AGD1_ARATH
ID AGD1_ARATH Reviewed; 828 AA.
AC Q9FIT8;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=ADP-ribosylation factor GTPase-activating protein AGD1;
DE Short=ARF GAP AGD1;
DE AltName: Full=Protein ARF-GAP DOMAIN 1;
DE Short=AtAGD1;
GN Name=AGD1; OrderedLocusNames=At5g61980; ORFNames=K22G18.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=18539780; DOI=10.1104/pp.108.119529;
RA Yoo C.-M., Wen J., Motes C.M., Sparks J.A., Blancaflor E.B.;
RT "A class one ADP-ribosylation factor GTPase-activating protein is critical
RT for maintaining directional root hair growth in Arabidopsis thaliana.";
RL Plant Physiol. 147:1659-1674(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=16698946; DOI=10.1105/tpc.105.039008;
RA Sieburth L.E., Muday G.K., King E.J., Benton G., Kim S., Metcalf K.E.,
RA Meyers L., Seamen E., Van Norman J.M.;
RT "SCARFACE encodes an ARF-GAP that is required for normal auxin efflux and
RT vein patterning in Arabidopsis.";
RL Plant Cell 18:1396-1411(2006).
CC -!- FUNCTION: Probable GTPase-activating protein (By similarity). Regulator
CC of membrane trafficking. Required for maintaining a straight growth of
CC root hairs. {ECO:0000250, ECO:0000269|PubMed:18539780}.
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000269|PubMed:18539780}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, but not in hypocotyls or
CC cotyledons. Low levels detected in leaf and shoot apical meristems and
CC in siliques. {ECO:0000269|PubMed:16698946}.
CC -!- DISRUPTION PHENOTYPE: Wavy root hair phenotype.
CC {ECO:0000269|PubMed:18539780}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10160.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB016880; BAB10160.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB022212; BAB10160.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED97545.1; -; Genomic_DNA.
DR RefSeq; NP_201004.2; NM_125591.3.
DR AlphaFoldDB; Q9FIT8; -.
DR SMR; Q9FIT8; -.
DR STRING; 3702.AT5G61980.1; -.
DR PaxDb; Q9FIT8; -.
DR PRIDE; Q9FIT8; -.
DR ProteomicsDB; 244906; -.
DR EnsemblPlants; AT5G61980.1; AT5G61980.1; AT5G61980.
DR GeneID; 836319; -.
DR Gramene; AT5G61980.1; AT5G61980.1; AT5G61980.
DR KEGG; ath:AT5G61980; -.
DR Araport; AT5G61980; -.
DR TAIR; locus:2156176; AT5G61980.
DR eggNOG; KOG0521; Eukaryota.
DR HOGENOM; CLU_016029_1_0_1; -.
DR OMA; SHYHGII; -.
DR OrthoDB; 751525at2759; -.
DR PhylomeDB; Q9FIT8; -.
DR PRO; PR:Q9FIT8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FIT8; baseline and differential.
DR Genevisible; Q9FIT8; AT.
DR GO; GO:0030139; C:endocytic vesicle; IDA:TAIR.
DR GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0035618; C:root hair; IDA:TAIR.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007010; P:cytoskeleton organization; IMP:TAIR.
DR GO; GO:0048768; P:root hair cell tip growth; IMP:TAIR.
DR CDD; cd07606; BAR_SFC_plant; 1.
DR Gene3D; 1.10.220.150; -; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR045258; ACAP1/2/3-like.
DR InterPro; IPR035670; AGD1/2/3/4_BAR_plant.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR InterPro; IPR004148; BAR_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR23180; PTHR23180; 1.
DR Pfam; PF13637; Ank_4; 1.
DR Pfam; PF01412; ArfGap; 1.
DR Pfam; PF00169; PH; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00105; ArfGap; 1.
DR SMART; SM00721; BAR; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF103657; SSF103657; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
DR PROSITE; PS50115; ARFGAP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW ANK repeat; Coiled coil; Endosome; GTPase activation; Metal-binding;
KW Phosphoprotein; Reference proteome; Repeat; Transport; Zinc; Zinc-finger.
FT CHAIN 1..828
FT /note="ADP-ribosylation factor GTPase-activating protein
FT AGD1"
FT /id="PRO_0000352495"
FT DOMAIN 1..225
FT /note="BAR"
FT DOMAIN 288..425
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 498..643
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REPEAT 735..764
FT /note="ANK 1"
FT REPEAT 768..797
FT /note="ANK 2"
FT ZN_FING 513..536
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 247..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 590..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 225..255
FT /evidence="ECO:0000255"
FT COMPBIAS 251..268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 441
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5W7F2"
SQ SEQUENCE 828 AA; 93605 MW; E00BEA3512C4C6FF CRC64;
MHFAKLDDSP MFRQQMQSME ESAELLRLRC LRFYKGCRKY TEGLGEGYDA DIGFVNALES
FGGGHNDPVC VAFGGPVMTK FTIALREIGT YKEVLRSQVE HMLSDRLLQF VNGDVHEVKE
ARKRFDKATI TYDQAREKYL SLRKSTRLDV AATIEEDLHS ARTTFEQARF HLVSALSNAE
SKKRFEFLEA VSGTMDAHLR FFKQGYELLH QMEPFINQVL AYAHQSRECA NYEMASLNER
MQEYQRQVDR ETRNSCVSPT GDGMRHNSRN SQKVIEAVMQ SAAKGKVQTI RQGYLSKRSS
NLRGDWKRRF FILDSRGMLY YYRKPWNWSS GNGSRSVVHR NMASENSPGL LSRWLSSHYH
GGVHDEKPVA RHTVNLLTST IKVDADQTDL RFCFRIISPT KVYTLQAENA QDQMDWIEKI
TGVIASLLSF QTPERAIMRL STVDGGDTFS ASDSGSLADP YDIEQAESGE STVEHPMTGG
NRSRFSGCLQ QHDMVKTEKP IDVLTRVLGN ERCADCGAPE PDWASLNLGV LICIECSGIH
RNLGVHISKV RSLTLDVKVW EPSVLTLFQS LGNVYVNSVW EELLNSESRT SSASRSSGTP
KSDRPRKLLV RKPGFNDPIS VKELFIHAKY SERIFVRKAI DSQHFQAVFQ EIWENVRAND
KKSVYRHIVC SEADVNALRG QASYTVSLPL SKMMQMEETL EAKFKSIEEE FQENPAGYSN
SRGDGESMVR EETSNDCSLL HLACLSADIG MVELLLQYGA KINATDSKGR TPLHHCIISR
RYAIARLLLM RGGDPNAVDK DSNIPVKYAS QTDLNDSELI ALLTDSKR
