ENCC_MYXXD
ID ENCC_MYXXD Reviewed; 130 AA.
AC Q1D3Y8;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Encapsulin nanocompartment cargo protein EncC {ECO:0000303|PubMed:25024436};
GN Name=encC {ECO:0000303|PubMed:25024436}; ORFNames=MXAN_4464;
OS Myxococcus xanthus (strain DK1622).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC Cystobacterineae; Myxococcaceae; Myxococcus.
OX NCBI_TaxID=246197;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DK1622;
RX PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, SEQUENCE REVISION TO N-TERMINUS,
RP FUNCTION, SUBCELLULAR LOCATION, AND DOMAIN.
RC STRAIN=DK1622;
RX PubMed=25024436; DOI=10.15252/embj.201488566;
RA McHugh C.A., Fontana J., Nemecek D., Cheng N., Aksyuk A.A., Heymann J.B.,
RA Winkler D.C., Lam A.S., Wall J.S., Steven A.C., Hoiczyk E.;
RT "A virus capsid-like nanocompartment that stores iron and protects bacteria
RT from oxidative stress.";
RL EMBO J. 33:1896-1911(2014).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC STRAIN=DK1622;
RX PubMed=31194509; DOI=10.1021/acsnano.9b03140;
RA Sigmund F., Pettinger S., Kube M., Schneider F., Schifferer M.,
RA Schneider S., Efremova M.V., Pujol-Marti J., Aichler M., Walch A.,
RA Misgeld T., Dietz H., Westmeyer G.G.;
RT "Iron-Sequestering Nanocompartments as Multiplexed Electron Microscopy Gene
RT Reporters.";
RL ACS Nano 13:8114-8123(2019).
CC -!- FUNCTION: Cargo protein of a type 1 encapsulin nanocompartment. May
CC help nucleate Fe atoms in the interior of the encapsulin
CC nanocompartment (Probable). Present in about 92 copies/encapsulin
CC nanocompartment (PubMed:25024436). {ECO:0000269|PubMed:25024436,
CC ECO:0000305|PubMed:25024436}.
CC -!- SUBCELLULAR LOCATION: Encapsulin nanocompartment
CC {ECO:0000269|PubMed:25024436}. Note=Probably lies against the interior
CC face of the nanocompartment. {ECO:0000305|PubMed:25024436}.
CC -!- BIOTECHNOLOGY: The encapsulin and a cargo construct (an encB-encC-encD
CC fusion) can be overexpressed in E.coli and in human HEK293T cells. In
CC HEK293T in the presence of 0.5 M ferrous ammonium sulfate
CC nanocompartments can be detected and used as cell markers. Coexpression
CC of this nanocompartment with a larger nanocompartment from
CC Q.thermotolerans (AC A0A0F5HPP7) allows of expression of different
CC sized iron-rich particles. The encapsulin shell proteins are not seen
CC to mix. {ECO:0000269|PubMed:31194509}.
CC -!- SIMILARITY: Belongs to the ferritin-like superfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF92698.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:25024436};
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DR EMBL; CP000113; ABF92698.1; ALT_INIT; Genomic_DNA.
DR PDB; 7S4Q; EM; 3.12 A; F/G/H=105-116.
DR PDBsum; 7S4Q; -.
DR SMR; Q1D3Y8; -.
DR IntAct; Q1D3Y8; 3.
DR MINT; Q1D3Y8; -.
DR STRING; 246197.MXAN_4464; -.
DR EnsemblBacteria; ABF92698; ABF92698; MXAN_4464.
DR KEGG; mxa:MXAN_4464; -.
DR eggNOG; COG3461; Bacteria.
DR HOGENOM; CLU_141525_0_0_7; -.
DR OMA; FWQLIAR; -.
DR Proteomes; UP000002402; Chromosome.
DR GO; GO:0140737; C:encapsulin nanocompartment; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0006744; P:ubiquinone biosynthetic process; IEA:InterPro.
DR InterPro; IPR009078; Ferritin-like_SF.
DR InterPro; IPR011566; Ubq_synth_Coq7.
DR Pfam; PF03232; COQ7; 1.
DR SUPFAM; SSF47240; SSF47240; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Encapsulin nanocompartment; Iron; Iron storage;
KW Metal-binding; Reference proteome.
FT CHAIN 1..130
FT /note="Encapsulin nanocompartment cargo protein EncC"
FT /id="PRO_0000455322"
FT REGION 103..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..130
FT /note="Probable targeting peptide"
FT /evidence="ECO:0000305|PubMed:25024436"
FT MOTIF 61..64
FT /note="Di-iron-binding motif"
FT /evidence="ECO:0000305|PubMed:25024436"
FT BINDING 31
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 31
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 61
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 61
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 64
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT BINDING 64
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q2RVS1"
SQ SEQUENCE 130 AA; 14389 MW; 1AB4FB5D9D535B54 CRC64;
MPQTNPFHSL VPRKMTDTEL ARSIRLNIEA ELDAINLYAA HIDATDNEDA KAILQHVMDE
EREHAALFWE LIARLDPEQA AHAKEAVEKY RLITSGASHE AVEAVGKEGA APSPADVTPE
KRLTVGSLRR
