AGD14_ARATH
ID AGD14_ARATH Reviewed; 649 AA.
AC Q8RXE7; Q67YQ3; Q9FRR6; Q9FRR7; Q9FVH3;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Probable ADP-ribosylation factor GTPase-activating protein AGD14;
DE Short=ARF GAP AGD14;
DE AltName: Full=Protein ARF-GAP DOMAIN 14;
DE Short=AtAGD14;
DE AltName: Full=Protein ZIGA4;
GN Name=AGD14; Synonyms=ZIG4, ZIGA4; OrderedLocusNames=At1g08680/At1g08690;
GN ORFNames=F22O13.16/F22O13.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11202441; DOI=10.1023/a:1026509002161;
RA Jensen R.B., Lykke-Andersen K., Frandsen G.I., Nielsen H.B., Haseloff J.,
RA Jespersen H.M., Mundy J., Skriver K.;
RT "Promiscuous and specific phospholipid binding by domains in ZAC, a
RT membrane-associated Arabidopsis protein with an ARF GAP zinc finger and a
RT C2 domain.";
RL Plant Mol. Biol. 44:799-814(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: GTPase-activating protein (GAP) for ADP ribosylation factor
CC (ARF). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8RXE7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RXE7-2; Sequence=VSP_035554;
CC Name=3;
CC IsoId=Q8RXE7-3; Sequence=VSP_035553;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF99759.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At1g08680 and At1g08690.; Evidence={ECO:0000305};
CC Sequence=AAF99760.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At1g08680 and At1g08690.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF184145; AAG17005.1; -; mRNA.
DR EMBL; AC003981; AAF99759.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC003981; AAF99760.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28331.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28332.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28333.1; -; Genomic_DNA.
DR EMBL; AY081303; AAL91192.1; -; mRNA.
DR EMBL; BT008398; AAP37757.1; -; mRNA.
DR EMBL; AK176415; BAD44178.1; -; mRNA.
DR PIR; T00722; T00722.
DR PIR; T00723; T00723.
DR RefSeq; NP_001031002.1; NM_001035925.2. [Q8RXE7-3]
DR RefSeq; NP_172344.2; NM_100741.4. [Q8RXE7-2]
DR RefSeq; NP_850941.1; NM_180610.4. [Q8RXE7-1]
DR AlphaFoldDB; Q8RXE7; -.
DR SMR; Q8RXE7; -.
DR STRING; 3702.AT1G08680.4; -.
DR iPTMnet; Q8RXE7; -.
DR PaxDb; Q8RXE7; -.
DR EnsemblPlants; AT1G08680.1; AT1G08680.1; AT1G08680. [Q8RXE7-1]
DR EnsemblPlants; AT1G08680.2; AT1G08680.2; AT1G08680. [Q8RXE7-2]
DR EnsemblPlants; AT1G08680.3; AT1G08680.3; AT1G08680. [Q8RXE7-3]
DR GeneID; 837390; -.
DR Gramene; AT1G08680.1; AT1G08680.1; AT1G08680. [Q8RXE7-1]
DR Gramene; AT1G08680.2; AT1G08680.2; AT1G08680. [Q8RXE7-2]
DR Gramene; AT1G08680.3; AT1G08680.3; AT1G08680. [Q8RXE7-3]
DR KEGG; ath:AT1G08680; -.
DR Araport; AT1G08680; -.
DR eggNOG; KOG0702; Eukaryota.
DR HOGENOM; CLU_009449_1_0_1; -.
DR InParanoid; Q8RXE7; -.
DR OMA; PRDMQSH; -.
DR PhylomeDB; Q8RXE7; -.
DR PRO; PR:Q8RXE7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RXE7; baseline and differential.
DR Genevisible; Q8RXE7; AT.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.220.150; -; 1.
DR InterPro; IPR044820; AGD14-like.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR PANTHER; PTHR46085; PTHR46085; 1.
DR Pfam; PF01412; ArfGap; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00105; ArfGap; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50115; ARFGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Metal-binding; Reference proteome;
KW Zinc; Zinc-finger.
FT CHAIN 1..649
FT /note="Probable ADP-ribosylation factor GTPase-activating
FT protein AGD14"
FT /id="PRO_0000352505"
FT DOMAIN 12..130
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT ZN_FING 27..50
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 124..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 366..391
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..145
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..388
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 185..226
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_035553"
FT VAR_SEQ 469
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_035554"
SQ SEQUENCE 649 AA; 71344 MW; F8FD41CFDC5FE8F6 CRC64;
MMGSKREEER NEKIIRGLMK LPPNRRCINC NSLGPQYVCT TFWTFVCMAC SGIHREFTHR
VKSVSMSKFT SKEVEVLQNG GNQRAREIYL KNWDHQRQRL PENSNAERVR EFIKNVYVQK
KYAGANDADK PSKDSQDHVS SEDMTRRANS YHSYSQSPPY DYQYEERRYG KIPLGFTGKS
ASVKGLHAKA SSFVYSPGRF SDHMFEDQFS NEDSAPRASD YSVSSAGDPF RSDIQSPNFQ
QEAEFRSPQF QHSNAPPSEN LFPGRQHQRT TSSGSVRSVD SNFMSIKSYT SGGLGEAVSE
SRQNTGSQQG KTSNHVPLVA ESTKAPIDLF QLPGAPVAQS VDTFQPSIAP RSPPVNLQQA
PQTYSFTPAN SFAGNLGQQP TSRPSELSAP KNEGWASFDN PMPAAKSTNV ITSPGDFQLE
LKIEEILQPS TSMQLPPYPS TVDQHALSIP SPWQEDLSNV LKDVVDNPQP WNAFPDSIEA
NPLDSSRNIH QQVDGASTSS YNTDHQHLES QVLEELSNDG TQTTRIPAGS SAFGFPGNIG
MAPSYSEEAW QHVNEQKSAN PFDLPYDSEF DSNDMFLDMS SLQGALPDIQ TPQAFLNGVS
QPWLAADSVP SYLPAPAVAQ GGLAYMAGQA STNSAAQGPV AFTGGNPFA
