AGE1_YEAST
ID AGE1_YEAST Reviewed; 482 AA.
AC Q04412; D6VTE4;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=ADP-ribosylation factor GTPase-activating protein effector protein 1;
DE Short=ARF GAP effector protein 1;
DE AltName: Full=Suppressor of ARF1 thermosensitive mutant protein 1;
GN Name=AGE1; Synonyms=SAT1; OrderedLocusNames=YDR524C; ORFNames=D9719.28;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=9677411; DOI=10.1074/jbc.273.31.19792;
RA Zhang C.-J., Cavenagh M.M., Kahn R.A.;
RT "A family of Arf effectors defined as suppressors of the loss of Arf
RT function in the yeast Saccharomyces cerevisiae.";
RL J. Biol. Chem. 273:19792-19796(1998).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION.
RX PubMed=12627398; DOI=10.1002/yea.966;
RA Zhang C.-J., Bowzard J.B., Anido A., Kahn R.A.;
RT "Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and
RT distinct functions.";
RL Yeast 20:315-330(2003).
CC -!- FUNCTION: GTPase-activating protein (GAP) for the ADP ribosylation
CC factors ARF1 and ARF2. May be involved in the endocytic pathway.
CC {ECO:0000269|PubMed:12627398, ECO:0000269|PubMed:9677411}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U33057; AAB64964.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12354.1; -; Genomic_DNA.
DR PIR; S69580; S69580.
DR RefSeq; NP_010812.3; NM_001180832.3.
DR AlphaFoldDB; Q04412; -.
DR SMR; Q04412; -.
DR BioGRID; 32574; 85.
DR DIP; DIP-5273N; -.
DR IntAct; Q04412; 2.
DR MINT; Q04412; -.
DR STRING; 4932.YDR524C; -.
DR iPTMnet; Q04412; -.
DR MaxQB; Q04412; -.
DR PaxDb; Q04412; -.
DR PRIDE; Q04412; -.
DR EnsemblFungi; YDR524C_mRNA; YDR524C; YDR524C.
DR GeneID; 852136; -.
DR KEGG; sce:YDR524C; -.
DR SGD; S000002932; AGE1.
DR VEuPathDB; FungiDB:YDR524C; -.
DR eggNOG; KOG0521; Eukaryota.
DR GeneTree; ENSGT00940000169062; -.
DR HOGENOM; CLU_493595_0_0_1; -.
DR InParanoid; Q04412; -.
DR OMA; THSHHIN; -.
DR BioCyc; YEAST:G3O-30040-MON; -.
DR PRO; PR:Q04412; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04412; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0005802; C:trans-Golgi network; IDA:SGD.
DR GO; GO:0005096; F:GTPase activator activity; IDA:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005543; F:phospholipid binding; IDA:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IGI:SGD.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IGI:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.220.150; -; 1.
DR InterPro; IPR045258; ACAP1/2/3-like.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR PANTHER; PTHR23180; PTHR23180; 1.
DR Pfam; PF01412; ArfGap; 1.
DR SMART; SM00105; ArfGap; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50115; ARFGAP; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; GTPase activation; Metal-binding; Protein transport;
KW Reference proteome; Transport; Zinc; Zinc-finger.
FT CHAIN 1..482
FT /note="ADP-ribosylation factor GTPase-activating protein
FT effector protein 1"
FT /id="PRO_0000074222"
FT DOMAIN 170..297
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT ZN_FING 186..210
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 116..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 482 AA; 54494 MW; 3F7682F91EA21EDD CRC64;
MDFYTTDINK NVVPLFSKGT VARTASKAQY PSWCNNALKL TNILLKSLRC KFQTNRCEDD
RGFEVYCVIL KSIALLMAAK ESLILLQIPP SLPSGFPFRS PQLSFTYLST RLSGSQHKST
HSHHINHQTH PIHSSSSNSN SNNRIPTKTD SSKQHTQHFS FANAGASNRD ELLSIVRKID
KSNLKCCDCG STATVEWVSI NLLCILCIKC SGVHRSLGSH ISKIRSLTLD NFTSLELMHL
LQNNVSNSNV NAIYESNLRN FPVKKITANS DDSERSKFII DKYQFKKFVI DSNQGREASL
KSLIKAIHLD SVFMMQRAIA QSKYSLRELT ASEKEQNDLN HSSIFQYSLK HYEIVDGTPI
FFITEFLLCN GIHIDNLPKI TTNWSPKVLE YWETKLEMYG TFQAVNTSRP RSGPHLNMHS
NVDSASSYNK KHDLRVNIPE RSASASKRWS LSSIPKSSQN LMSPTNLLTM HKSLKLAKKD
KK
