AGFG2_MOUSE
ID AGFG2_MOUSE Reviewed; 479 AA.
AC Q80WC7; Q8BKS5; Q99J67;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Arf-GAP domain and FG repeat-containing protein 2;
DE AltName: Full=HIV-1 Rev-binding protein-like protein;
DE AltName: Full=Rev/Rex activation domain-binding protein related;
DE Short=RAB-R;
GN Name=Agfg2; Synonyms=Hrbl, Rabr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 247-479 (ISOFORM 1).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP INTERACTION WITH EPS15R.
RX PubMed=9446614; DOI=10.1074/jbc.273.5.3003;
RA Coda L., Salcini A.E., Confalonieri S., Pelicci G., Sorkina T., Sorkin A.,
RA Pelicci P.G., Di Fiore P.P.;
RT "Eps15R is a tyrosine kinase substrate with characteristics of a docking
RT protein possibly involved in coated pits-mediated internalization.";
RL J. Biol. Chem. 273:3003-3012(1998).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Interacts with EPS15R. {ECO:0000269|PubMed:9446614}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80WC7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80WC7-2; Sequence=VSP_010669, VSP_010670;
CC -!- DOMAIN: Contains FG repeats and 4 N-P-F repeats.
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DR EMBL; BC003330; AAH03330.1; -; mRNA.
DR EMBL; BC046788; AAH46788.1; -; mRNA.
DR EMBL; AK050881; BAC34441.1; -; mRNA.
DR CCDS; CCDS19774.1; -. [Q80WC7-1]
DR RefSeq; NP_835456.1; NM_178162.3. [Q80WC7-1]
DR AlphaFoldDB; Q80WC7; -.
DR BioGRID; 231167; 3.
DR STRING; 10090.ENSMUSP00000031736; -.
DR iPTMnet; Q80WC7; -.
DR PhosphoSitePlus; Q80WC7; -.
DR EPD; Q80WC7; -.
DR MaxQB; Q80WC7; -.
DR PaxDb; Q80WC7; -.
DR PRIDE; Q80WC7; -.
DR ProteomicsDB; 281953; -. [Q80WC7-1]
DR ProteomicsDB; 281954; -. [Q80WC7-2]
DR Antibodypedia; 16493; 150 antibodies from 27 providers.
DR DNASU; 231801; -.
DR Ensembl; ENSMUST00000031736; ENSMUSP00000031736; ENSMUSG00000029722. [Q80WC7-1]
DR GeneID; 231801; -.
DR KEGG; mmu:231801; -.
DR UCSC; uc009adl.2; mouse. [Q80WC7-1]
DR UCSC; uc009ado.3; mouse. [Q80WC7-2]
DR CTD; 3268; -.
DR MGI; MGI:2443267; Agfg2.
DR VEuPathDB; HostDB:ENSMUSG00000029722; -.
DR eggNOG; KOG0702; Eukaryota.
DR GeneTree; ENSGT00940000161071; -.
DR HOGENOM; CLU_027801_1_0_1; -.
DR InParanoid; Q80WC7; -.
DR OMA; SDCKRNK; -.
DR PhylomeDB; Q80WC7; -.
DR TreeFam; TF325357; -.
DR BioGRID-ORCS; 231801; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q80WC7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q80WC7; protein.
DR Bgee; ENSMUSG00000029722; Expressed in thymus and 245 other tissues.
DR ExpressionAtlas; Q80WC7; baseline and differential.
DR Genevisible; Q80WC7; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.220.150; -; 1.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR Pfam; PF01412; ArfGap; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00105; ArfGap; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50115; ARFGAP; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Metal-binding; Reference proteome;
KW Repeat; Zinc; Zinc-finger.
FT CHAIN 1..479
FT /note="Arf-GAP domain and FG repeat-containing protein 2"
FT /id="PRO_0000204829"
FT DOMAIN 27..153
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT ZN_FING 47..70
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 150..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..223
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 174
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:O95081"
FT VAR_SEQ 253..289
FT /note="VGQTPAHGGFANFDAFSSSPSSSTFGSLPPSVQAPFQ -> GKYYPGAGTRL
FT RRICITAKTSLVHRGLTFLCGWGQGA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010669"
FT VAR_SEQ 290..479
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010670"
SQ SEQUENCE 479 AA; 48968 MW; BE299F70C28D6457 CRC64;
MVMAAKKGPG PGGGVGGSKA EAEAASEVWC RRVRELGGCS QAGNRHCFEC AQRGVTYVDI
TVGSFVCTTC SGLLRGLNPP HRVKSISMTT FTEPEVLFLQ SRGNEVCRKI WLGLFDARTS
LIPDSRDPQK VKEFLQEKYE KKRWYVPPEQ VKGPSYSKGS VSATPVQGSV PEGKPIRTLL
GDPVPSLSDP ASTSSQPGSQ SQARSSSQAR SSQPPSHSST KKASTDLLAD IGGDPFAAPQ
VVPAFASFPG FGVGQTPAHG GFANFDAFSS SPSSSTFGSL PPSVQAPFQA QPTPAGSGQM
SAFGVAPLAA ASQPNNLADV GGLLGPRMAA GGLPGSVFGM PSQVPALQSA VPGVSGSGGL
PFGAYTNPFA TPAQAQLPST NPFQPNGLAS GPGFGMSSVR PGLLQPVPPS GAFASPFSAP
VFPTQAGLAD QQNGSSFGDL GTSKLGQRPL SQPAGISTNP FMTGSSAFAS KPPTTNPFL
