ID   ENCD_MYXXD              Reviewed;         109 AA.
AC   Q1D9P3;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Encapsulin nanocompartment cargo protein EncD {ECO:0000303|PubMed:25024436};
GN   Name=encD {ECO:0000303|PubMed:25024436}; ORFNames=MXAN_2410;
OS   Myxococcus xanthus (strain DK1622).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=246197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DK1622;
RX   PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA   Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA   Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA   Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA   Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT   "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, AND
RP   DOMAIN.
RC   STRAIN=DK1622;
RX   PubMed=25024436; DOI=10.15252/embj.201488566;
RA   McHugh C.A., Fontana J., Nemecek D., Cheng N., Aksyuk A.A., Heymann J.B.,
RA   Winkler D.C., Lam A.S., Wall J.S., Steven A.C., Hoiczyk E.;
RT   "A virus capsid-like nanocompartment that stores iron and protects bacteria
RT   from oxidative stress.";
RL   EMBO J. 33:1896-1911(2014).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC   STRAIN=DK1622;
RX   PubMed=31194509; DOI=10.1021/acsnano.9b03140;
RA   Sigmund F., Pettinger S., Kube M., Schneider F., Schifferer M.,
RA   Schneider S., Efremova M.V., Pujol-Marti J., Aichler M., Walch A.,
RA   Misgeld T., Dietz H., Westmeyer G.G.;
RT   "Iron-Sequestering Nanocompartments as Multiplexed Electron Microscopy Gene
RT   Reporters.";
RL   ACS Nano 13:8114-8123(2019).
CC   -!- FUNCTION: Cargo protein of a type 1 encapsulin nanocompartment. May
CC       help nucleate Fe atoms in the interior of the encapsulin
CC       nanocompartment (Probable). Present in about 47 copies/encapsulin
CC       nanocompartment (PubMed:25024436). {ECO:0000269|PubMed:25024436,
CC       ECO:0000305|PubMed:25024436}.
CC   -!- SUBCELLULAR LOCATION: Encapsulin nanocompartment
CC       {ECO:0000269|PubMed:25024436}. Note=Probably lies against the interior
CC       face of the nanocompartment. {ECO:0000305|PubMed:25024436}.
CC   -!- BIOTECHNOLOGY: The encapsulin and a cargo construct (an encB-encC-encD
CC       fusion) can be overexpressed in E.coli and in human HEK293T cells. In
CC       HEK293T in the presence of 0.5 M ferrous ammonium sulfate
CC       nanocompartments can be detected and used as cell markers. Coexpression
CC       of this nanocompartment with a larger nanocompartment from
CC       Q.thermotolerans (AC A0A0F5HPP7) allows of expression of different
CC       sized iron-rich particles. The encapsulin shell proteins are not seen
CC       to mix. {ECO:0000269|PubMed:31194509}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000113; ABF90650.1; -; Genomic_DNA.
DR   RefSeq; WP_011552485.1; NC_008095.1.
DR   IntAct; Q1D9P3; 1.
DR   MINT; Q1D9P3; -.
DR   STRING; 246197.MXAN_2410; -.
DR   EnsemblBacteria; ABF90650; ABF90650; MXAN_2410.
DR   GeneID; 41359794; -.
DR   KEGG; mxa:MXAN_2410; -.
DR   HOGENOM; CLU_2262120_0_0_7; -.
DR   OMA; ARWQRIQ; -.
DR   Proteomes; UP000002402; Chromosome.
DR   GO; GO:0140737; C:encapsulin nanocompartment; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Encapsulin nanocompartment; Iron; Iron storage; Metal-binding;
KW   Reference proteome.
FT   CHAIN           1..109
FT                   /note="Encapsulin nanocompartment cargo protein EncD"
FT                   /id="PRO_0000455332"
FT   REGION          61..94
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          100..106
FT                   /note="Probable targeting peptide"
FT                   /evidence="ECO:0000305|PubMed:25024436"
FT   BINDING         47
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
FT   BINDING         47
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q2RVS1"
SQ   SEQUENCE   109 AA;  11509 MW;  63BBDB114E092C28 CRC64;
     MAKNSNPSAF DRDFGYLMPF LDRVAAAASD LEDASARAEL TRLMVEEKAR WQRIQELLGG
     AGGRGAAAPT PAREAPAEAP RLARGSADEL HEAAPFATGL TVGSLRGSR