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ENCB_MYXXD
ID   ENCB_MYXXD              Reviewed;         158 AA.
AC   Q1D6H3;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Encapsulin nanocompartment cargo protein EncB {ECO:0000303|PubMed:25024436};
GN   Name=encB {ECO:0000303|PubMed:25024436}; ORFNames=MXAN_3557;
OS   Myxococcus xanthus (strain DK1622).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=246197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DK1622;
RX   PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA   Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA   Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA   Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA   Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT   "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=DZ2;
RX   PubMed=19996678; DOI=10.4014/jmb.0903.0112;
RA   Kim D., Chung J., Hyun H., Lee C., Lee K., Cho K.;
RT   "Operon required for fruiting body development in Myxococcus xanthus.";
RL   J. Microbiol. Biotechnol. 19:1288-1294(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SEQUENCE REVISION TO N-TERMINUS,
RP   FUNCTION, SUBCELLULAR LOCATION, AND DOMAIN.
RC   STRAIN=DK1622;
RX   PubMed=25024436; DOI=10.15252/embj.201488566;
RA   McHugh C.A., Fontana J., Nemecek D., Cheng N., Aksyuk A.A., Heymann J.B.,
RA   Winkler D.C., Lam A.S., Wall J.S., Steven A.C., Hoiczyk E.;
RT   "A virus capsid-like nanocompartment that stores iron and protects bacteria
RT   from oxidative stress.";
RL   EMBO J. 33:1896-1911(2014).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC   STRAIN=DK1622;
RX   PubMed=31194509; DOI=10.1021/acsnano.9b03140;
RA   Sigmund F., Pettinger S., Kube M., Schneider F., Schifferer M.,
RA   Schneider S., Efremova M.V., Pujol-Marti J., Aichler M., Walch A.,
RA   Misgeld T., Dietz H., Westmeyer G.G.;
RT   "Iron-Sequestering Nanocompartments as Multiplexed Electron Microscopy Gene
RT   Reporters.";
RL   ACS Nano 13:8114-8123(2019).
CC   -!- FUNCTION: Cargo protein of a type 1 encapsulin nanocompartment. May
CC       help nucleate Fe atoms in the interior of the encapsulin
CC       nanocompartment (Probable). Present in about 36 copies/encapsulin
CC       nanocompartment (PubMed:25024436). {ECO:0000269|PubMed:25024436,
CC       ECO:0000305|PubMed:25024436}.
CC   -!- SUBCELLULAR LOCATION: Encapsulin nanocompartment
CC       {ECO:0000269|PubMed:25024436}. Note=Probably lies against the interior
CC       face of the nanocompartment. {ECO:0000305|PubMed:25024436}.
CC   -!- DISRUPTION PHENOTYPE: Forms wild-type fruiting bodies.
CC       {ECO:0000269|PubMed:19996678}.
CC   -!- BIOTECHNOLOGY: The encapsulin and a cargo construct (an encB-encC-encD
CC       fusion) can be overexpressed in E.coli and in human HEK293T cells. In
CC       HEK293T in the presence of 0.5 M ferrous ammonium sulfate
CC       nanocompartments can be detected and used as cell markers. Coexpression
CC       of this nanocompartment with a larger nanocompartment from
CC       Q.thermotolerans (AC A0A0F5HPP7) allows of expression of different
CC       sized iron-rich particles. The encapsulin shell proteins are not seen
CC       to mix. {ECO:0000269|PubMed:31194509}.
CC   -!- SIMILARITY: Belongs to the ferritin-like superfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABF88760.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000269|PubMed:25024436};
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DR   EMBL; CP000113; ABF88760.1; ALT_INIT; Genomic_DNA.
DR   PDB; 7S5C; X-ray; 1.86 A; A/B/C/D/E/F/G/H/I/J=1-100.
DR   PDB; 7S5K; X-ray; 1.95 A; A/B/C/D/E/F/G/H/I/J=14-81.
DR   PDBsum; 7S5C; -.
DR   PDBsum; 7S5K; -.
DR   SMR; Q1D6H3; -.
DR   IntAct; Q1D6H3; 1.
DR   MINT; Q1D6H3; -.
DR   STRING; 246197.MXAN_3557; -.
DR   EnsemblBacteria; ABF88760; ABF88760; MXAN_3557.
DR   KEGG; mxa:MXAN_3557; -.
DR   eggNOG; COG1633; Bacteria.
DR   HOGENOM; CLU_1676786_0_0_7; -.
DR   OMA; INEYEAY; -.
DR   Proteomes; UP000002402; Chromosome.
DR   GO; GO:0140737; C:encapsulin nanocompartment; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IEA:UniProtKB-KW.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   SUPFAM; SSF47240; SSF47240; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Encapsulin nanocompartment; Iron; Iron storage;
KW   Metal-binding; Reference proteome.
FT   CHAIN           1..158
FT                   /note="Encapsulin nanocompartment cargo protein EncB"
FT                   /id="PRO_0000455321"
FT   REGION          92..158
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          142..149
FT                   /note="Probable targeting peptide"
FT                   /evidence="ECO:0000305|PubMed:25024436"
FT   MOTIF           52..55
FT                   /note="Di-iron-binding motif"
FT                   /evidence="ECO:0000305|PubMed:25024436"
FT   MOTIF           58..61
FT                   /note="Di-iron-binding motif"
FT                   /evidence="ECO:0000305|PubMed:25024436"
FT   BINDING         22
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT   BINDING         22
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT   BINDING         52
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT   BINDING         52
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT   BINDING         55
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT   BINDING         55
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
SQ   SEQUENCE   158 AA;  16856 MW;  BE08971102EEC76E CRC64;
     MAGPPDSDLD DVARIRLVLA RELETINEYE AYARASSNPE VRAFFQHLAA EEKEHVSEAV
     HMLRMLDSGQ NDHFAKPFVP GHFQAAEAPA PATVHVPTPD GPAFSVNGRN GRLPSEPPTS
     LPPQRLLYGL PAPPPAVESH PLTVGSLRRG GGGSGSGR
 
 
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