ENDSF_BACTN
ID ENDSF_BACTN Reviewed; 508 AA.
AC Q8A2F6;
DT 13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Endo-4-O-sulfatase {ECO:0000303|PubMed:25002587};
DE EC=3.1.6.- {ECO:0000269|PubMed:25002587};
GN OrderedLocusNames=BT_3349 {ECO:0000312|EMBL:AAO78455.1};
OS Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=226186;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC VPI-5482 / E50;
RX PubMed=12663928; DOI=10.1126/science.1080029;
RA Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA Hooper L.V., Gordon J.I.;
RT "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL Science 299:2074-2076(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC VPI-5482 / E50;
RX PubMed=19321416; DOI=10.1073/pnas.0901529106;
RA Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S.,
RA Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L.,
RA Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C.,
RA Hettich R.L., Gordon J.I.;
RT "Characterizing a model human gut microbiota composed of members of its two
RT dominant bacterial phyla.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009).
RN [3]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=25002587; DOI=10.1074/jbc.m114.573303;
RA Ulmer J.E., Vilen E.M., Namburi R.B., Benjdia A., Beneteau J., Malleron A.,
RA Bonnaffe D., Driguez P.A., Descroix K., Lassalle G., Le Narvor C.,
RA Sandstroem C., Spillmann D., Berteau O.;
RT "Characterization of glycosaminoglycan (GAG) sulfatases from the human gut
RT symbiont Bacteroides thetaiotaomicron reveals the first GAG-specific
RT bacterial endosulfatase.";
RL J. Biol. Chem. 289:24289-24303(2014).
CC -!- FUNCTION: Endosulfatase involved in the degradation of the
CC glycosaminoglycans (GAGs) chondroitin sulfate (CS) and dermatan sulfate
CC (DS). Efficiently hydrolyzes sulfate groups from a broad range of
CC substrate size, including disaccharide to high molecular weight CS and
CC DS polymers. Has a strict specificity for the 4-O-sulfate groups of
CC galactosamine (PubMed:25002587). GAG-specific sulfatases play a key
CC role in the persistence of the major human gut symbiont
CC B.thetaiotaomicron in the host gastrointestinal tract
CC (PubMed:25002587). {ECO:0000269|PubMed:25002587,
CC ECO:0000305|PubMed:25002587}.
CC -!- PTM: The conversion to 3-oxoalanine (also known as C-formylglycine,
CC FGly), of a serine or cysteine residue in prokaryotes and of a cysteine
CC residue in eukaryotes, is critical for catalytic activity.
CC {ECO:0000250|UniProtKB:Q9X759}.
CC -!- SIMILARITY: Belongs to the sulfatase family. {ECO:0000305}.
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DR EMBL; AE015928; AAO78455.1; -; Genomic_DNA.
DR RefSeq; NP_812261.1; NC_004663.1.
DR PDB; 6S21; X-ray; 2.80 A; A/B/C=17-508.
DR PDBsum; 6S21; -.
DR AlphaFoldDB; Q8A2F6; -.
DR SMR; Q8A2F6; -.
DR STRING; 226186.BT_3349; -.
DR PaxDb; Q8A2F6; -.
DR PRIDE; Q8A2F6; -.
DR EnsemblBacteria; AAO78455; AAO78455; BT_3349.
DR KEGG; bth:BT_3349; -.
DR PATRIC; fig|226186.12.peg.3417; -.
DR eggNOG; COG3119; Bacteria.
DR HOGENOM; CLU_006332_9_3_10; -.
DR InParanoid; Q8A2F6; -.
DR OMA; DHGETMC; -.
DR Proteomes; UP000001414; Chromosome.
DR GO; GO:0004065; F:arylsulfatase activity; IBA:GO_Central.
DR Gene3D; 3.40.720.10; -; 1.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR000917; Sulfatase_N.
DR Pfam; PF00884; Sulfatase; 1.
DR SUPFAM; SSF53649; SSF53649; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome.
FT CHAIN 1..508
FT /note="Endo-4-O-sulfatase"
FT /id="PRO_0000446228"
FT MOD_RES 84
FT /note="3-oxoalanine (Ser)"
FT /evidence="ECO:0000250|UniProtKB:Q9X759"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 41..44
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 62..68
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 71..75
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 84..93
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 97..100
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 122..128
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 132..138
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 169..171
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 176..182
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 187..189
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 192..194
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 206..219
FT /evidence="ECO:0007829|PDB:6S21"
FT TURN 220..222
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 231..236
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 247..249
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 252..255
FT /evidence="ECO:0007829|PDB:6S21"
FT TURN 256..260
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 263..265
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 277..281
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 282..305
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 308..310
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 311..317
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 331..335
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 338..341
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 345..348
FT /evidence="ECO:0007829|PDB:6S21"
FT TURN 350..352
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 356..358
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 364..366
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 367..374
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 378..380
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 391..395
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 405..414
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 425..444
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 446..448
FT /evidence="ECO:0007829|PDB:6S21"
FT STRAND 451..458
FT /evidence="ECO:0007829|PDB:6S21"
FT TURN 459..461
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 472..474
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 475..492
FT /evidence="ECO:0007829|PDB:6S21"
FT HELIX 495..498
FT /evidence="ECO:0007829|PDB:6S21"
FT TURN 499..505
FT /evidence="ECO:0007829|PDB:6S21"
SQ SEQUENCE 508 AA; 57857 MW; 5609AC6B0E822904 CRC64;
MGGLTLFAAQ GCKAPKQVAE QAEHPNIIYV FPDQYRNQAM GFWNQEGFRD KVNFRGDPVH
TPNIDTFARE SMVLTSAQSN CPLSSPHRGM LLTGMYPNRS GVPLNCNSTR PISSLRDDAE
CIGDVFSKAG YDCAYFGKLH ADFPTPNDPE NPGQYVETQR PVWDAYTPKE QRHGFNYWYS
YGTFDEHKNP HYWDTDGKRH DPKEWSPLHE SGKVVSYLKN EGNVRDTKKP FFIMVGMNPP
HSPYRSLNDC EEQDFNLYKD QPLDSLLIRP NVDLNMKKAE SVRYYFASVT GVDRAFGQIL
EALKQLGLDK NTVVIFASDH GETMCSQRTD DPKNSPYSES MNIPFLVRFP GKIQPRVDDL
LLSAPDIMPT VLGLCGLGDS IPSEVQGRNF APLFFDEKAE IVRPAGALYI QNLDGEKDKD
GLVQSYFPSS RGIKTARYTL ALYIDRKTKQ LKKSLLFDDV NDPYQLNNLP LDENKEVVEQ
LYREMGTMLK EIDDPWYTEK ILSDRIPY
