AGD13_ARATH
ID AGD13_ARATH Reviewed; 336 AA.
AC Q8LFN9; Q9M0W2;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable ADP-ribosylation factor GTPase-activating protein AGD13;
DE Short=ARF GAP AGD13;
DE AltName: Full=Protein ARF-GAP DOMAIN 13;
DE Short=AtAGD13;
GN Name=AGD13; OrderedLocusNames=At4g05330; ORFNames=C6L9.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
CC -!- FUNCTION: GTPase-activating protein (GAP) for ADP ribosylation factor
CC (ARF). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB81075.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL161503; CAB81075.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82506.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66129.1; -; Genomic_DNA.
DR EMBL; AY084733; AAM61306.1; -; mRNA.
DR EMBL; BT024733; ABD59071.1; -; mRNA.
DR PIR; A85067; A85067.
DR RefSeq; NP_001328044.1; NM_001340549.1.
DR RefSeq; NP_567292.1; NM_116772.4.
DR AlphaFoldDB; Q8LFN9; -.
DR SMR; Q8LFN9; -.
DR STRING; 3702.AT4G05330.1; -.
DR iPTMnet; Q8LFN9; -.
DR PaxDb; Q8LFN9; -.
DR PRIDE; Q8LFN9; -.
DR ProteomicsDB; 244876; -.
DR EnsemblPlants; AT4G05330.1; AT4G05330.1; AT4G05330.
DR EnsemblPlants; AT4G05330.3; AT4G05330.3; AT4G05330.
DR GeneID; 825881; -.
DR Gramene; AT4G05330.1; AT4G05330.1; AT4G05330.
DR Gramene; AT4G05330.3; AT4G05330.3; AT4G05330.
DR KEGG; ath:AT4G05330; -.
DR Araport; AT4G05330; -.
DR TAIR; locus:2115904; AT4G05330.
DR eggNOG; KOG0703; Eukaryota.
DR eggNOG; KOG1030; Eukaryota.
DR HOGENOM; CLU_045472_0_1_1; -.
DR InParanoid; Q8LFN9; -.
DR OMA; DENIGML; -.
DR OrthoDB; 987311at2759; -.
DR PhylomeDB; Q8LFN9; -.
DR PRO; PR:Q8LFN9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LFN9; baseline and differential.
DR Genevisible; Q8LFN9; AT.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005543; F:phospholipid binding; IEA:InterPro.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR Gene3D; 1.10.220.150; -; 1.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR044518; ARF_GAP_AGD11/12/13.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR PANTHER; PTHR46220; PTHR46220; 1.
DR Pfam; PF01412; ArfGap; 1.
DR Pfam; PF00168; C2; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00105; ArfGap; 1.
DR SMART; SM00239; C2; 1.
DR SUPFAM; SSF49562; SSF49562; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50115; ARFGAP; 1.
DR PROSITE; PS50004; C2; 1.
PE 2: Evidence at transcript level;
KW Calcium; GTPase activation; Metal-binding; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..336
FT /note="Probable ADP-ribosylation factor GTPase-activating
FT protein AGD13"
FT /id="PRO_0000352504"
FT DOMAIN 15..137
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT DOMAIN 162..280
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT ZN_FING 30..53
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT BINDING 249
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 252
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 255
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
SQ SEQUENCE 336 AA; 37279 MW; 574AA30EC478D20B CRC64;
MSNYAAGLGK PGSGKRRIRD LLNQPDNRVC ADCGASDPKW ASANIGVFIC LKCCGVHRSL
GTHISKVLSV TLDEWSDEEV DSMIEIGGNA SANSIYEAFL PDTCSKPGPD VNHDQRMRFI
RAKYELQEFL KPSLRITSGK GSTKSSAFLT SSLSRKIMDS FRTNSSSQTM FQEGMVEFIG
LLKVTIKKGT NLAIRDMMSS DPYVVLNLGK QKLQTTVMNS NLNPVWNQEL MLSVPESYGP
VKLQVYDYDT FSADDIMGEA DIDIQPLITS AMAFGDPEMF GDMQIGKWLK SHDNPLIDDS
IINIVDGKVK QEVQIKLQNV ESGELELEME WLPLDQ
