EMX2_MOUSE
ID EMX2_MOUSE Reviewed; 253 AA.
AC Q04744; G3X9A6; Q80UE7;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 3.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Homeobox protein EMX2;
DE AltName: Full=Empty spiracles homolog 2;
DE AltName: Full=Empty spiracles-like protein 2;
GN Name=Emx2; Synonyms=Emx-2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=FVB/N;
RX PubMed=12573261; DOI=10.1016/s0888-7543(02)00023-x;
RA Noonan F.C., Goodfellow P.J., Staloch L.J., Mutch D.G., Simon T.C.;
RT "Antisense transcripts at the EMX2 locus in human and mouse.";
RL Genomics 81:58-66(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 155-220, FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=1352754; DOI=10.1002/j.1460-2075.1992.tb05319.x;
RA Simeone A., Gulisano M., Acampora D., Stornaiuolo A., Rambaldi M.,
RA Boncinelli E.;
RT "Two vertebrate homeobox genes related to the Drosophila empty spiracles
RT gene are expressed in the embryonic cerebral cortex.";
RL EMBO J. 11:2541-2550(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 155-220.
RC TISSUE=Brain;
RX PubMed=1353865; DOI=10.1038/358687a0;
RA Simeone A., Acampora D., Gulisano M., Stornaiuolo A., Boncinelli E.;
RT "Nested expression domains of four homeobox genes in developing rostral
RT brain.";
RL Nature 358:687-690(1992).
RN [6]
RP FUNCTION.
RX PubMed=15147765; DOI=10.1016/j.mod.2004.03.013;
RA Shinozaki K., Yoshida M., Nakamura M., Aizawa S., Suda Y.;
RT "Emx1 and Emx2 cooperate in initial phase of archipallium development.";
RL Mech. Dev. 121:475-489(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Transcription factor, which in cooperation with EMX2, acts to
CC generate the boundary between the roof and archipallium in the
CC developing brain. May function in combinations with OTX1/2 to specify
CC cell fates in the developing central nervous system.
CC {ECO:0000269|PubMed:1352754, ECO:0000269|PubMed:15147765}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Cerebral cortex. {ECO:0000269|PubMed:1352754}.
CC -!- SIMILARITY: Belongs to the EMX homeobox family. {ECO:0000305}.
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DR EMBL; AY117415; AAM94018.1; -; mRNA.
DR EMBL; AC098733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466585; EDL01826.1; -; Genomic_DNA.
DR EMBL; X68882; CAA48753.1; -; mRNA.
DR CCDS; CCDS29937.1; -.
DR PIR; S29086; S29086.
DR RefSeq; NP_034262.2; NM_010132.2.
DR AlphaFoldDB; Q04744; -.
DR SMR; Q04744; -.
DR BioGRID; 199443; 4.
DR IntAct; Q04744; 4.
DR STRING; 10090.ENSMUSP00000053361; -.
DR iPTMnet; Q04744; -.
DR PhosphoSitePlus; Q04744; -.
DR PaxDb; Q04744; -.
DR PeptideAtlas; Q04744; -.
DR PRIDE; Q04744; -.
DR ProteomicsDB; 275750; -.
DR Antibodypedia; 18764; 342 antibodies from 32 providers.
DR DNASU; 13797; -.
DR Ensembl; ENSMUST00000062216; ENSMUSP00000053361; ENSMUSG00000043969.
DR GeneID; 13797; -.
DR KEGG; mmu:13797; -.
DR UCSC; uc008ibl.1; mouse.
DR CTD; 2018; -.
DR MGI; MGI:95388; Emx2.
DR VEuPathDB; HostDB:ENSMUSG00000043969; -.
DR eggNOG; KOG0843; Eukaryota.
DR GeneTree; ENSGT00940000157425; -.
DR HOGENOM; CLU_049668_1_0_1; -.
DR InParanoid; Q04744; -.
DR OMA; RWRMETQ; -.
DR OrthoDB; 1189098at2759; -.
DR PhylomeDB; Q04744; -.
DR TreeFam; TF317015; -.
DR BioGRID-ORCS; 13797; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Emx2; mouse.
DR PRO; PR:Q04744; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q04744; protein.
DR Bgee; ENSMUSG00000043969; Expressed in fallopian tube and 183 other tissues.
DR ExpressionAtlas; Q04744; baseline and differential.
DR Genevisible; Q04744; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR GO; GO:0007420; P:brain development; IMP:MGI.
DR GO; GO:0021846; P:cell proliferation in forebrain; IMP:MGI.
DR GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR GO; GO:0021987; P:cerebral cortex development; IGI:MGI.
DR GO; GO:0021796; P:cerebral cortex regionalization; IMP:MGI.
DR GO; GO:0021542; P:dentate gyrus development; IMP:MGI.
DR GO; GO:0021885; P:forebrain cell migration; IMP:MGI.
DR GO; GO:0030900; P:forebrain development; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR GO; GO:0001764; P:neuron migration; IMP:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IDA:MGI.
DR GO; GO:0072197; P:ureter morphogenesis; IGI:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..253
FT /note="Homeobox protein EMX2"
FT /id="PRO_0000048869"
FT DNA_BIND 155..214
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 213..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..228
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 103
FT /note="S -> L (in Ref. 1; AAM94018)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 253 AA; 28374 MW; 4AE978446786B204 CRC64;
MFQPAPKRCF TIESLVAKDS PLPASRSEDP IRPAALSYAN SSPINPFLNG FHSAAAAAAA
GRGVYSNPDL VFAEAVSHPP NPAVPVHPVP PPHALAAHPL PSSHSPHPLF ASQQRDPSTF
YPWLIHRYRY LGHRFQGNDT SPESFLLHNA LARKPKRIRT AFSPSQLLRL EHAFEKNHYV
VGAERKQLAH SLSLTETQVK VWFQNRRTKF KRQKLEEEGS DSQQKKKGTH HINRWRIATK
QASPEEIDVT SDD
