ENDUB_XENLA
ID ENDUB_XENLA Reviewed; 292 AA.
AC B1WBB4;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Poly(U)-specific endoribonuclease-B;
DE EC=4.6.1.- {ECO:0000250|UniProtKB:Q8JFY9};
DE AltName: Full=Protein endoU-B;
DE AltName: Full=Uridylate-specific endoribonuclease-B;
DE AltName: Full=XendoU-B;
GN Name=endou-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Poly(U)-specific endoribonuclease involved in the processing
CC of intron-encoded box C/D snoRNAs, such as U16 and U86. Releases
CC products that have 2',3'-cyclic phosphate termini at the 3'-end.
CC {ECO:0000250|UniProtKB:Q8JFY9}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridylyl-uridylyl-ribonucleotide-RNA = a 3'-end uridylyl-
CC 2',3'-cyclophospho-uridine-RNA + a 5'-end dephospho-ribonucleoside-
CC RNA; Xref=Rhea:RHEA:67732, Rhea:RHEA-COMP:13936, Rhea:RHEA-
CC COMP:17334, Rhea:RHEA-COMP:17335, ChEBI:CHEBI:138284,
CC ChEBI:CHEBI:173079, ChEBI:CHEBI:173080;
CC Evidence={ECO:0000250|UniProtKB:Q8JFY9};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67733;
CC Evidence={ECO:0000250|UniProtKB:Q8JFY9};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ENDOU family. {ECO:0000305}.
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DR EMBL; BC161693; AAI61693.1; -; mRNA.
DR RefSeq; NP_001128550.1; NM_001135078.1.
DR AlphaFoldDB; B1WBB4; -.
DR SMR; B1WBB4; -.
DR GeneID; 100189581; -.
DR KEGG; xla:100189581; -.
DR CTD; 100189581; -.
DR Xenbase; XB-GENE-6252901; endoul.S.
DR OMA; NEMWDAD; -.
DR OrthoDB; 823332at2759; -.
DR Proteomes; UP000186698; Chromosome 3S.
DR Bgee; 100189581; Expressed in zone of skin and 19 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0004521; F:endoribonuclease activity; ISS:UniProtKB.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR CDD; cd21159; XendoU; 1.
DR InterPro; IPR039787; ENDOU.
DR InterPro; IPR037227; EndoU-like.
DR InterPro; IPR018998; EndoU_C.
DR PANTHER; PTHR12439; PTHR12439; 1.
DR Pfam; PF09412; XendoU; 1.
DR SUPFAM; SSF142877; SSF142877; 1.
DR PROSITE; PS51959; ENDOU; 1.
PE 2: Evidence at transcript level;
KW Endonuclease; Hydrolase; Lyase; Manganese; Metal-binding; Nuclease;
KW Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..292
FT /note="Poly(U)-specific endoribonuclease-B"
FT /id="PRO_0000350628"
FT DOMAIN 8..285
FT /note="EndoU"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
FT ACT_SITE 162
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
FT ACT_SITE 178
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
FT ACT_SITE 224
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01304"
SQ SEQUENCE 292 AA; 33737 MW; D6B0633E0B50B9C5 CRC64;
MEANRGQVNH ELSKLFNELW DADVNRMKAG KDYRISLQGK AGYVPARSNQ AKDSASYPLF
QFVDEEKLKS KKTFATFISL LDNYEMDTGV AEVVTPEEIA ENNNFLDAIL ETKVMKMAHD
YLVRKNQAKP SQNDFKVQLY SIWFQLYSRA PGSRPDSCGF EHVFVGESKR GKEMMGLHNW
VQFYLQEKRK NIDYKGYVAR QNKSRPDEDD QVLNLQFSWK EMVKPVGSSF IGVSPEFEFA
LYTIVFLASQ AKMSREVIRL EEYELQIVVN RHGRYIGTAY PALLSTNNPD LY
