ID   EMW1_YEAST              Reviewed;         904 AA.
AC   P42842; D6W0N2;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 162.
DE   RecName: Full=Essential for maintenance of the cell wall protein 1;
GN   Name=EMW1; OrderedLocusNames=YNL313C; ORFNames=N0364;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / FY1676;
RX   PubMed=7502583; DOI=10.1002/yea.320111109;
RA   Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.;
RT   "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV
RT   identifies six known genes, a new member of the hexose transporter family
RT   and ten new open reading frames.";
RL   Yeast 11:1077-1085(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   INDUCTION.
RX   PubMed=11206552; DOI=10.1038/35054095;
RA   Iyer V.R., Horak C.E., Scafe C.S., Botstein D., Snyder M., Brown P.O.;
RT   "Genomic binding sites of the yeast cell-cycle transcription factors SBF
RT   and MBF.";
RL   Nature 409:533-538(2001).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP   ANALYSIS].
RX   PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA   Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA   Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA   Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT   "Assigning function to yeast proteins by integration of technologies.";
RL   Mol. Cell 12:1353-1365(2003).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   FUNCTION.
RX   PubMed=21273246; DOI=10.1099/mic.0.045971-0;
RA   Sipling T., Zhai C., Panaretou B.;
RT   "Emw1p/YNL313cp is essential for maintenance of the cell wall in
RT   Saccharomyces cerevisiae.";
RL   Microbiology 157:1032-1041(2011).
CC   -!- FUNCTION: Required for the maintenance of the cell wall integrity.
CC       {ECO:0000269|PubMed:21273246}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14690591}. Nucleus
CC       {ECO:0000269|PubMed:14690591}.
CC   -!- INDUCTION: Target of SBF and MBF transcription factor complexes that
CC       activate gene expression during the G1/S transition of the cell cycle.
CC       {ECO:0000269|PubMed:11206552}.
CC   -!- MISCELLANEOUS: Present with 8070 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the TTC27 family. {ECO:0000305}.
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DR   EMBL; Z46259; CAA86383.1; -; Genomic_DNA.
DR   EMBL; Z71588; CAA96242.1; -; Genomic_DNA.
DR   EMBL; Z71589; CAA96243.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10248.1; -; Genomic_DNA.
DR   PIR; S51299; S51299.
DR   RefSeq; NP_014086.1; NM_001183151.1.
DR   AlphaFoldDB; P42842; -.
DR   SMR; P42842; -.
DR   BioGRID; 35526; 58.
DR   DIP; DIP-6783N; -.
DR   IntAct; P42842; 20.
DR   MINT; P42842; -.
DR   STRING; 4932.YNL313C; -.
DR   iPTMnet; P42842; -.
DR   MaxQB; P42842; -.
DR   PaxDb; P42842; -.
DR   PRIDE; P42842; -.
DR   EnsemblFungi; YNL313C_mRNA; YNL313C; YNL313C.
DR   GeneID; 855403; -.
DR   KEGG; sce:YNL313C; -.
DR   SGD; S000005257; EMW1.
DR   VEuPathDB; FungiDB:YNL313C; -.
DR   eggNOG; KOG1128; Eukaryota.
DR   GeneTree; ENSGT00500000044929; -.
DR   HOGENOM; CLU_004905_0_1_1; -.
DR   InParanoid; P42842; -.
DR   OMA; DARIWDN; -.
DR   BioCyc; YEAST:G3O-33300-MON; -.
DR   PRO; PR:P42842; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P42842; protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   InterPro; IPR044244; TTC27/Emw1.
DR   PANTHER; PTHR16193; PTHR16193; 1.
DR   SMART; SM00028; TPR; 3.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS50005; TPR; 2.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   1: Evidence at protein level;
KW   Cell wall biogenesis/degradation; Cytoplasm; Nucleus; Reference proteome;
KW   Repeat; TPR repeat.
FT   CHAIN           1..904
FT                   /note="Essential for maintenance of the cell wall protein
FT                   1"
FT                   /id="PRO_0000203365"
FT   REPEAT          510..544
FT                   /note="TPR 1"
FT   REPEAT          563..596
FT                   /note="TPR 2"
FT   REPEAT          603..636
FT                   /note="TPR 3"
FT   REPEAT          637..670
FT                   /note="TPR 4"
FT   REPEAT          671..704
FT                   /note="TPR 5"
FT   REPEAT          706..739
FT                   /note="TPR 6"
SQ   SEQUENCE   904 AA;  102336 MW;  7170398FA95A0BD8 CRC64;
     METLLHAKLL LSAEVESLKS GSFDQTYVKK AEHIISGESY QLVQQFVDKF KGKISISGEI
     STSSVIAALN DFLNVEVFKM GQENEMLFLA IALLQTFIQN NYTGPAARLK AISGLFGKTG
     IEIGAVNTAL SRSLAIMGQP AYEFMDDPLY LVLSLLLLER ITGQKSLFDV TPDQEIPLPI
     ISAESTPGLL AVAYWWWARA LLTQLSLIPE PSGFQASVAS AIYQSADLAY AITKELPESI
     HEDFKRELCA MYYLENVKCS LAINTEHLCL PSLTRAKKIT NFEFVMTGAR ATRTKYQQKA
     HAGLIILAKS FTFQNFALRT TSATPETFAL ESDLLLEKPH FESIADEPLD EQIYSKRQKV
     DLNEGYEEDK LLPLALRQEN IPKLLLDLNP NDQPTLSDYD NIQLLLRLYT IKNTTPAKDP
     LVEEELTALL SRILYQNGDK NWSIFARSLW ERSIIETTKA KTIERGLLQM QSLVEELDLK
     IKSKLVPSSS EINVASRLSY IHQLPFIPRW QLDATLAEKY MSLGILKSAV EIYERLGMAC
     ETALCYAAVG DEKKAEEILL QRINENDSDA RAYSILGDIK QDPSLWEKSW EIGKYVNAKN
     SLAKYYFNPP PKSGAQPNYS ATLKHLNDSL RQYPLSFETW YFYGCVGLQC GKMQIAAEAF
     TRCVSLDPYH ALSWSNLSAA YTKMDKLKEA YSCLKRAISC DAQKNWKIWE NYMLVAVKLN
     EWEDVLTACK QLVSIRRDKS GEGSIDLPII EKLVELLVTS EYPEEPQQLS YFQKSCTEFI
     CNTLPQVITT SARCWRLVAR VELWRKRPWA ALECHEKAYR AISHNPDLEV EEKVWNDTVD
     ACEDLVAAYE SLGEMEGKYG PGSLVCKDWK YKCRSTIKAL MSKGKGRWDD SPGWDRLVEA
     RSQI