ID   ENDON_BPT5              Reviewed;         145 AA.
AC   Q6QGD4; Q66LR4;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Nicking endonuclease {ECO:0000303|PubMed:24198424};
DE            EC=3.1.21.- {ECO:0000305|PubMed:24198424};
GN   ORFNames=ORF142 {ECO:0000312|EMBL:AAX12079.1},
GN   T5.153 {ECO:0000312|EMBL:AAS77192.1}, T5p149 {ECO:0000312|EMBL:AAU05288.1};
OS   Escherichia phage T5 (Enterobacteria phage T5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX   NCBI_TaxID=2695836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT   "Bacteriophage T5 complete genome.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX12079.1};
RX   PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA   Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA   Hu S.;
RT   "Complete genome sequence of bacteriophage T5.";
RL   Virology 332:45-65(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND FUNCTION.
RC   STRAIN=St0 deletion mutant;
RX   PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA   Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA   Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA   Boulanger P.;
RT   "Insights into bacteriophage T5 structure from analysis of its
RT   morphogenesis genes and protein components.";
RL   J. Virol. 88:1162-1174(2014).
CC   -!- FUNCTION: Endonuclease responsible for the single-chain interruptions
CC       (nicks) located at specific positions in the minus strand of the viral
CC       genome. {ECO:0000269|PubMed:24198424}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305|PubMed:15661140}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAU05288.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY543070; AAS77192.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX12079.1; -; Genomic_DNA.
DR   EMBL; AY692264; AAU05288.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_006981.1; NC_005859.1.
DR   SMR; Q6QGD4; -.
DR   GeneID; 2777677; -.
DR   KEGG; vg:2777677; -.
DR   Proteomes; UP000002107; Genome.
DR   Proteomes; UP000002141; Genome.
DR   Proteomes; UP000002503; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IDA:UniProtKB.
PE   2: Evidence at transcript level;
KW   DNA-binding; Endonuclease; Hydrolase; Late protein; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..145
FT                   /note="Nicking endonuclease"
FT                   /id="PRO_0000435551"
FT   REGION          126..145
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   145 AA;  17013 MW;  79C81E635DCFFD05 CRC64;
     MATNTKYKRD AISIMRDGIK SRYSKDGCCA ICGSSEDLEL HHYHTISQLI KKFAKELQLD
     FTDENIVLSN REAFYKKYEH ELVRDVVTLC QHHHQLLHKV YTKEPPLFSA NKQKAWVQKQ
     KDKIQNPQEK TQVKTETKSG FARFL