AGL15_ARATH
ID AGL15_ARATH Reviewed; 268 AA.
AC Q38847; Q38839;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Agamous-like MADS-box protein AGL15;
GN Name=AGL15; OrderedLocusNames=At5g13790; ORFNames=MXE10.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Wassilewskija;
RX PubMed=7549483; DOI=10.2307/3870101;
RA Heck G.R., Perry S.E., Nichols K.N., Fernandez D.E.;
RT "AGL15, a MADS domain protein expressed in developing embryos.";
RL Plant Cell 7:1271-1282(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 7-268.
RC STRAIN=cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=7549482; DOI=10.2307/3870100;
RA Rounsley S.D., Ditta G.S., Yanofsky M.F.;
RT "Diverse roles for MADS box genes in Arabidopsis development.";
RL Plant Cell 7:1259-1269(1995).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Wassilewskija;
RX PubMed=8953767; DOI=10.2307/3870406;
RA Perry S.E., Nichols K.W., Fernandez D.E.;
RT "The MADS domain protein AGL15 localizes to the nucleus during early stages
RT of seed development.";
RL Plant Cell 8:1977-1989(1996).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10318690; DOI=10.1104/pp.120.1.121;
RA Perry S.E., Lehti M.D., Fernandez D.E.;
RT "The MADS-domain protein AGAMOUS-like 15 accumulates in embryonic tissues
RT with diverse origins.";
RL Plant Physiol. 120:121-130(1999).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=10662856; DOI=10.2307/3870921;
RA Fernandez D.E., Heck G.R., Perry S.E., Patterson S.E., Bleecker A.B.,
RA Fang S.-C.;
RT "The embryo MADS domain factor AGL15 acts postembryonically. Inhibition of
RT perianth senescence and abscission via constitutive expression.";
RL Plant Cell 12:183-198(2000).
RN [9]
RP FUNCTION.
RX PubMed=12226488; DOI=10.1104/pp.004721;
RA Fang S.-C., Fernandez D.E.;
RT "Effect of regulated overexpression of the MADS domain factor AGL15 on
RT flower senescence and fruit maturation.";
RL Plant Physiol. 130:78-89(2002).
RN [10]
RP FUNCTION.
RX PubMed=12743119; DOI=10.1074/jbc.m212976200;
RA Tang W., Perry S.E.;
RT "Binding site selection for the plant MADS domain protein AGL15: an in
RT vitro and in vivo study.";
RL J. Biol. Chem. 278:28154-28159(2003).
RN [11]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [12]
RP FUNCTION.
RX PubMed=14615187; DOI=10.1016/s1055-7903(03)00207-0;
RA Becker A., Theissen G.;
RT "The major clades of MADS-box genes and their role in the development and
RT evolution of flowering plants.";
RL Mol. Phylogenet. Evol. 29:464-489(2003).
RN [13]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [14]
RP FUNCTION.
RX PubMed=15084721; DOI=10.1105/tpc.021261;
RA Wang H., Caruso L.V., Downie A.B., Perry S.E.;
RT "The embryo MADS domain protein AGAMOUS-Like 15 directly regulates
RT expression of a gene encoding an enzyme involved in gibberellin
RT metabolism.";
RL Plant Cell 16:1206-1219(2004).
RN [15]
RP INTERACTION WITH AGL15; SVP; AGL24; AP1; AGL6; AG; AGL1; AGL11; AGL5;
RP AGL16; SOC1 AND AGL21.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [16]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION BY AUXIN.
RC STRAIN=cv. Wassilewskija;
RX PubMed=15686521; DOI=10.1111/j.1365-313x.2004.02320.x;
RA Zhu C., Perry S.E.;
RT "Control of expression and autoregulation of AGL15, a member of the MADS-
RT box family.";
RL Plant J. 41:583-594(2005).
RN [17]
RP TISSUE SPECIFICITY.
RX PubMed=16028119; DOI=10.1007/s11103-005-4546-3;
RA Lehti-Shiu M.D., Adamczyk B.J., Fernandez D.E.;
RT "Expression of MADS-box genes during the embryonic phase in Arabidopsis.";
RL Plant Mol. Biol. 58:89-107(2005).
RN [18]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=17521410; DOI=10.1111/j.1365-313x.2007.03105.x;
RA Adamczyk B.J., Lehti-Shiu M.D., Fernandez D.E.;
RT "The MADS domain factors AGL15 and AGL18 act redundantly as repressors of
RT the floral transition in Arabidopsis.";
RL Plant J. 50:1007-1019(2007).
RN [19]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=18305206; DOI=10.1104/pp.108.115832;
RA Thakare D., Tang W., Hill K., Perry S.E.;
RT "The MADS-domain transcriptional regulator AGAMOUS-LIKE15 promotes somatic
RT embryo development in Arabidopsis and soybean.";
RL Plant Physiol. 146:1663-1672(2008).
RN [20]
RP FUNCTION AS TRANSCRIPTION FACTOR.
RC STRAIN=cv. Columbia;
RX PubMed=19269998; DOI=10.1093/jxb/ern351;
RA Nakaminami K., Hill K., Perry S.E., Sentoku N., Long J.A., Karlson D.T.;
RT "Arabidopsis cold shock domain proteins: relationships to floral and
RT silique development.";
RL J. Exp. Bot. 60:1047-1062(2009).
RN [21]
RP FUNCTION AS TRANSCRIPTION ACTIVATOR.
RC STRAIN=cv. Columbia;
RX PubMed=19767455; DOI=10.1105/tpc.109.068890;
RA Zheng Y., Ren N., Wang H., Stromberg A.J., Perry S.E.;
RT "Global identification of targets of the Arabidopsis MADS domain protein
RT AGAMOUS-Like15.";
RL Plant Cell 21:2563-2577(2009).
CC -!- FUNCTION: Transcription factor involved in the negative regulation of
CC flowering, probably through the photoperiodic pathway. Acts as both an
CC activator and a repressor of transcription. Binds DNA in a sequence-
CC specific manner in large CArG motif 5'-CC (A/T)8 GG-3'. Participates
CC probably in the regulation of programs active during the early stages
CC of embryo development. Prevents premature perianth senescence and
CC abscission, fruits development and seed desiccation. Stimulates the
CC expression of at least DTA4, LEC2, FUS3, ABI3, AT4G38680/CSP2 and
CC GRP2B/CSP4. Can enhance somatic embryo development in vitro.
CC {ECO:0000269|PubMed:10318690, ECO:0000269|PubMed:10662856,
CC ECO:0000269|PubMed:12226488, ECO:0000269|PubMed:12743119,
CC ECO:0000269|PubMed:14615187, ECO:0000269|PubMed:15084721,
CC ECO:0000269|PubMed:15686521, ECO:0000269|PubMed:17521410,
CC ECO:0000269|PubMed:18305206, ECO:0000269|PubMed:19269998,
CC ECO:0000269|PubMed:19767455, ECO:0000269|PubMed:8953767}.
CC -!- SUBUNIT: Homodimer. Interacts with SVP, AGL24, AP1, AGL6, AG, AGL1,
CC AGL11, AGL5, AGL16, SOC1 and AGL21. {ECO:0000269|PubMed:15805477}.
CC -!- INTERACTION:
CC Q38847; Q9SZJ6: AGL21; NbExp=5; IntAct=EBI-622076, EBI-621986;
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Associated with the
CC chromosomes during mitosis. Accumulates in the egg cytoplasm before
CC fertilization but moves into the nucleus of the fertilized egg.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in flowers and siliques.
CC Also present in seedlings. Detected during embryogenesis and
CC accumulates during early seed development (at protein level). Expressed
CC in shoot apices and the base of leaf petioles.
CC {ECO:0000269|PubMed:10318690, ECO:0000269|PubMed:10662856,
CC ECO:0000269|PubMed:12837945, ECO:0000269|PubMed:12949148,
CC ECO:0000269|PubMed:15686521, ECO:0000269|PubMed:16028119,
CC ECO:0000269|PubMed:17521410, ECO:0000269|PubMed:8953767}.
CC -!- DEVELOPMENTAL STAGE: During the reproductive phase, accumulates in
CC immature buds and at the base of the floral organs, and in the
CC receptacle, ovules, anther filaments, and stigma and style of open
CC flowers. Accumulates before fertilization in the cytoplasm in the cells
CC of the egg apparatus and moves into the nucleus during early stages of
CC development following fertilization in the suspensor, embryo, and
CC endosperm, mainly double fertilization derived tissues (at protein
CC level). Highly expressed in developing embryos. In young seedlings,
CC present in the shoot and root apices, lateral root primordia and
CC throughout the vascular system. {ECO:0000269|PubMed:10318690,
CC ECO:0000269|PubMed:10662856, ECO:0000269|PubMed:15686521,
CC ECO:0000269|PubMed:17521410, ECO:0000269|PubMed:8953767}.
CC -!- INDUCTION: By auxin (2,4-D). Feedback loop leading to direct down-
CC regulation by itself. {ECO:0000269|PubMed:15686521}.
CC -!- DISRUPTION PHENOTYPE: Early flowering under short-days conditions (SD)
CC when combined with AGL18 disruption. Decreased ability to produce
CC somatic embryos in vitro. {ECO:0000269|PubMed:17521410,
CC ECO:0000269|PubMed:18305206}.
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DR EMBL; U22528; AAA65653.1; -; Genomic_DNA.
DR EMBL; AB011484; BAB10600.1; -; Genomic_DNA.
DR EMBL; AB005230; BAB10600.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED91941.1; -; Genomic_DNA.
DR EMBL; U20185; AAC49083.1; -; mRNA.
DR PIR; S71200; S71200.
DR RefSeq; NP_196883.1; NM_121382.3.
DR AlphaFoldDB; Q38847; -.
DR SMR; Q38847; -.
DR BioGRID; 16502; 45.
DR IntAct; Q38847; 23.
DR STRING; 3702.AT5G13790.1; -.
DR PaxDb; Q38847; -.
DR PRIDE; Q38847; -.
DR ProteomicsDB; 244678; -.
DR EnsemblPlants; AT5G13790.1; AT5G13790.1; AT5G13790.
DR GeneID; 831224; -.
DR Gramene; AT5G13790.1; AT5G13790.1; AT5G13790.
DR KEGG; ath:AT5G13790; -.
DR Araport; AT5G13790; -.
DR TAIR; locus:2177125; AT5G13790.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_2_1; -.
DR PhylomeDB; Q38847; -.
DR PRO; PR:Q38847; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q38847; baseline and differential.
DR Genevisible; Q38847; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0071365; P:cellular response to auxin stimulus; IEP:UniProtKB.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IDA:TAIR.
DR GO; GO:0010227; P:floral organ abscission; IMP:TAIR.
DR GO; GO:0060867; P:fruit abscission; IMP:UniProtKB.
DR GO; GO:0010047; P:fruit dehiscence; IMP:UniProtKB.
DR GO; GO:0045487; P:gibberellin catabolic process; TAS:TAIR.
DR GO; GO:0060862; P:negative regulation of floral organ abscission; IMP:UniProtKB.
DR GO; GO:0009910; P:negative regulation of flower development; IMP:UniProtKB.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:TAIR.
DR GO; GO:2000692; P:negative regulation of seed maturation; IMP:UniProtKB.
DR GO; GO:0048577; P:negative regulation of short-day photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010262; P:somatic embryogenesis; IMP:UniProtKB.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Developmental protein; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..268
FT /note="Agamous-like MADS-box protein AGL15"
FT /id="PRO_0000199474"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 80..170
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT REGION 205..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 268 AA; 30329 MW; E1964D083614220D CRC64;
MGRGKIEIKR IENANSRQVT FSKRRSGLLK KARELSVLCD AEVAVIVFSK SGKLFEYSST
GMKQTLSRYG NHQSSSASKA EEDCAEVDIL KDQLSKLQEK HLQLQGKGLN PLTFKELQSL
EQQLYHALIT VRERKERLLT NQLEESRLKE QRAELENETL RRQVQELRSF LPSFTHYVPS
YIKCFAIDPK NALINHDSKC SLQNTDSDTT LQLGLPGEAH DRRTNEGERE SPSSDSVTTN
TSSETAERGD QSSLANSPPE AKRQRFSV
