ID   ENKUR_BOVIN             Reviewed;         259 AA.
AC   E1B836;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 2.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Enkurin;
GN   Name=ENKUR;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913 {ECO:0000312|Proteomes:UP000009136};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hereford;
RX   PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
RA   Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D.,
RA   Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G.,
RA   Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
RT   "A whole-genome assembly of the domestic cow, Bos taurus.";
RL   Genome Biol. 10:R42.01-R42.10(2009).
RN   [2] {ECO:0007744|PDB:7RRO}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), FUNCTION, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA   Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA   Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA   Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT   "De novo identification of mammalian ciliary motility proteins using cryo-
RT   EM.";
RL   Cell 184:5791-5806.e19(2021).
CC   -!- FUNCTION: Adapter that functions to localize a calcium-sensitive signal
CC       transduction machinery in sperm to a calcium-permeable ion channel.
CC       Microtubule inner protein (MIP) part of the dynein-decorated doublet
CC       microtubules (DMTs) in cilia axoneme, which is required for motile
CC       cilia beating (PubMed:34715025). {ECO:0000250|UniProtKB:Q6SP97,
CC       ECO:0000269|PubMed:34715025}.
CC   -!- SUBUNIT: Binds calmodulin via its IQ domain. Interacts with TRPC1,
CC       TRPC2, TRPC5, but not TRPC3 (By similarity). Interacts with CFAP45 (By
CC       similarity). {ECO:0000250|UniProtKB:Q6SP97,
CC       ECO:0000250|UniProtKB:Q8TC29}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC       {ECO:0000250|UniProtKB:Q6SP97}. Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000269|PubMed:34715025}. Note=Sperm acrosomal crescent and
CC       flagellar principal piece. {ECO:0000250|UniProtKB:Q6SP97}.
CC   -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- DOMAIN: The IQ motif is involved in calmodulin binding.
CC       {ECO:0000250|UniProtKB:Q6SP97}.
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DR   RefSeq; NP_001179374.1; NM_001192445.1.
DR   PDB; 7RRO; EM; 3.40 A; h/i/j/k=1-259.
DR   PDBsum; 7RRO; -.
DR   SMR; E1B836; -.
DR   STRING; 9913.ENSBTAP00000020682; -.
DR   PaxDb; E1B836; -.
DR   Ensembl; ENSBTAT00000020682.6; ENSBTAP00000020682.5; ENSBTAG00000015569.6.
DR   GeneID; 516469; -.
DR   KEGG; bta:516469; -.
DR   CTD; 219670; -.
DR   VEuPathDB; HostDB:ENSBTAG00000015569; -.
DR   VGNC; VGNC:28496; ENKUR.
DR   eggNOG; ENOG502QT8E; Eukaryota.
DR   GeneTree; ENSGT00940000153866; -.
DR   HOGENOM; CLU_088051_1_0_1; -.
DR   InParanoid; E1B836; -.
DR   OMA; YISTFRP; -.
DR   OrthoDB; 1449984at2759; -.
DR   TreeFam; TF323892; -.
DR   Proteomes; UP000009136; Chromosome 13.
DR   GO; GO:0097728; C:9+0 motile cilium; IEA:Ensembl.
DR   GO; GO:0001669; C:acrosomal vesicle; IBA:GO_Central.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   GO; GO:0097228; C:sperm principal piece; IEA:Ensembl.
DR   GO; GO:0005516; F:calmodulin binding; IBA:GO_Central.
DR   GO; GO:0061966; P:establishment of left/right asymmetry; IEA:Ensembl.
DR   GO; GO:0030317; P:flagellated sperm motility; IEA:Ensembl.
DR   InterPro; IPR026150; Enkur.
DR   InterPro; IPR027012; Enkurin_dom.
DR   PANTHER; PTHR21490:SF0; PTHR21490:SF0; 1.
DR   Pfam; PF13864; Enkurin; 1.
DR   PROSITE; PS51665; ENKURIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Calmodulin-binding; Cell projection; Cilium; Coiled coil;
KW   Cytoplasm; Cytoskeleton; Flagellum; Reference proteome; SH3-binding.
FT   CHAIN           1..259
FT                   /note="Enkurin"
FT                   /id="PRO_0000456166"
FT   DOMAIN          163..255
FT                   /note="Enkurin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01000"
FT   DOMAIN          179..190
FT                   /note="IQ"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TC29"
FT   REGION          163..258
FT                   /note="Interaction with TRPC proteins"
FT                   /evidence="ECO:0000250|UniProtKB:Q6SP97"
FT   MOTIF           86..92
FT                   /note="SH3-binding"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   259 AA;  30157 MW;  188914A327839AE6 CRC64;
     MVAMDPTGPS ESIYNLIPSD WKEPPQPPRY VSIFKATVKD DMQKFKTAMK TMGPAKLEVP
     SPKDFLKKHS KEKILPPKKK FEWNERRKPP VPLRTDHPVM GIQSEKNFIN TNAADVIMGV
     AKKPKPIYVD KRTGDKHDLE TSGLVPKYIN KKDYGVTPEY ICKRNEEVKK AQEEYDNYIQ
     ENLRKAAMKR LSDEEREAVL QGLKKNWEEV HKEFQSLSVF IDSIPKKIRK QKLEEEMKQL
     EHDIGVLEKH KVIYIANKK