AGL16_ARATH
ID AGL16_ARATH Reviewed; 240 AA.
AC A2RVQ5; Q7XYY8; Q9FPP0; Q9M2M4;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Agamous-like MADS-box protein AGL16;
GN Name=AGL16; OrderedLocusNames=At3g57230; ORFNames=F28O9.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, AND GENE
RP FAMILY.
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 24-240 (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta; TISSUE=Leaf, Root, and Stem;
RX PubMed=11115127; DOI=10.1046/j.1365-313x.2000.00891.x;
RA Alvarez-Buylla E.R., Liljegren S.J., Pelaz S., Gold S.E., Burgeff C.,
RA Ditta G.S., Vergara-Silva F., Yanofsky M.F.;
RT "MADS-box gene evolution beyond flowers: expression in pollen, endosperm,
RT guard cells, roots and trichomes.";
RL Plant J. 24:457-466(2000).
RN [6]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [7]
RP INTERACTION WITH AGL15; AGL16; AGL24; AP1; AGL6; AG; AGL1; AGL11; AGL5;
RP SEP3; SEP1; AGL63; AGL14; SOC1 AND AGL21.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [8]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17704216; DOI=10.1105/tpc.107.050377;
RA Kutter C., Schoeb H., Stadler M., Meins F. Jr., Si-Ammour A.;
RT "MicroRNA-mediated regulation of stomatal development in Arabidopsis.";
RL Plant Cell 19:2417-2429(2007).
RN [9]
RP REPRESSION BY MIR824.
RX PubMed=18579782; DOI=10.1073/pnas.0803218105;
RA de Meaux J., Hu J.-Y., Tartler U., Goebel U.;
RT "Structurally different alleles of the ath-MIR824 microRNA precursor are
RT maintained at high frequency in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:8994-8999(2008).
RN [10]
RP INTERACTION WITH AGL63.
RC STRAIN=cv. Columbia; TISSUE=Flower, and Flower bud;
RX PubMed=20598091; DOI=10.1111/j.1365-313x.2010.04290.x;
RA Erdmann R., Gramzow L., Melzer R., Theissen G., Becker A.;
RT "GORDITA (AGL63) is a young paralog of the Arabidopsis thaliana B(sister)
RT MADS box gene ABS (TT16) that has undergone neofunctionalization.";
RL Plant J. 63:914-924(2010).
RN [11]
RP FUNCTION, AND INTERACTION WITH SVP.
RX PubMed=24876250; DOI=10.1105/tpc.114.124685;
RA Hu J.Y., Zhou Y., He F., Dong X., Liu L.Y., Coupland G., Turck F.,
RA de Meaux J.;
RT "miR824-regulated AGAMOUS-LIKE16 contributes to flowering time repression
RT in Arabidopsis.";
RL Plant Cell 26:2024-2037(2014).
CC -!- FUNCTION: Probable transcription factor involved in the regulation of
CC flowering time in long-day photoperiod. Participates in the repression
CC of FT expression and floral transition, by interacting closely with the
CC FLC-SVP pathways (PubMed:24876250). Functions in the satellite
CC meristemoid lineage of stomatal development (PubMed:17704216).
CC {ECO:0000269|PubMed:17704216, ECO:0000269|PubMed:24876250}.
CC -!- SUBUNIT: Homodimer. Interacts with AGL15, AGL24, AP1, AGL6, AG, AGL1,
CC AGL11, AGL5, SEP3, SEP1, AGL63, AGL14, SOC1 and AGL21
CC (PubMed:15805477). Interacts with AGL63 (PubMed:20598091). Interacts
CC with SVP (PubMed:24876250). {ECO:0000269|PubMed:15805477,
CC ECO:0000269|PubMed:20598091, ECO:0000269|PubMed:24876250}.
CC -!- INTERACTION:
CC A2RVQ5; Q9SZJ6: AGL21; NbExp=5; IntAct=EBI-621930, EBI-621986;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2RVQ5-1; Sequence=Displayed;
CC Name=2; Synonyms=AGL16-II;
CC IsoId=A2RVQ5-2; Sequence=VSP_041688;
CC -!- TISSUE SPECIFICITY: Expressed at high levels in leaves, moderate levels
CC in roots, seedlings and stems, and at low levels in flowers, pollen and
CC siliques. Accumulates in leaf guard cells and trichomes. Also present
CC in epidermal cells of roots (PubMed:11115127, PubMed:12837945,
CC PubMed:12949148). Expressed in mature guard cells (PubMed:17704216).
CC {ECO:0000269|PubMed:11115127, ECO:0000269|PubMed:12837945,
CC ECO:0000269|PubMed:12949148, ECO:0000269|PubMed:17704216}.
CC -!- INDUCTION: Repressed by the micro RNA miR824.
CC {ECO:0000269|PubMed:18579782}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB68129.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY141209; AAN52773.1; -; mRNA.
DR EMBL; AL137080; CAB68129.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79629.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79630.2; -; Genomic_DNA.
DR EMBL; CP002686; ANM64245.1; -; Genomic_DNA.
DR EMBL; BT030046; ABN04784.1; -; mRNA.
DR EMBL; AF312662; AAG37899.1; -; mRNA.
DR RefSeq; NP_001319775.1; NM_001339845.1. [A2RVQ5-2]
DR RefSeq; NP_001326287.1; NM_001339847.1. [A2RVQ5-1]
DR RefSeq; NP_191282.2; NM_115583.6. [A2RVQ5-1]
DR AlphaFoldDB; A2RVQ5; -.
DR SMR; A2RVQ5; -.
DR BioGRID; 10206; 43.
DR IntAct; A2RVQ5; 40.
DR STRING; 3702.AT3G57230.1; -.
DR PaxDb; A2RVQ5; -.
DR PRIDE; A2RVQ5; -.
DR ProteomicsDB; 244833; -. [A2RVQ5-1]
DR EnsemblPlants; AT3G57230.1; AT3G57230.1; AT3G57230. [A2RVQ5-1]
DR EnsemblPlants; AT3G57230.2; AT3G57230.2; AT3G57230. [A2RVQ5-2]
DR EnsemblPlants; AT3G57230.4; AT3G57230.4; AT3G57230. [A2RVQ5-1]
DR GeneID; 824890; -.
DR Gramene; AT3G57230.1; AT3G57230.1; AT3G57230. [A2RVQ5-1]
DR Gramene; AT3G57230.2; AT3G57230.2; AT3G57230. [A2RVQ5-2]
DR Gramene; AT3G57230.4; AT3G57230.4; AT3G57230. [A2RVQ5-1]
DR KEGG; ath:AT3G57230; -.
DR Araport; AT3G57230; -.
DR TAIR; locus:2082618; AT3G57230.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_2_0_1; -.
DR InParanoid; A2RVQ5; -.
DR OMA; MNANSEA; -.
DR PhylomeDB; A2RVQ5; -.
DR PRO; PR:A2RVQ5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; A2RVQ5; baseline and differential.
DR Genevisible; A2RVQ5; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010440; P:stomatal lineage progression; IMP:TAIR.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..240
FT /note="Agamous-like MADS-box protein AGL16"
FT /id="PRO_0000412530"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 86..176
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT VAR_SEQ 142..240
FT /note="DQMLIEEIQVLNREGNLVHQENLDLHKKVNLMHQQNMELHEKVSEVEGVKIA
FT NKNSLLTNGLDMRDTSNEHVHLQLSQPQHDHETHSKAIQLNYFSFIA -> VLML (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:12837945"
FT /id="VSP_041688"
SQ SEQUENCE 240 AA; 27428 MW; 96DDECC135221A3B CRC64;
MGRGKIAIKR INNSTSRQVT FSKRRNGLLK KAKELAILCD AEVGVIIFSS TGRLYDFSSS
SMKSVIERYS DAKGETSSEN DPASEIQFWQ KEAAILKRQL HNLQENHRQM MGEELSGLSV
EALQNLENQL ELSLRGVRMK KDQMLIEEIQ VLNREGNLVH QENLDLHKKV NLMHQQNMEL
HEKVSEVEGV KIANKNSLLT NGLDMRDTSN EHVHLQLSQP QHDHETHSKA IQLNYFSFIA
