ENKUR_PIG
ID ENKUR_PIG Reviewed; 256 AA.
AC A0A4X1TZW7; A0A286ZIE6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2019, sequence version 1.
DT 03-AUG-2022, entry version 14.
DE RecName: Full=Enkurin;
GN Name=ENKUR;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Duroc;
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP IDENTIFICATION.
RX PubMed=30723633; DOI=10.7717/peerj.6374;
RA Gilbert D.G.;
RT "Genes of the pig, Sus scrofa, reconstructed with EvidentialGene.";
RL PeerJ 7:E6374-E6374(2019).
RN [3]
RP INTERACTION WITH CFAP45.
RX PubMed=33139725; DOI=10.1038/s41467-020-19113-0;
RA Dougherty G.W., Mizuno K., Noethe-Menchen T., Ikawa Y., Boldt K.,
RA Ta-Shma A., Aprea I., Minegishi K., Pang Y.P., Pennekamp P., Loges N.T.,
RA Raidt J., Hjeij R., Wallmeier J., Mussaffi H., Perles Z., Elpeleg O.,
RA Rabert F., Shiratori H., Letteboer S.J., Horn N., Young S., Struenker T.,
RA Stumme F., Werner C., Olbrich H., Takaoka K., Ide T., Twan W.K.,
RA Biebach L., Grosse-Onnebrink J., Klinkenbusch J.A., Praveen K.,
RA Bracht D.C., Hoeben I.M., Junger K., Guetzlaff J., Cindric S., Aviram M.,
RA Kaiser T., Memari Y., Dzeja P.P., Dworniczak B., Ueffing M., Roepman R.,
RA Bartscherer K., Katsanis N., Davis E.E., Amirav I., Hamada H., Omran H.;
RT "CFAP45 deficiency causes situs abnormalities and asthenospermia by
RT disrupting an axonemal adenine nucleotide homeostasis module.";
RL Nat. Commun. 11:5520-5520(2020).
CC -!- FUNCTION: Adapter that functions to localize a calcium-sensitive signal
CC transduction machinery in sperm to a calcium-permeable ion channel (By
CC similarity). Microtubule inner protein (MIP) part of the dynein-
CC decorated doublet microtubules (DMTs) in cilia axoneme, which is
CC required for motile cilia beating (By similarity).
CC {ECO:0000250|UniProtKB:E1B836, ECO:0000250|UniProtKB:Q6SP97}.
CC -!- SUBUNIT: Binds calmodulin via its IQ domain. Interacts with TRPC1,
CC TRPC2, TRPC5, but not TRPC3 (By similarity). Interacts with CFAP45
CC (PubMed:33139725). {ECO:0000250|UniProtKB:Q6SP97,
CC ECO:0000269|PubMed:33139725}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:E1B836}. Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:Q6SP97}. Note=Sperm acrosomal crescent and
CC flagellar principal piece. {ECO:0000250|UniProtKB:Q6SP97}.
CC -!- DOMAIN: The IQ motif is involved in calmodulin binding.
CC {ECO:0000250|UniProtKB:Q6SP97}.
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DR EMBL; AEMK02000074; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DQIR01123228; HDA78704.1; -; Transcribed_RNA.
DR RefSeq; XP_013845184.1; XM_013989730.1.
DR SMR; A0A4X1TZW7; -.
DR Ensembl; ENSSSCT00000045451; ENSSSCP00000031285; ENSSSCG00000032929.
DR Ensembl; ENSSSCT00005032669; ENSSSCP00005019903; ENSSSCG00005020746.
DR Ensembl; ENSSSCT00005032687; ENSSSCP00005019915; ENSSSCG00005020746.
DR Ensembl; ENSSSCT00015072739; ENSSSCP00015029177; ENSSSCG00015054604.
DR Ensembl; ENSSSCT00025056318; ENSSSCP00025023851; ENSSSCG00025040839.
DR Ensembl; ENSSSCT00030087751; ENSSSCP00030040540; ENSSSCG00030062649.
DR Ensembl; ENSSSCT00035103405; ENSSSCP00035044240; ENSSSCG00035076031.
DR Ensembl; ENSSSCT00035103418; ENSSSCP00035044244; ENSSSCG00035076031.
DR Ensembl; ENSSSCT00040034330; ENSSSCP00040014164; ENSSSCG00040025515.
DR Ensembl; ENSSSCT00045035475; ENSSSCP00045024639; ENSSSCG00045020798.
DR Ensembl; ENSSSCT00050096851; ENSSSCP00050041728; ENSSSCG00050071002.
DR Ensembl; ENSSSCT00055039011; ENSSSCP00055031026; ENSSSCG00055019785.
DR Ensembl; ENSSSCT00060012044; ENSSSCP00060004512; ENSSSCG00060009326.
DR Ensembl; ENSSSCT00065058644; ENSSSCP00065025420; ENSSSCG00065042876.
DR Ensembl; ENSSSCT00070025403; ENSSSCP00070021051; ENSSSCG00070013020.
DR VGNC; VGNC:96174; ENKUR.
DR GeneTree; ENSGT00940000153866; -.
DR OMA; YISTFRP; -.
DR OrthoDB; 1449984at2759; -.
DR Proteomes; UP000008227; Chromosome 10.
DR Proteomes; UP000314985; Chromosome 10.
DR Bgee; ENSSSCG00000032929; Expressed in testis and 47 other tissues.
DR ExpressionAtlas; A0A4X1TZW7; baseline.
DR GO; GO:0097728; C:9+0 motile cilium; IEA:Ensembl.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:Ensembl.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0097228; C:sperm principal piece; IEA:Ensembl.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0017124; F:SH3 domain binding; IEA:UniProtKB-KW.
DR GO; GO:0061966; P:establishment of left/right asymmetry; IEA:Ensembl.
DR GO; GO:0030317; P:flagellated sperm motility; IEA:Ensembl.
DR InterPro; IPR026150; Enkur.
DR InterPro; IPR027012; Enkurin_dom.
DR PANTHER; PTHR21490:SF0; PTHR21490:SF0; 1.
DR Pfam; PF13864; Enkurin; 1.
DR PROSITE; PS51665; ENKURIN; 1.
PE 1: Evidence at protein level;
KW Calmodulin-binding; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Flagellum; Reference proteome; SH3-binding.
FT CHAIN 1..256
FT /note="Enkurin"
FT /id="PRO_0000454205"
FT DOMAIN 160..252
FT /note="Enkurin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01000"
FT DOMAIN 176..187
FT /note="IQ"
FT /evidence="ECO:0000250|UniProtKB:Q8TC29"
FT REGION 48..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 83..89
FT /note="SH3-binding"
FT /evidence="ECO:0000255"
FT COMPBIAS 58..89
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 256 AA; 29891 MW; 8784E8893EC8106C CRC64;
MDPTYSSESI YNLIPSDWKE PPQPPRYISI FKATVKDDMQ KFKTAMKTMG PAKLEVPSPK
DFLKKHSKEK TLPPKKKFDR HEPKKPPVPL RTEHPVMGIQ SEKNFINTNA ANVIMGVAKK
PKPVYVDKRT GDKHDLETSG LVPKYINKKD YGVTPEYICK RNEEVKKAQE EYDNYIQENL
RKAAMKRLSD EEREAVLQGL KKNWEEVHKE FQSLSVFIDS IPKKMRKQKL EEEMKQLEHD
IAVLEKHKII YIANKK
