ID   ENDOU_XENTR             Reviewed;         293 AA.
AC   B1H3D5;
DT   23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Poly(U)-specific endoribonuclease;
DE            EC=4.6.1.- {ECO:0000250|UniProtKB:Q8JFY9};
DE   AltName: Full=Protein endoU;
DE   AltName: Full=Uridylate-specific endoribonuclease;
DE   AltName: Full=XendoU;
GN   Name=endou;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Poly(U)-specific endoribonuclease involved in the processing
CC       of intron-encoded box C/D snoRNAs, such as U16 and U86. Releases
CC       products that have 2',3'-cyclic phosphate termini at the 3'-end.
CC       {ECO:0000250|UniProtKB:Q8JFY9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridylyl-uridylyl-ribonucleotide-RNA = a 3'-end uridylyl-
CC         2',3'-cyclophospho-uridine-RNA + a 5'-end dephospho-ribonucleoside-
CC         RNA; Xref=Rhea:RHEA:67732, Rhea:RHEA-COMP:13936, Rhea:RHEA-
CC         COMP:17334, Rhea:RHEA-COMP:17335, ChEBI:CHEBI:138284,
CC         ChEBI:CHEBI:173079, ChEBI:CHEBI:173080;
CC         Evidence={ECO:0000250|UniProtKB:Q8JFY9};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67733;
CC         Evidence={ECO:0000250|UniProtKB:Q8JFY9};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ENDOU family. {ECO:0000305}.
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DR   EMBL; BC161355; AAI61355.1; -; mRNA.
DR   RefSeq; NP_001120485.1; NM_001127013.1.
DR   AlphaFoldDB; B1H3D5; -.
DR   SMR; B1H3D5; -.
DR   STRING; 8364.ENSXETP00000035990; -.
DR   PaxDb; B1H3D5; -.
DR   GeneID; 100145603; -.
DR   KEGG; xtr:100145603; -.
DR   CTD; 100145603; -.
DR   Xenbase; XB-GENE-5883501; endoul.
DR   eggNOG; KOG2849; Eukaryota.
DR   HOGENOM; CLU_048034_1_0_1; -.
DR   InParanoid; B1H3D5; -.
DR   OMA; NEMWDAD; -.
DR   OrthoDB; 823332at2759; -.
DR   PhylomeDB; B1H3D5; -.
DR   TreeFam; TF319848; -.
DR   Proteomes; UP000008143; Chromosome 3.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000016505; Expressed in neurula embryo and 13 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; ISS:UniProtKB.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR   CDD; cd21159; XendoU; 1.
DR   InterPro; IPR039787; ENDOU.
DR   InterPro; IPR037227; EndoU-like.
DR   InterPro; IPR018998; EndoU_C.
DR   PANTHER; PTHR12439; PTHR12439; 1.
DR   Pfam; PF09412; XendoU; 1.
DR   SUPFAM; SSF142877; SSF142877; 1.
DR   PROSITE; PS51959; ENDOU; 1.
PE   2: Evidence at transcript level;
KW   Endonuclease; Hydrolase; Lyase; Manganese; Metal-binding; Nuclease;
KW   Nucleus; Reference proteome; RNA-binding.
FT   CHAIN           1..293
FT                   /note="Poly(U)-specific endoribonuclease"
FT                   /id="PRO_0000350629"
FT   DOMAIN          9..286
FT                   /note="EndoU"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
FT   ACT_SITE        163
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
FT   ACT_SITE        179
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
FT   ACT_SITE        225
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
SQ   SEQUENCE   293 AA;  33919 MW;  A0C046DB4E003E96 CRC64;
     MESNRTRGLN HELSKLFNEL WDADENRMKA GKDYRISLQG KAGYVPAGSN QARDSASYPL
     FQFVDEEKLR SRKTFATFIS LLDNYEMDTG VAEVVTPEEI AENNTFLDAI LETKVMKIAH
     DYLVRKNQAK PSRNDFKAQL YTIWFQLYSR APGQGPDSCG FEHVFVGESK RGKEIMGLHN
     WVQFYLQEKR KTIDYKGYVG RQNKSRPDHD DQVLNLQFSW KEMVKPIGSS FIGVSPEFEF
     ALYTVVFLTS QEKMTREVVR VEEYEMQIVV NRHGRYIGTA YPVLLSTNNP DLY