.;
RT "Molecular cloning of MRIP2, a novel gene located on human chromosome 10.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
CC -!- FUNCTION: Putative GTPase-activating protein. {ECO:0000305}.
CC -!- MISCELLANEOUS: Encoded by one of the numerous copies of centaurin
CC gamma-like genes clustered in the q11 region of chromosome 10.
CC -!- SIMILARITY: Belongs to the centaurin gamma-like family. {ECO:0000305}.
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DR EMBL; AF411132; AAL10290.1; -; mRNA.
DR EMBL; AC012044; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS7215.1; -.
DR RefSeq; NP_001263272.2; NM_001276343.2.
DR RefSeq; NP_597703.2; NM_133446.3.
DR AlphaFoldDB; Q96P64; -.
DR SMR; Q96P64; -.
DR BioGRID; 125632; 4.
DR IntAct; Q96P64; 2.
DR MINT; Q96P64; -.
DR STRING; 9606.ENSP00000392513; -.
DR iPTMnet; Q96P64; -.
DR PhosphoSitePlus; Q96P64; -.
DR BioMuta; AGAP4; -.
DR DMDM; 296434392; -.
DR jPOST; Q96P64; -.
DR MassIVE; Q96P64; -.
DR PaxDb; Q96P64; -.
DR PeptideAtlas; Q96P64; -.
DR PRIDE; Q96P64; -.
DR Antibodypedia; 58589; 81 antibodies from 16 providers.
DR DNASU; 119016; -.
DR Ensembl; ENST00000448048.7; ENSP00000392513.2; ENSG00000188234.15.
DR GeneID; 119016; -.
DR KEGG; hsa:119016; -.
DR UCSC; uc057szm.1; human.
DR CTD; 119016; -.
DR DisGeNET; 119016; -.
DR GeneCards; AGAP4; -.
DR HGNC; HGNC:23459; AGAP4.
DR HPA; ENSG00000188234; Low tissue specificity.
DR neXtProt; NX_Q96P64; -.
DR OpenTargets; ENSG00000188234; -.
DR PharmGKB; PA164715122; -.
DR VEuPathDB; HostDB:ENSG00000188234; -.
DR eggNOG; KOG0705; Eukaryota.
DR GeneTree; ENSGT00940000163475; -.
DR HOGENOM; CLU_007326_4_0_1; -.
DR InParanoid; Q96P64; -.
DR OMA; LNICEVH; -.
DR OrthoDB; 751525at2759; -.
DR PhylomeDB; Q96P64; -.
DR TreeFam; TF317762; -.
DR PathwayCommons; Q96P64; -.
DR SignaLink; Q96P64; -.
DR BioGRID-ORCS; 119016; 36 hits in 630 CRISPR screens.
DR ChiTaRS; AGAP4; human.
DR GenomeRNAi; 119016; -.
DR Pharos; Q96P64; Tdark.
DR PRO; PR:Q96P64; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q96P64; protein.
DR Bgee; ENSG00000188234; Expressed in cerebellar hemisphere and 97 other tissues.
DR ExpressionAtlas; Q96P64; baseline and differential.
DR Genevisible; Q96P64; HS.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR Gene3D; 1.10.220.150; -; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF01412; ArfGap; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00105; ArfGap; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50115; ARFGAP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW ANK repeat; GTPase activation; Metal-binding; Reference proteome; Repeat;
KW Zinc; Zinc-finger.
FT CHAIN 1..663
FT /note="Arf-GAP with GTPase, ANK repeat and PH domain-
FT containing protein 4"
FT /id="PRO_0000284673"
FT DOMAIN 259..420
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 441..561
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REPEAT 600..629
FT /note="ANK 1"
FT REPEAT 633..662
FT /note="ANK 2"
FT ZN_FING 456..479
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 185..216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 231..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..381
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 519..540
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..250
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 661
FT /note="K -> E (in dbSNP:rs15718)"
FT /evidence="ECO:0000269|Ref.1"
FT /id="VAR_031804"
FT CONFLICT 334
FT /note="T -> S (in Ref. 1; AAL10290)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 663 AA; 73070 MW; 49F11692C58577E2 CRC64;
MGNILTCRVH PSVSLEFDQQ QGSVCPSESE IYEAGAGDRM AGAPMAAAVQ PAEVTVEVGE
DLHMHHVRDR EMPEALEFNP SANPEASTIF QRNSQTDVVE IRRSNCTNHV STVRFSQQYS
LCSTIFLDDS TAIQHYLTMT IISVTLEIPH HITQRDADRS LSIPDEQLHS FAVSTVHIMK
KRNGGGSLNN YSSSIPSTPS TSQEDPQFSV PPTANTPTPV CKRSMRWSNL FTSEKGSDPD
KERKAPENHA DTIGSGRAIP IKQGMLLKRS GKWLKTWKKK YVTLCSNGVL TYYSSLGDYM
KNIHKKEIDL QTSTIKVPGK WPSLATSACT PISTSKSNGL SKDMDTGLGD SICFSPSISS
TTSPKLNPPP SPHANKKKHL KKKSTNNFMI VSATGQTWHF EATTYEERDA WVQAIQSQIL
ASLQSCESSK SKSQLTSQSK AMALQSIQNM RGNAHCVDCE TQNPKWASLN LGVLMCIECS
GIHRSLGTRL SRVRSLELDD WPVELRKVMS SIGNDLANSI WEGSSQGQTK PSEKSTREEK
ERWIRSKYEE KLFLAPLPCT ELSLGQQLLR ATADEDLQTA ILLLAHGSRE EVNETCGEGD
GCTALHLACR KGNVVLAQLL IWYGVDVMAR DAHGNTALTY ARQASSQECI NVLLQYGCPD
KCV
