ENDA_SULTO
ID ENDA_SULTO Reviewed; 180 AA.
AC Q975R3; F9VMV1;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=tRNA-splicing endonuclease {ECO:0000255|HAMAP-Rule:MF_01833};
DE EC=4.6.1.16 {ECO:0000255|HAMAP-Rule:MF_01833};
DE AltName: Full=tRNA-intron endonuclease {ECO:0000255|HAMAP-Rule:MF_01833};
GN Name=endA {ECO:0000255|HAMAP-Rule:MF_01833}; OrderedLocusNames=STK_03580;
OS Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
OS (Sulfolobus tokodaii).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfurisphaera.
OX NCBI_TaxID=273063;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=11572479; DOI=10.1093/dnares/8.4.123;
RA Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M.,
RA Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y.,
RA Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T.,
RA Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K.,
RA Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.;
RT "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon,
RT Sulfolobus tokodaii strain7.";
RL DNA Res. 8:123-140(2001).
CC -!- FUNCTION: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at
CC the 5'- and 3'-splice sites to release the intron. The products are an
CC intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and
CC 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged
CC loops of 3 bases are separated by a stem of 4 bp. {ECO:0000255|HAMAP-
CC Rule:MF_01833}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01833};
CC -!- SUBUNIT: Homotetramer; although the tetramer contains four active
CC sites, only two participate in the cleavage. Therefore, it should be
CC considered as a dimer of dimers. {ECO:0000255|HAMAP-Rule:MF_01833}.
CC -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family. Archaeal
CC short subfamily. {ECO:0000255|HAMAP-Rule:MF_01833}.
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DR EMBL; BA000023; BAK54248.1; -; Genomic_DNA.
DR RefSeq; WP_010978320.1; NC_003106.2.
DR PDB; 2CV8; X-ray; 2.80 A; A/B=1-180.
DR PDBsum; 2CV8; -.
DR AlphaFoldDB; Q975R3; -.
DR SMR; Q975R3; -.
DR STRING; 273063.STK_03580; -.
DR EnsemblBacteria; BAK54248; BAK54248; STK_03580.
DR GeneID; 1458281; -.
DR KEGG; sto:STK_03580; -.
DR PATRIC; fig|273063.9.peg.416; -.
DR eggNOG; arCOG01701; Archaea.
DR OMA; KGPGIDH; -.
DR OrthoDB; 98477at2157; -.
DR BRENDA; 3.1.27.B1; 15396.
DR BRENDA; 4.6.1.16; 15396.
DR EvolutionaryTrace; Q975R3; -.
DR Proteomes; UP000001015; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000213; F:tRNA-intron endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01833; EndA_short; 1.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR InterPro; IPR006678; tRNA_intron_Endonuc_N.
DR InterPro; IPR036740; tRNA_intron_Endonuc_N_sf.
DR InterPro; IPR006676; tRNA_splic.
DR InterPro; IPR016442; tRNA_splic_arch_short.
DR PANTHER; PTHR21227; PTHR21227; 1.
DR Pfam; PF01974; tRNA_int_endo; 1.
DR Pfam; PF02778; tRNA_int_endo_N; 1.
DR PIRSF; PIRSF005285; tRNA_splic_archaea; 1.
DR SUPFAM; SSF53032; SSF53032; 1.
DR SUPFAM; SSF55267; SSF55267; 1.
DR TIGRFAMs; TIGR00324; endA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Lyase; Reference proteome; tRNA processing.
FT CHAIN 1..180
FT /note="tRNA-splicing endonuclease"
FT /id="PRO_0000109482"
FT ACT_SITE 117
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01833"
FT ACT_SITE 125
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01833"
FT ACT_SITE 156
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01833"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 9..12
FT /evidence="ECO:0007829|PDB:2CV8"
FT HELIX 15..25
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 28..30
FT /evidence="ECO:0007829|PDB:2CV8"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 46..49
FT /evidence="ECO:0007829|PDB:2CV8"
FT HELIX 50..58
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 63..66
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:2CV8"
FT HELIX 73..83
FT /evidence="ECO:0007829|PDB:2CV8"
FT HELIX 87..99
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:2CV8"
FT HELIX 108..110
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 112..117
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 128..134
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:2CV8"
FT HELIX 141..144
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 147..149
FT /evidence="ECO:0007829|PDB:2CV8"
FT TURN 152..155
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 157..163
FT /evidence="ECO:0007829|PDB:2CV8"
FT TURN 165..167
FT /evidence="ECO:0007829|PDB:2CV8"
FT STRAND 170..178
FT /evidence="ECO:0007829|PDB:2CV8"
SQ SEQUENCE 180 AA; 20507 MW; 40615B98F1C05085 CRC64;
MIGELVKDKI LIKNIEDARL IYKMGYYGKP IGISKPKSAE EINSELILSL IEGVYLVKKG
KLEIVSNGER LDFERLYQIG VTQIPRFRIL YSVYEDLREK GYVVRSGIKY GADFAVYTIG
PGIEHAPYLV IALDENSQIS SNEILGFGRV SHSTRKELIL GIVNLTNGKI RYIMFKWLKM
