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ENB1_YEAST
ID   ENB1_YEAST              Reviewed;         606 AA.
AC   Q08299; D6W1R2;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 149.
DE   RecName: Full=Siderophore iron transporter ENB1;
DE   AltName: Full=Enterobactin permease;
DE   AltName: Full=Siderophore iron transporter ARN4;
GN   Name=ENB1; Synonyms=ARN4; OrderedLocusNames=YOL158C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10744769; DOI=10.1074/jbc.275.14.10709;
RA   Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D.,
RA   Kaplan J., Philpott C.C.;
RT   "Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence
RT   for two pathways of iron uptake.";
RL   J. Biol. Chem. 275:10709-10715(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA   Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA   Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA   Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA   Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA   Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA   Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA   Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA   Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA   Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA   Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA   Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA   Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION.
RX   PubMed=12196168; DOI=10.1042/bst0300698;
RA   Philpott C.C., Protchenko O., Kim Y.W., Boretsky Y., Shakoury-Elizeh M.;
RT   "The response to iron deprivation in Saccharomyces cerevisiae: expression
RT   of siderophore-based systems of iron uptake.";
RL   Biochem. Soc. Trans. 30:698-702(2002).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Involved in the transport of siderophore enterobactin and so
CC       has a role in iron homeostasis. {ECO:0000269|PubMed:12196168}.
CC   -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; Z74900; CAA99180.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA10628.1; -; Genomic_DNA.
DR   PIR; S66857; S66857.
DR   RefSeq; NP_014484.1; NM_001183411.1.
DR   AlphaFoldDB; Q08299; -.
DR   BioGRID; 34260; 48.
DR   STRING; 4932.YOL158C; -.
DR   TCDB; 2.A.1.16.2; the major facilitator superfamily (mfs).
DR   iPTMnet; Q08299; -.
DR   MaxQB; Q08299; -.
DR   PaxDb; Q08299; -.
DR   PRIDE; Q08299; -.
DR   EnsemblFungi; YOL158C_mRNA; YOL158C; YOL158C.
DR   GeneID; 854007; -.
DR   KEGG; sce:YOL158C; -.
DR   SGD; S000005518; ENB1.
DR   VEuPathDB; FungiDB:YOL158C; -.
DR   eggNOG; KOG0254; Eukaryota.
DR   HOGENOM; CLU_012970_2_1_1; -.
DR   InParanoid; Q08299; -.
DR   OMA; MSVYQIG; -.
DR   BioCyc; YEAST:G3O-33546-MON; -.
DR   PRO; PR:Q08299; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   RNAct; Q08299; protein.
DR   GO; GO:0071944; C:cell periphery; HDA:SGD.
DR   GO; GO:0033101; C:cellular bud membrane; IDA:SGD.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR   GO; GO:0015620; F:ferric-enterobactin transmembrane transporter activity; IMP:SGD.
DR   GO; GO:0015343; F:siderophore transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IBA:GO_Central.
DR   GO; GO:0015685; P:ferric-enterobactin import into cell; IMP:SGD.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Endosome; Ion transport; Iron; Iron transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..606
FT                   /note="Siderophore iron transporter ENB1"
FT                   /id="PRO_0000084865"
FT   TOPO_DOM        1..140
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        162..187
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        188..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        209..224
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        246..279
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        280..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        301..310
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        311..331
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        332..337
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        338..358
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        359..360
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        361..381
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        382..390
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        391..410
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        411..414
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        415..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        433..435
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        436..456
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        457..480
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        481..501
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        502..550
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        551..571
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        572..606
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   606 AA;  66970 MW;  4ED81A2A6A43815D CRC64;
     MLETDHSRND NLDDKSTVCY SEKTDSNVEK STTSGLRRID AVNKVLSDYS SFTAFGVTFS
     SLKTALLVAL FLQGYCTGLG GQISQSIQTY AANSFGKHSQ VGSINTVKSI VASVVAVPYA
     RISDRFGRIE CWIFALVLYT IGEIISAATP TFSGLFAGIV IQQFGYSGFR LLATALTGDL
     SGLRDRTFAM NIFLIPVIIN TWVSGNIVSS VAGNVAPYKW RWGYGIFCII VPISTLILVL
     PYVYAQYISW RSGKLPPLKL KEKGQTLRQT LWKFADDINL IGVILFTAFL VLVLLPLTIA
     GGATSKWREG HIIAMIVVGG CLGFIFLIWE LKFAKNPFIP RVYLGDPTIY VALLMEFVWR
     LGLQIELEYL VTVLMVAFGE STLSAQRIAQ LYNFLQSCTN IVVGIMLHFY PHPKVFVVAG
     SLLGVIGMGL LYKYRVVYDG ISGLIGAEIV VGIAGGMIRF PMWTLVHAST THNEMATVTG
     LLMSVYQIGD AVGASIAGAI WTQRLAKELI QRLGSSLGMA IYKSPLNYLK KYPIGSEVRV
     QMIESYSKIQ RLLIIVSISF AAFNAVLCFF LRGFTVNKKQ SLSAEEREKE KLKIKQQSWL
     RRVIGY
 
 
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