AGL18_ARATH
ID AGL18_ARATH Reviewed; 256 AA.
AC Q9M2K8; Q1ECG0; Q570M9; Q8LAS6;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Agamous-like MADS-box protein AGL18;
GN Name=AGL18; OrderedLocusNames=At3g57390; ORFNames=F28O9.240;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11115127; DOI=10.1046/j.1365-313x.2000.00891.x;
RA Alvarez-Buylla E.R., Liljegren S.J., Pelaz S., Gold S.E., Burgeff C.,
RA Ditta G.S., Vergara-Silva F., Yanofsky M.F.;
RT "MADS-box gene evolution beyond flowers: expression in pollen, endosperm,
RT guard cells, roots and trichomes.";
RL Plant J. 24:457-466(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [8]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=16028119; DOI=10.1007/s11103-005-4546-3;
RA Lehti-Shiu M.D., Adamczyk B.J., Fernandez D.E.;
RT "Expression of MADS-box genes during the embryonic phase in Arabidopsis.";
RL Plant Mol. Biol. 58:89-107(2005).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=18034896; DOI=10.1186/gb-2007-8-11-r249;
RA Verelst W., Twell D., de Folter S., Immink R., Saedler H., Muenster T.;
RT "MADS-complexes regulate transcriptome dynamics during pollen maturation.";
RL Genome Biol. 8:R249.1-R249.15(2007).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=17521410; DOI=10.1111/j.1365-313x.2007.03105.x;
RA Adamczyk B.J., Lehti-Shiu M.D., Fernandez D.E.;
RT "The MADS domain factors AGL15 and AGL18 act redundantly as repressors of
RT the floral transition in Arabidopsis.";
RL Plant J. 50:1007-1019(2007).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18799658; DOI=10.1104/pp.108.127639;
RA Pouteau S., Carre I., Gaudin V., Ferret V., Lefebvre D., Wilson M.;
RT "Diversification of photoperiodic response patterns in a collection of
RT early-flowering mutants of Arabidopsis.";
RL Plant Physiol. 148:1465-1473(2008).
CC -!- FUNCTION: Probable transcription factor involved in the negative
CC regulation of flowering, probably through the photoperiodic pathway.
CC Prevents premature flowering. Downstream regulator of a subset of the
CC MIKC* MADS-controlled genes required during pollen maturation.
CC {ECO:0000269|PubMed:17521410, ECO:0000269|PubMed:18034896,
CC ECO:0000269|PubMed:18799658}.
CC -!- INTERACTION:
CC Q9M2K8; Q05153: SSRP1; NbExp=3; IntAct=EBI-15197363, EBI-15191543;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9M2K8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9M2K8-2; Sequence=VSP_041689;
CC Name=3;
CC IsoId=Q9M2K8-3; Sequence=VSP_041690, VSP_041691;
CC -!- TISSUE SPECIFICITY: Mostly expressed in pollen, roots, flowers and
CC siliques, and to a lower extent, in stems and leaves. Expressed in the
CC endosperm and in developing male and female gametophytes. Also present
CC in seedlings. {ECO:0000269|PubMed:11115127,
CC ECO:0000269|PubMed:12837945, ECO:0000269|PubMed:12949148,
CC ECO:0000269|PubMed:16028119, ECO:0000269|PubMed:17521410}.
CC -!- DEVELOPMENTAL STAGE: During the reproductive phase, accumulates in
CC immature buds and at the base of the floral organs, and in the
CC receptacle, ovules, anther filaments, and stigma and style of open
CC flowers. Later observed in sporogenous tissue of anthers. During male
CC gametogenesis, expressed in the microspores before they separate from
CC each other. Later present at high levels within pollen grains up to
CC stage 13 of flower development, when anthers dehisce. During carpel
CC development, first detected in developing ovules. After fertilization,
CC confined to globular structures or nodules of proliferating free
CC nuclear endosperm required for embryo development. Disappears from the
CC endosperm at to the heart stage of embryo development, when very little
CC nuclear endosperm remains. Never detected in developing embryos at any
CC stage. In young seedlings, present everywhere except in a portion of
CC the hypocotyl and in newly emerging leaves.
CC {ECO:0000269|PubMed:11115127, ECO:0000269|PubMed:17521410}.
CC -!- DISRUPTION PHENOTYPE: Altered expression of MIKC* MADS-controlled genes
CC during pollen maturation. Early flowering under short-days conditions
CC (SD) when combined with AGL15 disruption. {ECO:0000269|PubMed:17521410,
CC ECO:0000269|PubMed:18034896, ECO:0000269|PubMed:18799658}.
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DR EMBL; AF312663; AAG37900.1; -; mRNA.
DR EMBL; AL137080; CAB68145.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79649.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79650.1; -; Genomic_DNA.
DR EMBL; AK220679; BAD93735.1; -; mRNA.
DR EMBL; AK222220; BAD95389.1; -; mRNA.
DR EMBL; BT025774; ABF83664.1; -; mRNA.
DR EMBL; AY087639; AAM65178.1; -; mRNA.
DR PIR; T45817; T45817.
DR RefSeq; NP_191298.1; NM_115599.4. [Q9M2K8-1]
DR RefSeq; NP_974450.1; NM_202721.1. [Q9M2K8-2]
DR AlphaFoldDB; Q9M2K8; -.
DR SMR; Q9M2K8; -.
DR BioGRID; 10222; 17.
DR IntAct; Q9M2K8; 9.
DR STRING; 3702.AT3G57390.1; -.
DR PaxDb; Q9M2K8; -.
DR PRIDE; Q9M2K8; -.
DR ProteomicsDB; 244881; -. [Q9M2K8-1]
DR EnsemblPlants; AT3G57390.1; AT3G57390.1; AT3G57390. [Q9M2K8-1]
DR EnsemblPlants; AT3G57390.2; AT3G57390.2; AT3G57390. [Q9M2K8-2]
DR GeneID; 824906; -.
DR Gramene; AT3G57390.1; AT3G57390.1; AT3G57390. [Q9M2K8-1]
DR Gramene; AT3G57390.2; AT3G57390.2; AT3G57390. [Q9M2K8-2]
DR KEGG; ath:AT3G57390; -.
DR Araport; AT3G57390; -.
DR TAIR; locus:2082613; AT3G57390.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_2_1; -.
DR InParanoid; Q9M2K8; -.
DR PhylomeDB; Q9M2K8; -.
DR PRO; PR:Q9M2K8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M2K8; baseline and differential.
DR Genevisible; Q9M2K8; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0009910; P:negative regulation of flower development; IMP:UniProtKB.
DR GO; GO:0048577; P:negative regulation of short-day photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..256
FT /note="Agamous-like MADS-box protein AGL18"
FT /id="PRO_0000412531"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 94..184
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT REGION 179..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..61
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_041689"
FT VAR_SEQ 150..154
FT /note="TQILL -> VKTKY (in isoform 3)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_041690"
FT VAR_SEQ 155..256
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_041691"
FT CONFLICT 106
FT /note="G -> V (in Ref. 6; AAM65178)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="L -> F (in Ref. 6; AAM65178)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 256 AA; 28874 MW; ECF106A22F6F8627 CRC64;
MGRGRIEIKK IENINSRQVT FSKRRNGLIK KAKELSILCD AEVALIIFSS TGKIYDFSSV
CMEQILSRYG YTTASTEHKQ QREHQLLICA SHGNEAVLRN DDSMKGELER LQLAIERLKG
KELEGMSFPD LISLENQLNE SLHSVKDQKT QILLNQIERS RIQEKKALEE NQILRKQVEM
LGRGSGPKVL NERPQDSSPE ADPESSSSEE DENDNEEHHS DTSLQLGLSS TGYCTKRKKP
KIELVCDNSG SQVASD
