AGD2_ARATH
ID AGD2_ARATH Reviewed; 776 AA.
AC Q9C6C3; Q8GWM9; Q9C964;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=ADP-ribosylation factor GTPase-activating protein AGD2;
DE Short=ARF GAP AGD2;
DE AltName: Full=Protein ARF-GAP DOMAIN 2;
DE Short=AtAGD2;
GN Name=AGD2; OrderedLocusNames=At1g60860; ORFNames=F23C21.2, T7P1.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=16698946; DOI=10.1105/tpc.105.039008;
RA Sieburth L.E., Muday G.K., King E.J., Benton G., Kim S., Metcalf K.E.,
RA Meyers L., Seamen E., Van Norman J.M.;
RT "SCARFACE encodes an ARF-GAP that is required for normal auxin efflux and
RT vein patterning in Arabidopsis.";
RL Plant Cell 18:1396-1411(2006).
CC -!- FUNCTION: Probable GTPase-activating protein. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, hypocotyls, cotyledons, leaf
CC and shoot apical meristems and siliques. {ECO:0000269|PubMed:16698946}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC43344.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AC018908; AAG51644.1; -; Genomic_DNA.
DR EMBL; AC079675; AAG51867.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33741.1; -; Genomic_DNA.
DR EMBL; AK118751; BAC43344.1; ALT_SEQ; mRNA.
DR PIR; A96634; A96634.
DR RefSeq; NP_176283.1; NM_104767.4.
DR AlphaFoldDB; Q9C6C3; -.
DR SMR; Q9C6C3; -.
DR STRING; 3702.AT1G60860.1; -.
DR PaxDb; Q9C6C3; -.
DR PRIDE; Q9C6C3; -.
DR ProteomicsDB; 244754; -.
DR EnsemblPlants; AT1G60860.1; AT1G60860.1; AT1G60860.
DR GeneID; 842378; -.
DR Gramene; AT1G60860.1; AT1G60860.1; AT1G60860.
DR KEGG; ath:AT1G60860; -.
DR Araport; AT1G60860; -.
DR TAIR; locus:2025850; AT1G60860.
DR eggNOG; KOG0521; Eukaryota.
DR HOGENOM; CLU_016029_1_0_1; -.
DR InParanoid; Q9C6C3; -.
DR OMA; VDAYHHH; -.
DR OrthoDB; 751525at2759; -.
DR PhylomeDB; Q9C6C3; -.
DR PRO; PR:Q9C6C3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6C3; baseline and differential.
DR Genevisible; Q9C6C3; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07606; BAR_SFC_plant; 1.
DR Gene3D; 1.10.220.150; -; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR045258; ACAP1/2/3-like.
DR InterPro; IPR035670; AGD1/2/3/4_BAR_plant.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR InterPro; IPR004148; BAR_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR23180; PTHR23180; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF01412; ArfGap; 1.
DR Pfam; PF00169; PH; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00105; ArfGap; 1.
DR SMART; SM00721; BAR; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF103657; SSF103657; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
DR PROSITE; PS50115; ARFGAP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW ANK repeat; Coiled coil; GTPase activation; Metal-binding;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..776
FT /note="ADP-ribosylation factor GTPase-activating protein
FT AGD2"
FT /id="PRO_0000352496"
FT DOMAIN 2..226
FT /note="BAR"
FT DOMAIN 290..421
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 467..604
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REPEAT 683..712
FT /note="ANK 1"
FT REPEAT 716..745
FT /note="ANK 2"
FT ZN_FING 482..505
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 248..267
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 776 AA; 87803 MW; 348DEE6D19F1E474 CRC64;
MAGFINLEDS PMFQKQVFSL EGTSDELKDR CQKLYKGVKK FMGALGEAST GVSAFADSLE
EFGAGHDDPV SVSIGGPVIS KFINTLRELS SYKEFLRSQV EHVLLERLTN FMTVDLQEAK
ESRRRFDKAV HSYDQAREKF VSLKKNTRGD IVAELEEDLE NSKSAFEKSR FNLVNSLMTI
EAKKKYEFLE SISAIMDSHF KYFKLGYDLL SQLEPYIHQV LTYAQQSKEQ SKIEQDRFAQ
RIQEFRTQSE LDSQQASAKA DPSDVGGNHV YRAIPRKNVE ANSVSTADKE VTKQGYLLKR
SASLRADWKR RFFVLDNHGS LYYYRNTGNK SAKSQHYYSG LGEHSSGVFG RFRTRHNRSA
SQGSLDCNMI DLRTSLIKLD AEDTDLRLCF RIISPQKTYT LQAENGADRM DWVNKITAAI
TIRLNSHFLQ QSPARYLDKK NTSSGPATEN LTLNQKEDYN QRLNVGDDVL TILREIPGNN
TCAECNAPDP DWASLNLGVL MCIECSGVHR NLGVHISKVR SLTLDVKVWE PTILDLFRNL
GNGYCNSVWE ELLHHLDDDS EKGSTDTLAS VSKPSSEDWF TLKEKYINGK YLEKALVVKD
EREANSTASS RIWEAVQSRN IRDIYRLIVK ADANIINTKF DDITDLDVYH HHHVDAPDEV
KKRHDPNACQ RIKNSNEARN CLQGCSLLHV ACQSGDPILL ELLLQFGADI NMRDYHGRTP
LHHCIASGNN AFAKVLLRRG ARPSIEDGGG LSVLERAMEM GAITDEELFL LLAECQ
