ID   ENA2_HORSE              Reviewed;          46 AA.
AC   P56928;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Antimicrobial peptide eNAP-2;
DE   Flags: Fragment;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   PROTEIN SEQUENCE, AND ANTIBACTERIAL ACTIVITY.
RC   TISSUE=Neutrophil;
RX   PubMed=1452336; DOI=10.1128/iai.60.12.5042-5047.1992;
RA   Couto M.A., Harwig S.S.L., Cullor J.S., Hughes J.P., Lehrer R.I.;
RT   "eNAP-2, a novel cysteine-rich bactericidal peptide from equine
RT   leukocytes.";
RL   Infect. Immun. 60:5042-5047(1992).
RN   [2]
RP   ANTIPROTEASE ACTIVITY.
RX   PubMed=8514405; DOI=10.1128/iai.61.7.2991-2994.1993;
RA   Couto M.A., Harwig S.S.L., Lehrer R.I.;
RT   "Selective inhibition of microbial serine proteases by eNAP-2, an
RT   antimicrobial peptide from equine neutrophils.";
RL   Infect. Immun. 61:2991-2994(1993).
CC   -!- FUNCTION: Has antibiotic activity against several equine uterine
CC       pathogens; S.zooepidemicus, E.coli and P.aeruginosa. Highly efficient
CC       against S.zoopedemicus. Not active against K.pneumoniae. Selectively
CC       inactivates microbial serine proteases (subtilisin A and proteinase K)
CC       without inhibiting mammalian serine proteases (human neutrophil
CC       elastase, human cathepsin G and bovine pancreatic trypsin).
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DR   PIR; A49212; A49212.
DR   AlphaFoldDB; P56928; -.
DR   SMR; P56928; -.
DR   Proteomes; UP000002281; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   InterPro; IPR036645; Elafin-like_sf.
DR   SUPFAM; SSF57256; SSF57256; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Protease inhibitor;
KW   Reference proteome.
FT   CHAIN           1..>46
FT                   /note="Antimicrobial peptide eNAP-2"
FT                   /id="PRO_0000188987"
FT   DOMAIN          12..>46
FT                   /note="WAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT   NON_TER         46
SQ   SEQUENCE   46 AA;  4769 MW;  8C30936DA38A7AEC CRC64;
     EVERKHPLGG SRPGRCPTVP PGTFGHCACL CTGDASEPKG QKCCSN