AGFG2_HUMAN
ID AGFG2_HUMAN Reviewed; 481 AA.
AC O95081; O75429; Q96AB9; Q96GL4;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2004, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Arf-GAP domain and FG repeat-containing protein 2;
DE AltName: Full=HIV-1 Rev-binding protein-like protein;
DE AltName: Full=Rev/Rex activation domain-binding protein related;
DE Short=RAB-R;
GN Name=AGFG2; Synonyms=HRBL, RABR;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=9303539; DOI=10.1101/gad.11.17.2239;
RA Salcini A.E., Confalonieri S., Doria M., Santolini E., Tassi E.,
RA Minenkova O., Cesareni G., Pelicci P.G., Di Fiore P.P.;
RT "Binding specificity and in vivo targets of the EH domain, a novel protein-
RT protein interaction module.";
RL Genes Dev. 11:2239-2249(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-481.
RX PubMed=9799793; DOI=10.1101/gr.8.10.1060;
RA Gloeckner G., Scherer S., Schattevoy R., Boright A.P., Weber J.,
RA Tsui L.-C., Rosenthal A.;
RT "Large-scale sequencing of two regions in human chromosome 7q22: analysis
RT of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17
RT genes.";
RL Genome Res. 8:1060-1073(1998).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-173, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- SUBUNIT: Interacts with EPS15R. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O95081-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O95081-2; Sequence=VSP_010667, VSP_010668;
CC -!- DOMAIN: Contains FG repeats and 4 N-P-F repeats.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC78803.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF015042; AAD01550.1; -; mRNA.
DR EMBL; BC009393; AAH09393.1; -; mRNA.
DR EMBL; BC017329; AAH17329.1; -; mRNA.
DR EMBL; AF053356; AAC78803.1; ALT_SEQ; Genomic_DNA.
DR CCDS; CCDS5697.1; -. [O95081-1]
DR RefSeq; NP_006067.3; NM_006076.4. [O95081-1]
DR AlphaFoldDB; O95081; -.
DR SMR; O95081; -.
DR BioGRID; 109504; 21.
DR ELM; O95081; -.
DR IntAct; O95081; 6.
DR STRING; 9606.ENSP00000300176; -.
DR GlyGen; O95081; 11 sites, 2 O-linked glycans (11 sites).
DR iPTMnet; O95081; -.
DR PhosphoSitePlus; O95081; -.
DR BioMuta; AGFG2; -.
DR EPD; O95081; -.
DR jPOST; O95081; -.
DR MassIVE; O95081; -.
DR MaxQB; O95081; -.
DR PaxDb; O95081; -.
DR PeptideAtlas; O95081; -.
DR PRIDE; O95081; -.
DR ProteomicsDB; 50647; -. [O95081-1]
DR ProteomicsDB; 50648; -. [O95081-2]
DR Antibodypedia; 16493; 150 antibodies from 27 providers.
DR DNASU; 3268; -.
DR Ensembl; ENST00000300176.9; ENSP00000300176.4; ENSG00000106351.13. [O95081-1]
DR GeneID; 3268; -.
DR KEGG; hsa:3268; -.
DR MANE-Select; ENST00000300176.9; ENSP00000300176.4; NM_006076.5; NP_006067.3.
DR UCSC; uc003uvf.4; human. [O95081-1]
DR CTD; 3268; -.
DR DisGeNET; 3268; -.
DR GeneCards; AGFG2; -.
DR HGNC; HGNC:5177; AGFG2.
DR HPA; ENSG00000106351; Tissue enriched (salivary).
DR MIM; 604019; gene.
DR neXtProt; NX_O95081; -.
DR NIAGADS; ENSG00000106351; -.
DR OpenTargets; ENSG00000106351; -.
DR PharmGKB; PA29451; -.
DR VEuPathDB; HostDB:ENSG00000106351; -.
DR eggNOG; KOG0702; Eukaryota.
DR GeneTree; ENSGT00940000161071; -.
DR HOGENOM; CLU_027801_0_0_1; -.
DR InParanoid; O95081; -.
DR OMA; SDCKRNK; -.
DR OrthoDB; 1226524at2759; -.
DR PhylomeDB; O95081; -.
DR TreeFam; TF325357; -.
DR PathwayCommons; O95081; -.
DR SignaLink; O95081; -.
DR BioGRID-ORCS; 3268; 11 hits in 1077 CRISPR screens.
DR ChiTaRS; AGFG2; human.
DR GeneWiki; HRBL; -.
DR GenomeRNAi; 3268; -.
DR Pharos; O95081; Tbio.
DR PRO; PR:O95081; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; O95081; protein.
DR Bgee; ENSG00000106351; Expressed in parotid gland and 138 other tissues.
DR ExpressionAtlas; O95081; baseline and differential.
DR Genevisible; O95081; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0005096; F:GTPase activator activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.220.150; -; 1.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR Pfam; PF01412; ArfGap; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00105; ArfGap; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50115; ARFGAP; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Metal-binding; Reference proteome;
KW Repeat; Zinc; Zinc-finger.
FT CHAIN 1..481
FT /note="Arf-GAP domain and FG repeat-containing protein 2"
FT /id="PRO_0000204828"
FT DOMAIN 27..153
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT ZN_FING 47..70
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 150..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 431..481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 173
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT VAR_SEQ 145..155
FT /note="YVPPDQVKGPT -> PDTFPRRLCQL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010667"
FT VAR_SEQ 157..481
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010668"
FT VARIANT 365
FT /note="T -> N (in dbSNP:rs34731997)"
FT /id="VAR_050566"
FT CONFLICT 96
FT /note="V -> L (in Ref. 1; AAD01550)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="D -> N (in Ref. 1; AAD01550)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="Q -> H (in Ref. 3; AAC78803)"
FT /evidence="ECO:0000305"
FT CONFLICT 349
FT /note="M -> T (in Ref. 2; AAH17329)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 481 AA; 48963 MW; A89202A0F0924FE2 CRC64;
MVMAAKKGPG PGGGVSGGKA EAEAASEVWC RRVRELGGCS QAGNRHCFEC AQRGVTYVDI
TVGSFVCTTC SGLLRGLNPP HRVKSISMTT FTEPEVVFLQ SRGNEVCRKI WLGLFDARTS
LVPDSRDPQK VKEFLQEKYE KKRWYVPPDQ VKGPTYTKGS ASTPVQGSIP EGKPLRTLLG
DPAPSLSVAA STSSQPVSQS HARTSQARST QPPPHSSVKK ASTDLLADIG GDPFAAPQMA
PAFAAFPAFG GQTPSQGGFA NFDAFSSGPS SSVFGSLPPA GQASFQAQPT PAGSSQGTPF
GATPLAPASQ PNSLADVGSF LGPGVPAAGV PSSLFGMAGQ VPPLQSVTMG GGGGSSTGLA
FGAFTNPFTA PAAQSPLPST NPFQPNGLAP GPGFGMSSAG PGFPQAVPPT GAFASSFPAP
LFPPQTPLVQ QQNGSSFGDL GSAKLGQRPL SQPAGISTNP FMTGPSSSPF ASKPPTTNPF
L
