AGL14_ARATH
ID AGL14_ARATH Reviewed; 221 AA.
AC Q38838; Q0WKU9; Q9T056;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Agamous-like MADS-box protein AGL14 {ECO:0000305};
DE AltName: Full=Protein XAANTAL 2 {ECO:0000303|PubMed:24121311};
GN Name=AGL14 {ECO:0000303|PubMed:12837945};
GN Synonyms=XAL2 {ECO:0000303|PubMed:24121311};
GN OrderedLocusNames=At4g11880 {ECO:0000312|Araport:AT4G11880};
GN ORFNames=T26M18.90;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 7-221, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta; TISSUE=Root;
RX PubMed=7549482; DOI=10.2307/3870100;
RA Rounsley S.D., Ditta G.S., Yanofsky M.F.;
RT "Diverse roles for MADS box genes in Arabidopsis development.";
RL Plant Cell 7:1259-1269(1995).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [7]
RP INTERACTION WITH AGL16.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY AUXIN, AND DISRUPTION PHENOTYPE.
RX PubMed=24121311; DOI=10.1038/emboj.2013.216;
RA Garay-Arroyo A., Ortiz-Moreno E., de la Paz Sanchez M., Murphy A.S.,
RA Garcia-Ponce B., Marsch-Martinez N., de Folter S., Corvera-Poire A.,
RA Jaimes-Miranda F., Pacheco-Escobedo M.A., Dubrovsky J.G., Pelaz S.,
RA Alvarez-Buylla E.R.;
RT "The MADS transcription factor XAL2/AGL14 modulates auxin transport during
RT Arabidopsis root development by regulating PIN expression.";
RL EMBO J. 32:2884-2895(2013).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=25636918; DOI=10.1016/j.molp.2015.01.017;
RA Perez-Ruiz R.V., Garcia-Ponce B., Marsch-Martinez N.,
RA Ugartechea-Chirino Y., Villajuana-Bonequi M., de Folter S., Azpeitia E.,
RA Davila-Velderrain J., Cruz-Sanchez D., Garay-Arroyo A.,
RA de la Paz Sanchez M., Estevez-Palmas J.M., Alvarez-Buylla E.R.;
RT "XAANTAL2 (AGL14) is an important component of the complex gene regulatory
RT network that underlies Arabidopsis shoot apical meristem transitions.";
RL Mol. Plant 8:796-813(2015).
CC -!- FUNCTION: Transcriptional activator that regulates root development by
CC controlling meristem size and patterning of the root apical meristem.
CC Regulates auxin transport and gradients in the root meristematic cells
CC via direct regulation of the auxin efflux carrier PIN1 and PIN4 gene
CC expression. Binds specifically to the CArG-box DNA sequences in the
CC promoter regions of PIN1 and PIN4 genes (PubMed:24121311). Involved in
CC the regulation of shoot apical meristem (SAM) cell identities and
CC transitions. Promotes flowering transition and participates in flower
CC meristem maintenance and determinacy. Positively regulates TFL1 and WUS
CC expression. Binds directly to the TFL1 regulatory sequences
CC (PubMed:25636918). {ECO:0000269|PubMed:24121311}.
CC -!- SUBUNIT: Interacts with AGL16. {ECO:0000269|PubMed:15805477}.
CC -!- INTERACTION:
CC Q38838; O82794: AGL24; NbExp=6; IntAct=EBI-622067, EBI-592083;
CC Q38838; Q9FVC1: SVP; NbExp=4; IntAct=EBI-622067, EBI-592058;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in roots (PubMed:7549482).
CC Expressed in lateral root cap, root epidermis, root endodermis,
CC columella of the root meristematic region, the vascular cylinder in
CC differentiated zones of the primary root and in emerged lateral root
CC primordia (PubMed:24121311). Expressed in pollen (PubMed:12949148).
CC {ECO:0000269|PubMed:12949148, ECO:0000269|PubMed:24121311,
CC ECO:0000269|PubMed:7549482}.
CC -!- DEVELOPMENTAL STAGE: During floral transition, expressed very early at
CC the flanks of the inflorescence meristem in the anlagens upon the
CC transition to flowering Subsequently, expression levels increase in the
CC first and second stages of the floral meristem and at stage 3,
CC expression is restricted to the L1 and L2 layers. Later on, expressed
CC in the gynoecium and stamen primordia at stage 6.
CC {ECO:0000269|PubMed:25636918}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:24121311}.
CC -!- DISRUPTION PHENOTYPE: Retarded root growth, and altered root meristem
CC size and stem-cell patterning (PubMed:24121311). Late flowering
CC phenotype (PubMed:25636918). {ECO:0000269|PubMed:24121311,
CC ECO:0000269|PubMed:25636918}.
CC -!- MISCELLANEOUS: XAANTAL is the Mayan word for 'go slower' in recognition
CC of the retarded root growth phenotypes of xaantal mutants.
CC {ECO:0000303|PubMed:24121311}.
CC -!- MISCELLANEOUS: Plants over-expressing AGL14/XAL2 show early flowering
CC phenotype and flowers have vegetative traits.
CC {ECO:0000269|PubMed:25636918}.
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DR EMBL; AL078606; CAB44326.1; -; Genomic_DNA.
DR EMBL; AL161532; CAB78231.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83062.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67706.1; -; Genomic_DNA.
DR EMBL; AK230464; BAF02258.1; -; mRNA.
DR EMBL; U20184; AAC49082.1; -; mRNA.
DR PIR; T09347; T09347.
DR RefSeq; NP_001319907.1; NM_001340739.1.
DR RefSeq; NP_192925.1; NM_117258.5.
DR AlphaFoldDB; Q38838; -.
DR SMR; Q38838; -.
DR BioGRID; 12093; 9.
DR DIP; DIP-33798N; -.
DR IntAct; Q38838; 19.
DR STRING; 3702.AT4G11880.1; -.
DR PaxDb; Q38838; -.
DR PRIDE; Q38838; -.
DR EnsemblPlants; AT4G11880.1; AT4G11880.1; AT4G11880.
DR EnsemblPlants; AT4G11880.4; AT4G11880.4; AT4G11880.
DR GeneID; 826795; -.
DR Gramene; AT4G11880.1; AT4G11880.1; AT4G11880.
DR Gramene; AT4G11880.4; AT4G11880.4; AT4G11880.
DR KEGG; ath:AT4G11880; -.
DR Araport; AT4G11880; -.
DR TAIR; locus:2137070; AT4G11880.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_4_1; -.
DR InParanoid; Q38838; -.
DR OMA; SRCFSIN; -.
DR OrthoDB; 1307109at2759; -.
DR PhylomeDB; Q38838; -.
DR PRO; PR:Q38838; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q38838; baseline and differential.
DR Genevisible; Q38838; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0010076; P:maintenance of floral meristem identity; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:2000012; P:regulation of auxin polar transport; IMP:UniProtKB.
DR GO; GO:0010082; P:regulation of root meristem growth; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:UniProtKB.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Flowering; Growth regulation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..221
FT /note="Agamous-like MADS-box protein AGL14"
FT /id="PRO_0000199473"
FT DOMAIN 3..57
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 87..177
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT CONFLICT 103
FT /note="H -> D (in Ref. 4; AAC49082)"
FT /evidence="ECO:0000305"
FT CONFLICT 187..188
FT /note="ST -> TS (in Ref. 4; AAC49082)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 25492 MW; E876DAEA049B125E CRC64;
MVRGKTEMKR IENATSRQVT FSKRRNGLLK KAFELSVLCD AEVALIIFSP RGKLYEFSSS
SSIPKTVERY QKRIQDLGSN HKRNDNSQQS KDETYGLARK IEHLEISTRK MMGEGLDASS
IEELQQLENQ LDRSLMKIRA KKYQLLREET EKLKEKERNL IAENKMLMEK CEMQGRGIIG
RISSSSSTSE LDIDDNEMEV VTDLFIGPPE TRHFKKFPPS N
