ID   ENCP2_MYCPA             Reviewed;         307 AA.
AC   I3NID5; Q9KII7;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2012, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Type 2A encapsulin shell protein {ECO:0000250|UniProtKB:Q55032};
DE   AltName: Full=Major membrane protein-1 {ECO:0000303|PubMed:11549181};
DE            Short=MMP-1 {ECO:0000303|PubMed:11549181};
GN   Name=enc2 {ECO:0000305}; OrderedLocusNames=MAP_2121c;
OS   Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
OS   (Mycobacterium paratuberculosis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=262316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-968 / K-10;
RX   PubMed=16116077; DOI=10.1073/pnas.0505662102;
RA   Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N.,
RA   Kanjilal S., Kapur V.;
RT   "The complete genome sequence of Mycobacterium avium subspecies
RT   paratuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-307, AND ANTIGENICITY.
RC   STRAIN=ATCC 19698 / CIP 103963 / DSM 44133 / TMC 807;
RX   PubMed=11549181; DOI=10.1099/0022-1317-50-9-795;
RA   Bannantine J.P., Stabel J.R.;
RT   "Identification of two Mycobacterium avium subspecies paratuberculosis gene
RT   products differentially recognised by sera from rabbits immunised with live
RT   mycobacteria but not heat-killed mycobacteria.";
RL   J. Med. Microbiol. 50:795-804(2001).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CYD, SUBUNIT,
RP   SUBCELLULAR LOCATION, AND ANTIGENICITY.
RC   STRAIN=509;
RX   PubMed=25500374; DOI=10.1016/j.vetmic.2014.11.009;
RA   Leite F.L., Reinhardt T.A., Bannantine J.P., Stabel J.R.;
RT   "Envelope protein complexes of Mycobacterium avium subsp. paratuberculosis
RT   and their antigenicity.";
RL   Vet. Microbiol. 175:275-285(2015).
RN   [4]
RP   CLASSIFICATION.
RX   PubMed=34362927; DOI=10.1038/s41467-021-25071-y;
RA   Andreas M.P., Giessen T.W.;
RT   "Large-scale computational discovery and analysis of virus-derived
RT   microbial nanocompartments.";
RL   Nat. Commun. 12:4748-4748(2021).
CC   -!- FUNCTION: Shell component of a type 2A encapsulin nanocompartment.
CC       Forms encapsulin nanocompartments about 24 nm in diameter from 60
CC       monomers, probably involved in sulfur metabolism (By similarity).
CC       Probably encapsulates cysteine desulfurase (Probable).
CC       {ECO:0000250|UniProtKB:Q55032, ECO:0000305|PubMed:25500374}.
CC   -!- SUBUNIT: The encapsulin nanocompartment is formed by 60 subunits;
CC       monomers form pentamers which assemble to form shells. There are 12
CC       charged pores where the pentamers meet as well as 3-fold axis channels
CC       and dimer channels (By similarity). Isolated from bacteria in a complex
CC       with cysteine desulfurase (AC Q9KII6) (PubMed:25500374).
CC       {ECO:0000250|UniProtKB:Q55032, ECO:0000269|PubMed:25500374}.
CC   -!- SUBCELLULAR LOCATION: Encapsulin nanocompartment
CC       {ECO:0000250|UniProtKB:Q55032}. Cell membrane
CC       {ECO:0000305|PubMed:25500374}.
CC   -!- DOMAIN: Has 4 domains; an N-terminal arm not found in the type 1
CC       subfamily, a discontinuous peripheral domain (P), an elongated loop (E)
CC       and the discontinuous axial domain (A). {ECO:0000250|UniProtKB:Q55032}.
CC   -!- MISCELLANEOUS: Detected in antisera from mice immunized with killed
CC       bacteria but not live bacteria (PubMed:11549181). Cattle naturally
CC       infected with M.paratuberculosis all have antisera that recognize this
CC       protein (PubMed:25500374). {ECO:0000269|PubMed:11549181,
CC       ECO:0000269|PubMed:25500374}.
CC   -!- SIMILARITY: Belongs to the encapsulin family. Family 2A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE016958; AAS04438.1; -; Genomic_DNA.
DR   EMBL; AF232751; AAF82073.1; -; Genomic_DNA.
DR   RefSeq; WP_003872495.1; NC_002944.2.
DR   SMR; I3NID5; -.
DR   STRING; 262316.MAP_2121c; -.
DR   EnsemblBacteria; AAS04438; AAS04438; MAP_2121c.
DR   KEGG; mpa:MAP_2121c; -.
DR   eggNOG; COG0664; Bacteria.
DR   HOGENOM; CLU_089302_0_0_11; -.
DR   OMA; WLVHLLQ; -.
DR   Proteomes; UP000000580; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR045641; MmpI-like_C.
DR   Pfam; PF19307; PCLP; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Encapsulin nanocompartment; Membrane; Reference proteome.
FT   CHAIN           1..307
FT                   /note="Type 2A encapsulin shell protein"
FT                   /id="PRO_0000455320"
SQ   SEQUENCE   307 AA;  33671 MW;  1C4A77899035DAFB CRC64;
     MTSAQNESQA LGDLAARQLA NATKTVPQLS TITPRWLLHL LNWVPVEAGI YRVNRVVNPE
     QVAIKAEAGA GSEEPLPQTY VDYETSPREY TLRSISTLVD IHTRVSDLYS SPHDQIAQQL
     RLTIETIKER QELELINSPE YGLLAQATPE QTIQTLAGAP TPDDLDALIT KVWKTPSFFL
     THPLGIAAFG REATYRGVPP PVVSLFGAQF ITWRGIPLIP SDKVPVEDGK TKFILVRTGE
     ERQGVVGLFQ PGLVGEQAPG LSVRFTGINQ SAIATYLVTL YTSLAVLTDD ALAVLDDVAV
     DQFHEYK