ENCB_MYXXD
ID ENCB_MYXXD Reviewed; 158 AA.
AC Q1D6H3;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Encapsulin nanocompartment cargo protein EncB {ECO:0000303|PubMed:25024436};
GN Name=encB {ECO:0000303|PubMed:25024436}; ORFNames=MXAN_3557;
OS Myxococcus xanthus (strain DK1622).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC Cystobacterineae; Myxococcaceae; Myxococcus.
OX NCBI_TaxID=246197;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DK1622;
RX PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN [2]
RP DISRUPTION PHENOTYPE.
RC STRAIN=DZ2;
RX PubMed=19996678; DOI=10.4014/jmb.0903.0112;
RA Kim D., Chung J., Hyun H., Lee C., Lee K., Cho K.;
RT "Operon required for fruiting body development in Myxococcus xanthus.";
RL J. Microbiol. Biotechnol. 19:1288-1294(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, SEQUENCE REVISION TO N-TERMINUS,
RP FUNCTION, SUBCELLULAR LOCATION, AND DOMAIN.
RC STRAIN=DK1622;
RX PubMed=25024436; DOI=10.15252/embj.201488566;
RA McHugh C.A., Fontana J., Nemecek D., Cheng N., Aksyuk A.A., Heymann J.B.,
RA Winkler D.C., Lam A.S., Wall J.S., Steven A.C., Hoiczyk E.;
RT "A virus capsid-like nanocompartment that stores iron and protects bacteria
RT from oxidative stress.";
RL EMBO J. 33:1896-1911(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC STRAIN=DK1622;
RX PubMed=31194509; DOI=10.1021/acsnano.9b03140;
RA Sigmund F., Pettinger S., Kube M., Schneider F., Schifferer M.,
RA Schneider S., Efremova M.V., Pujol-Marti J., Aichler M., Walch A.,
RA Misgeld T., Dietz H., Westmeyer G.G.;
RT "Iron-Sequestering Nanocompartments as Multiplexed Electron Microscopy Gene
RT Reporters.";
RL ACS Nano 13:8114-8123(2019).
CC -!- FUNCTION: Cargo protein of a type 1 encapsulin nanocompartment. May
CC help nucleate Fe atoms in the interior of the encapsulin
CC nanocompartment (Probable). Present in about 36 copies/encapsulin
CC nanocompartment (PubMed:25024436). {ECO:0000269|PubMed:25024436,
CC ECO:0000305|PubMed:25024436}.
CC -!- SUBCELLULAR LOCATION: Encapsulin nanocompartment
CC {ECO:0000269|PubMed:25024436}. Note=Probably lies against the interior
CC face of the nanocompartment. {ECO:0000305|PubMed:25024436}.
CC -!- DISRUPTION PHENOTYPE: Forms wild-type fruiting bodies.
CC {ECO:0000269|PubMed:19996678}.
CC -!- BIOTECHNOLOGY: The encapsulin and a cargo construct (an encB-encC-encD
CC fusion) can be overexpressed in E.coli and in human HEK293T cells. In
CC HEK293T in the presence of 0.5 M ferrous ammonium sulfate
CC nanocompartments can be detected and used as cell markers. Coexpression
CC of this nanocompartment with a larger nanocompartment from
CC Q.thermotolerans (AC A0A0F5HPP7) allows of expression of different
CC sized iron-rich particles. The encapsulin shell proteins are not seen
CC to mix. {ECO:0000269|PubMed:31194509}.
CC -!- SIMILARITY: Belongs to the ferritin-like superfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF88760.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000269|PubMed:25024436};
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DR EMBL; CP000113; ABF88760.1; ALT_INIT; Genomic_DNA.
DR PDB; 7S5C; X-ray; 1.86 A; A/B/C/D/E/F/G/H/I/J=1-100.
DR PDB; 7S5K; X-ray; 1.95 A; A/B/C/D/E/F/G/H/I/J=14-81.
DR PDBsum; 7S5C; -.
DR PDBsum; 7S5K; -.
DR SMR; Q1D6H3; -.
DR IntAct; Q1D6H3; 1.
DR MINT; Q1D6H3; -.
DR STRING; 246197.MXAN_3557; -.
DR EnsemblBacteria; ABF88760; ABF88760; MXAN_3557.
DR KEGG; mxa:MXAN_3557; -.
DR eggNOG; COG1633; Bacteria.
DR HOGENOM; CLU_1676786_0_0_7; -.
DR OMA; INEYEAY; -.
DR Proteomes; UP000002402; Chromosome.
DR GO; GO:0140737; C:encapsulin nanocompartment; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IEA:UniProtKB-KW.
DR InterPro; IPR009078; Ferritin-like_SF.
DR SUPFAM; SSF47240; SSF47240; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Encapsulin nanocompartment; Iron; Iron storage;
KW Metal-binding; Reference proteome.
FT CHAIN 1..158
FT /note="Encapsulin nanocompartment cargo protein EncB"
FT /id="PRO_0000455321"
FT REGION 92..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..149
FT /note="Probable targeting peptide"
FT /evidence="ECO:0000305|PubMed:25024436"
FT MOTIF 52..55
FT /note="Di-iron-binding motif"
FT /evidence="ECO:0000305|PubMed:25024436"
FT MOTIF 58..61
FT /note="Di-iron-binding motif"
FT /evidence="ECO:0000305|PubMed:25024436"
FT BINDING 22
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 22
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 52
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 52
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 55
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 55
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
SQ SEQUENCE 158 AA; 16856 MW; BE08971102EEC76E CRC64;
MAGPPDSDLD DVARIRLVLA RELETINEYE AYARASSNPE VRAFFQHLAA EEKEHVSEAV
HMLRMLDSGQ NDHFAKPFVP GHFQAAEAPA PATVHVPTPD GPAFSVNGRN GRLPSEPPTS
LPPQRLLYGL PAPPPAVESH PLTVGSLRRG GGGSGSGR