ID   ENCAP_BRELN             Reviewed;         266 AA.
AC   Q45296; A0A2H1I3X5;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Type 1 encapsulin shell protein;
DE   AltName: Full=Linocin M18 {ECO:0000303|PubMed:7986050};
DE   AltName: Full=M18 encapsulin {ECO:0000303|PubMed:19172747};
GN   Name=enc {ECO:0000305}; Synonyms=lin {ECO:0000303|PubMed:8919789};
GN   ORFNames=BLIN101_00808 {ECO:0000312|EMBL:SMX69863.1},
GN   CXR29_00100 {ECO:0000312|EMBL:AZT99314.1};
OS   Brevibacterium linens.
OC   Bacteria; Actinobacteria; Micrococcales; Brevibacteriaceae; Brevibacterium.
OX   NCBI_TaxID=1703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M18;
RX   PubMed=8919789; DOI=10.1128/aem.62.4.1283-1286.1996;
RA   Valdes-Stauber N., Scherer S.;
RT   "Nucleotide sequence and taxonomical distribution of the bacteriocin gene
RT   lin cloned from Brevibacterium linens M18.";
RL   Appl. Environ. Microbiol. 62:1283-1286(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu101;
RA   Monnet C.;
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19391;
RX   PubMed=31244798; DOI=10.3389/fmicb.2019.01270;
RA   Levesque S., de Melo A.G., Labrie S.J., Moineau S.;
RT   "Mobilome of Brevibacterium aurantiacum Sheds Light on Its Genetic
RT   Diversity and Its Adaptation to Smear-Ripened Cheeses.";
RL   Front. Microbiol. 10:1270-1270(2019).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-19, FUNCTION AS A BACTERIOCIN, SUBUNIT, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=M18;
RX   PubMed=7986050; DOI=10.1128/aem.60.10.3809-3814.1994;
RA   Valdes-Stauber N., Scherer S.;
RT   "Isolation and characterization of Linocin M18, a bacteriocin produced by
RT   Brevibacterium linens.";
RL   Appl. Environ. Microbiol. 60:3809-3814(1994).
RN   [5]
RP   FUNCTION, NOT A BACTERIOCIN, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=M18;
RX   PubMed=19172747; DOI=10.1038/nsmb.1473;
RA   Sutter M., Boehringer D., Gutmann S., Gunther S., Prangishvili D.,
RA   Loessner M.J., Stetter K.O., Weber-Ban E., Ban N.;
RT   "Structural basis of enzyme encapsulation into a bacterial
RT   nanocompartment.";
RL   Nat. Struct. Mol. Biol. 15:939-947(2008).
RN   [6]
RP   PUTATIVE FUNCTION, SUBUNIT, PUTATIVE SUBCELLULAR LOCATION, AND
RP   CLASSIFICATION.
RX   PubMed=34362927; DOI=10.1038/s41467-021-25071-y;
RA   Andreas M.P., Giessen T.W.;
RT   "Large-scale computational discovery and analysis of virus-derived
RT   microbial nanocompartments.";
RL   Nat. Commun. 12:4748-4748(2021).
CC   -!- FUNCTION: Shell component of a type 1 encapsulin nanocompartment.
CC       Assembles into proteinaceous shells 23-24 nm in diameter with 2-2.5 nm
CC       thick walls. Cargo protein DyP (dye-decolorizing peroxidase) is
CC       targeted to the interior via its C-terminal extension; probably only 1
CC       DyP hexamer is incorporated into each shell. Empty shells can be
CC       isolated in the absence of cargo. {ECO:0000269|PubMed:19172747}.
CC   -!- SUBUNIT: Homomultimeric (PubMed:7986050). This encapsulin
CC       nanocompartment is formed by 60 subunits; monomers form pentamers,
CC       which assemble to form hollow shells with several openings (By
CC       similarity). {ECO:0000250|UniProtKB:Q9WZP2,
CC       ECO:0000269|PubMed:7986050}.
CC   -!- SUBCELLULAR LOCATION: Encapsulin nanocompartment
CC       {ECO:0000269|PubMed:19172747, ECO:0000269|PubMed:7986050}.
CC   -!- SIMILARITY: Belongs to the encapsulin family. Family 1 subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a bacteriocin (PubMed:7986050);
CC       it has since been shown this is not the case (PubMed:19172747).
CC       {ECO:0000269|PubMed:19172747, ECO:0000269|PubMed:7986050}.
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DR   EMBL; X93588; CAA63787.1; -; Genomic_DNA.
DR   EMBL; CP026734; AZT99314.1; -; Genomic_DNA.
DR   EMBL; FXZA01000002; SMX69863.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q45296; -.
DR   SMR; Q45296; -.
DR   IntAct; Q45296; 1.
DR   MEROPS; U56.001; -.
DR   EnsemblBacteria; AZT99314; AZT99314; CXR29_00100.
DR   EnsemblBacteria; SMX69863; SMX69863; BLIN101_00808.
DR   Proteomes; UP000234498; Unassembled WGS sequence.
DR   Proteomes; UP000282883; Chromosome.
DR   GO; GO:0140737; C:encapsulin nanocompartment; IDA:UniProtKB.
DR   InterPro; IPR007544; Linocin_M18.
DR   Pfam; PF04454; Linocin_M18; 1.
DR   PIRSF; PIRSF019254; CFP29; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Encapsulin nanocompartment.
FT   CHAIN           1..266
FT                   /note="Type 1 encapsulin shell protein"
FT                   /id="PRO_0000343953"
FT   CONFLICT        16
FT                   /note="W -> A (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   266 AA;  28597 MW;  C33D960AC4C0529E CRC64;
     MNNLYRELAP IPGPAWAEIE EEARRTFKRN IAGRRIVDVA GPTGFETSAV TTGHIRDVQS
     ETSGLQVKQR IVQEYIELRT PFTVTRQAID DVARGSGDSD WQPVKDAATT IAMAEDRAIL
     HGLDAAGIGG IVPGSSNAAV AIPDAVEDFA DAVAQALSVL RTVGVDGPYS LLLSSAEYTK
     VSESTDHGYP IREHLSRQLG AGEIIWAPAL EGALLVSTRG GDYELHLGQD LSIGYYSHDS
     ETVELYLQET FGFLALTDES SVPLSL