AGD4_ARATH
ID AGD4_ARATH Reviewed; 775 AA.
AC Q9SMX5; O04097;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 2.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=ADP-ribosylation factor GTPase-activating protein AGD4;
DE Short=ARF GAP AGD4;
DE AltName: Full=GCN4-complementing protein 1;
DE AltName: Full=Protein ARF-GAP DOMAIN 4;
DE Short=AtAGD4;
GN Name=AGD4; Synonyms=GCP1; OrderedLocusNames=At1g10870; ORFNames=T19D16.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RA Zimmermann S., Wehrle C., Engelberg D., Frohnmeyer H.;
RT "GCP1, a Zn-finger protein from Arabidopsis thaliana transactivate HIS
RT expression in yeast.";
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=16698946; DOI=10.1105/tpc.105.039008;
RA Sieburth L.E., Muday G.K., King E.J., Benton G., Kim S., Metcalf K.E.,
RA Meyers L., Seamen E., Van Norman J.M.;
RT "SCARFACE encodes an ARF-GAP that is required for normal auxin efflux and
RT vein patterning in Arabidopsis.";
RL Plant Cell 18:1396-1411(2006).
CC -!- FUNCTION: Probable GTPase-activating protein. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, hypocotyls, cotyledons, leaf
CC and shoot apical meristems and siliques. {ECO:0000269|PubMed:16698946}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB65489.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ130878; CAB61505.1; -; mRNA.
DR EMBL; U95973; AAB65489.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28658.1; -; Genomic_DNA.
DR PIR; D86242; D86242.
DR RefSeq; NP_172556.2; NM_100962.4.
DR AlphaFoldDB; Q9SMX5; -.
DR SMR; Q9SMX5; -.
DR STRING; 3702.AT1G10870.1; -.
DR iPTMnet; Q9SMX5; -.
DR PaxDb; Q9SMX5; -.
DR ProteomicsDB; 244729; -.
DR EnsemblPlants; AT1G10870.1; AT1G10870.1; AT1G10870.
DR GeneID; 837629; -.
DR Gramene; AT1G10870.1; AT1G10870.1; AT1G10870.
DR KEGG; ath:AT1G10870; -.
DR Araport; AT1G10870; -.
DR TAIR; locus:2197359; AT1G10870.
DR eggNOG; KOG0521; Eukaryota.
DR HOGENOM; CLU_016029_1_0_1; -.
DR InParanoid; Q9SMX5; -.
DR PhylomeDB; Q9SMX5; -.
DR PRO; PR:Q9SMX5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SMX5; baseline and differential.
DR Genevisible; Q9SMX5; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07606; BAR_SFC_plant; 1.
DR Gene3D; 1.10.220.150; -; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR045258; ACAP1/2/3-like.
DR InterPro; IPR035670; AGD1/2/3/4_BAR_plant.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR InterPro; IPR004148; BAR_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR23180; PTHR23180; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF01412; ArfGap; 1.
DR Pfam; PF00169; PH; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00105; ArfGap; 1.
DR SMART; SM00721; BAR; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF103657; SSF103657; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
DR PROSITE; PS50115; ARFGAP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW ANK repeat; Coiled coil; GTPase activation; Metal-binding;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..775
FT /note="ADP-ribosylation factor GTPase-activating protein
FT AGD4"
FT /id="PRO_5000064627"
FT DOMAIN 2..226
FT /note="BAR"
FT DOMAIN 288..421
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 467..603
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REPEAT 682..711
FT /note="ANK 1"
FT REPEAT 715..744
FT /note="ANK 2"
FT ZN_FING 482..505
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
SQ SEQUENCE 775 AA; 86978 MW; 58BA99FB8BF60115 CRC64;
MATFINLEDS PMFQKQVCSL EGTADELKDR CQKLYKGVKK FMGTLGEASK GESAFAACLE
EFGGGPDDPI SLSIGGPVIS KFINALRELA SYKEFLCSQV EHVLLERLMN FISVDLQEAK
ESRHRFDKAA HSYDQSREKF VSLKKNTRGE IVAELEEDLE NSKSTFEKSR FNLVNSLMTI
EAKKKYEFLE SISAIMDAHL RYFKLGYDLL NQLEPFIHQI LTYAQQSKEQ SKIEQDRLAR
RIQEFRTQSE LDSQQLVANA ESSGANGNRV GGNIPYKNTE TSLTADKEVI KQGYLLKRSS
SLRTDWKRKF FVLDSHGSMY YYRTNGNKSM GSHHHYSGSS DHNTGVFGRF RARHNRSGSL
TEGSLGYNTI DLRTSLIKLD AEDMDLRLCF RIISPQKTYT LQAENGADRM DWVNKITKAI
GTLLNSHFLQ QSPVRYLDKD NSSSAPANAV VSGDQIRHND SRQNIGDDVS TILRGLPGNN
ACAECNAPEP DWASLNLGVL LCIQCSGVHR NLGVHISKVR SLSLDVKVWE PTILDLFRNL
GNVYCNSLWE GLLHLDDDCE DGSALSHASV SKPCPEDSFS VKEKYILGKY LEKALVIKDE
SEANLSAASR IWEAVQSRNI REIYRLIVTT GDVNIINTKF DDITDIDAYH HIDAAEKAVK
KRHDPTVCQR IKESNEPRSC LQGCSLLHVA CHIGDSVLLE LLLQFGADLN IRDYHGRTPL
HHCISSGNHK FAKILLRRGA RPSIEDDGGL SVLERAMEMG AITDEELFLL LAECA
