ID   ENL1_ORYSJ              Reviewed;         987 AA.
AC   A0A0P0WGX7; C7J207;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=SNF2 domain-containing protein ENL1 {ECO:0000305};
DE            EC=3.6.4.- {ECO:0000305};
DE   AltName: Full=Protein ENDOSPERMLESS 1 {ECO:0000303|PubMed:25327517};
GN   Name=ENL1 {ECO:0000303|PubMed:25327517};
GN   OrderedLocusNames=Os04g0692750 {ECO:0000312|EMBL:BAS91802.1},
GN   LOC_Os04g59624 {ECO:0000305};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=25327517; DOI=10.1111/tpj.12705;
RA   Hara T., Katoh H., Ogawa D., Kagaya Y., Sato Y., Kitano H., Nagato Y.,
RA   Ishikawa R., Ono A., Kinoshita T., Takeda S., Hattori T.;
RT   "Rice SNF2 family helicase ENL1 is essential for syncytial endosperm
RT   development.";
RL   Plant J. 81:1-12(2015).
CC   -!- FUNCTION: DNA helicase that acts as an essential component of the
CC       spindle assembly checkpoint (By similarity). Plays an indispensable
CC       role in the development of seed endosperm (PubMed:25327517). Is
CC       required to secure sister chromosome separation during endosperm
CC       syncytial mitosis, which involves extremely rapid free nuclear cycles
CC       (PubMed:25327517). {ECO:0000250|UniProtKB:Q2NKX8,
CC       ECO:0000269|PubMed:25327517}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25327517}.
CC       Chromosome {ECO:0000269|PubMed:25327517}. Note=Localizes to the
CC       cytoplasm during interphase, but moves to the chromosome arms during
CC       mitosis. {ECO:0000269|PubMed:25327517}.
CC   -!- TISSUE SPECIFICITY: Expressed in ovaries, roots, shoots and leaves.
CC       {ECO:0000269|PubMed:25327517}.
CC   -!- DISRUPTION PHENOTYPE: Lack of seed endosperm, alteration of ovary and
CC       embryo shape, reduced root and plant growth, but fertile plants.
CC       {ECO:0000269|PubMed:25327517}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAH92889.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP008210; BAH92889.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014960; BAS91802.1; -; Genomic_DNA.
DR   RefSeq; XP_015637061.1; XM_015781575.1.
DR   AlphaFoldDB; A0A0P0WGX7; -.
DR   SMR; A0A0P0WGX7; -.
DR   STRING; 4530.OS04T0692750-00; -.
DR   PaxDb; A0A0P0WGX7; -.
DR   EnsemblPlants; Os04t0692750-01; Os04t0692750-01; Os04g0692750.
DR   GeneID; 9266414; -.
DR   Gramene; Os04t0692750-01; Os04t0692750-01; Os04g0692750.
DR   eggNOG; KOG0387; Eukaryota.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140658; F:ATP-dependent chromatin remodeler activity; IEA:InterPro.
DR   GO; GO:0015616; F:DNA translocase activity; IBA:GO_Central.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:2000014; P:regulation of endosperm development; IMP:UniProtKB.
DR   GO; GO:0010965; P:regulation of mitotic sister chromatid separation; IMP:UniProtKB.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00176; SNF2-rel_dom; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cell cycle; Cell division; Chromosome; Cytoplasm;
KW   DNA recombination; Helicase; Hydrolase; Mitosis; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..987
FT                   /note="SNF2 domain-containing protein ENL1"
FT                   /id="PRO_0000452398"
FT   DOMAIN          292..466
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          645..801
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          1..172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          224..245
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           417..420
FT                   /note="DEAH box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   COMPBIAS        51..73
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        86..113
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        140..154
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         305..312
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   987 AA;  110498 MW;  27C7AC796E448361 CRC64;
     MASPPPFDIC GDLDDDPTPP APTPLAAPTP NGLNDRLLRL TRTHQRGPSQ NPNPNPNPNP
     KPPPPPPPQE PEPAKVKLAG RRRLCKLSTA GDESAGDDDS IRDILDDLTT RLDSLSVDRP
     TARPRPHVSP LPCALHADPD PSQSQLNDGT KPSSSFVDCD DDDDDAGGAY GGFGVKEEVT
     RKVFKASSSF GGRGNDDKMK AKGAYAFDTV SRKTTTESKA SKFFGDYDDE DDIDQDAENG
     KENHADDVGW EKTEDFKMEP TGTGVTRKPY NLPGRIFNML YPHQREGLRW LWVLHCRGTG
     GILGDDMGLG KTMQVSAFLA GLFHSRLIKR VLVVAPKTLL THWTKELSVV SLKDKIRDYS
     GPNANARNYE LKYAFKEGGI LLTTYDIVRN NFKMIKGNFT NDFDDEEETL WNYVILDEGH
     IIKNPKTQRA QSLFEIPCAH RIVISGTPIQ NNLKEMWALF YFCCPEVLGD KEQFKARYEH
     AIIQGNDKNA TNRQKHIGSN VAKELRERIK PYFLRRMKNE VFLDSGTGED KKLAKKNELI
     IWLKLTSCQR QLYEAFLNSE LVHSSMQGSP LAAITILKKI CDHPLLLTKK AAEGVLEGMD
     AMLNNQEMGM VEKMAMNLAD MAHDDDDVEL QVGQDVSCKL SFMMSLLQNL VSEGHNVLIF
     SQTRKMLNII QEAIILEGYK FLRIDGTTKI SERERIVKDF QEGPGAPIFL LTTQVGGLGL
     TLTKAARVIV VDPAWNPSTD NQSVDRAYRI GQMKDVIVYR LMTSGTIEEK IYKLQVFKGA
     LFRTATEHKE QTRYFSKRDI QELFSLPEQG FDVSLTQKQL QEEHGQQLVM DDSLRKHIQF
     LEQQGIAGVS HHSLLFSKTA ILPTLNDNDG LDSRRAMPMA KHYYKGASSD YVANGAAYAM
     KPKEFIARTY SPNSTSTESP EEIKAKINRL SQTLANTVLV AKLPDRGDKI RRQINELDEK
     LTVIESSPEP LERKGPTEVI CLDDLSV