ID   AGL10_HALVD             Reviewed;         307 AA.
AC   D4GU63;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Low-salt glycan biosynthesis hexosyltransferase Agl10;
DE            EC=2.4.1.-;
DE   AltName: Full=Archaeal glycosylation protein 10;
GN   Name=agl10; OrderedLocusNames=HVO_2049; ORFNames=C498_05536;
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA   Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA   Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT   "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT   strategies for static and dynamic osmo-response.";
RL   PLoS Genet. 10:E1004784-E1004784(2014).
RN   [3]
RP   FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=24194539; DOI=10.1128/mbio.00716-13;
RA   Kaminski L., Guan Z., Yurist-Doutsch S., Eichler J.;
RT   "Two distinct N-glycosylation pathways process the Haloferax volcanii S-
RT   layer glycoprotein upon changes in environmental salinity.";
RL   MBio 4:E00716-E00716(2013).
CC   -!- FUNCTION: Hexosyltransferase involved in N-glycan biosynthetic pathway
CC       that takes place under low-salt conditions (1.75 M instead of 3.4 M).
CC       Participates in the formation of the tetrasaccharide present at 'Asn-
CC       532' of S-layer glycoprotein Csg, consisting of a sulfated hexose, 2
CC       hexoses and rhamnose. Involved in the addition of final rhamnose (sugar
CC       4) of the tetrasaccharide on the dolichol phosphate carrier.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- PATHWAY: Cell surface structure biogenesis; S-layer biogenesis.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- DISRUPTION PHENOTYPE: Impaired formation of the tetrasaccharide present
CC       at 'Asn-532' of S-layer glycoprotein Csg. No effect on 'Asn-47' and
CC       'Asn-117' glycosylation of S-layer glycoprotein Csg.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; CP001956; ADE02788.1; -; Genomic_DNA.
DR   EMBL; AOHU01000038; ELY34565.1; -; Genomic_DNA.
DR   RefSeq; WP_004041935.1; NZ_AOHU01000038.1.
DR   AlphaFoldDB; D4GU63; -.
DR   SMR; D4GU63; -.
DR   STRING; 309800.C498_05536; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; ADE02788; ADE02788; HVO_2049.
DR   EnsemblBacteria; ELY34565; ELY34565; C498_05536.
DR   GeneID; 8926717; -.
DR   KEGG; hvo:HVO_2049; -.
DR   eggNOG; arCOG01383; Archaea.
DR   HOGENOM; CLU_023845_4_1_2; -.
DR   OMA; DICISEF; -.
DR   OrthoDB; 74894at2157; -.
DR   UniPathway; UPA00378; -.
DR   UniPathway; UPA00977; -.
DR   Proteomes; UP000008243; Chromosome.
DR   Proteomes; UP000011532; Unassembled WGS sequence.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR   GO; GO:0045232; P:S-layer organization; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..307
FT                   /note="Low-salt glycan biosynthesis hexosyltransferase
FT                   Agl10"
FT                   /id="PRO_0000428771"
SQ   SEQUENCE   307 AA;  35296 MW;  40C71958F2848B0B CRC64;
     MPYCLYDNTC KVQQTMLDDL SIIIVNYKSE EDIKNLYSRI SGVAEFVVVD NSSSSELRQW
     CSHDDIHYID SGGNHGYSGG NNMGIRYSLD ELNRESVLVL NPDLEINKED IRELYTIHQS
     TNYSIISPRI LNREGIPVNE SPTPEGTLFR SIGLLPALPG KEHNLKPVDH AHGSCMMISE
     SVFEQIGYLN ESFFMYYEEI EFCYRARREN INIGQCQVVD AIHNQPVDQT RFESSYQVYL
     DFRNRFLASN SIFSKSIDRI QYTTLSILIS GWMVVRMLFE KKIEYIAPAI FGALHGIQNR
     TGKPERM