AGL24_ARATH
ID AGL24_ARATH Reviewed; 220 AA.
AC O82794;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=MADS-box protein AGL24;
DE AltName: Full=Protein AGAMOUS-LIKE 24;
GN Name=AGL24; OrderedLocusNames=At4g24540; ORFNames=F22K18.260;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Kohalmi S.E., Schorr P., Nowak J., Reader L.J., Crosby W.L.;
RT "The Product of AGAMOUS Interacts with Select Members of the MADS-Box
RT Family of Proteins in Arabidopsis thaliana.";
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=12451184; DOI=10.1073/pnas.212624599;
RA Yu H., Xu Y., Tan E.L., Kumar P.P.;
RT "AGAMOUS-LIKE 24, a dosage-dependent mediator of the flowering signals.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16336-16341(2002).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH IMK3,
RP AND PHOSPHORYLATION BY IMK3.
RX PubMed=12881501; DOI=10.1093/pcp/pcg092;
RA Fujita H., Takemura M., Tani E., Nemoto K., Yokota A., Kohchi T.;
RT "An Arabidopsis MADS-box protein, AGL24, is specifically bound to and
RT phosphorylated by meristematic receptor-like kinase (MRLK).";
RL Plant Cell Physiol. 44:735-742(2003).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY VERNALIZATION.
RX PubMed=12609028; DOI=10.1046/j.1365-313x.2003.01671.x;
RA Michaels S.D., Ditta G., Gustafson-Brown C., Pelaz S., Yanofsky M.,
RA Amasino R.M.;
RT "AGL24 acts as a promoter of flowering in Arabidopsis and is positively
RT regulated by vernalization.";
RL Plant J. 33:867-874(2003).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION BY AP1 AND LFY.
RX PubMed=14716314; DOI=10.1038/ng1286;
RA Yu H., Ito T., Wellmer F., Meyerowitz E.M.;
RT "Repression of AGAMOUS-LIKE 24 is a crucial step in promoting flower
RT development.";
RL Nat. Genet. 36:157-161(2004).
RN [10]
RP INTERACTION WITH AGL15 AND AGL16.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [11]
RP FUNCTION, AND INTERACTION WITH AP1; SEU AND LUG.
RX PubMed=16679456; DOI=10.1105/tpc.106.041798;
RA Gregis V., Sessa A., Colombo L., Kater M.M.;
RT "AGL24, SHORT VEGETATIVE PHASE, and APETALA1 redundantly control AGAMOUS
RT during early stages of flower development in Arabidopsis.";
RL Plant Cell 18:1373-1382(2006).
RN [12]
RP INDUCTION BY AP1.
RX PubMed=17428825; DOI=10.1242/dev.003103;
RA Liu C., Zhou J., Bracha-Drori K., Yalovsky S., Ito T., Yu H.;
RT "Specification of Arabidopsis floral meristem identity by repression of
RT flowering time genes.";
RL Development 134:1901-1910(2007).
RN [13]
RP FUNCTION, AND INDUCTION BY SOC1.
RX PubMed=18339670; DOI=10.1242/dev.020255;
RA Liu C., Chen H., Er H.L., Soo H.M., Kumar P.P., Han J.-H., Liou Y.C.,
RA Yu H.;
RT "Direct interaction of AGL24 and SOC1 integrates flowering signals in
RT Arabidopsis.";
RL Development 135:1481-1491(2008).
RN [14]
RP REVIEW.
RX PubMed=18390846; DOI=10.1093/jxb/ern070;
RA Alexandre C.M., Hennig L.;
RT "FLC or not FLC: the other side of vernalization.";
RL J. Exp. Bot. 59:1127-1135(2008).
RN [15]
RP FUNCTION, INTERACTION WITH SOC1, AND SUBCELLULAR LOCATION.
RX PubMed=18466303; DOI=10.1111/j.1365-313x.2008.03552.x;
RA Lee J., Oh M., Park H., Lee I.;
RT "SOC1 translocated to the nucleus by interaction with AGL24 directly
RT regulates leafy.";
RL Plant J. 55:832-843(2008).
RN [16]
RP FUNCTION, AND INDUCTION.
RX PubMed=18694458; DOI=10.1111/j.1365-313x.2008.03648.x;
RA Gregis V., Sessa A., Colombo L., Kater M.M.;
RT "AGAMOUS-LIKE24 and SHORT VEGETATIVE PHASE determine floral meristem
RT identity in Arabidopsis.";
RL Plant J. 56:891-902(2008).
RN [17]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=19656343; DOI=10.1111/j.1365-313x.2009.03985.x;
RA Gregis V., Sessa A., Dorca-Fornell C., Kater M.M.;
RT "The Arabidopsis floral meristem identity genes AP1, AGL24 and SVP directly
RT repress class B and C floral homeotic genes.";
RL Plant J. 60:626-637(2009).
CC -!- FUNCTION: Transcription activator that mediates floral transition in
CC response to vernalization. Promotes inflorescence fate in apical
CC meristems. Acts in a dosage-dependent manner. Probably involved in the
CC transduction of RLK-mediated signaling (e.g. IMK3 pathway). Together
CC with AP1 and SVP, controls the identity of the floral meristem and
CC regulates expression of class B, C and E genes. When associated with
CC SOC1, mediates effect of gibberellins on flowering under short-day
CC conditions, and regulates the expression of LEAFY (LFY), which links
CC floral induction and floral development. Confers inflorescence
CC characteristics to floral primordia and early flowering.
CC {ECO:0000269|PubMed:12451184, ECO:0000269|PubMed:12609028,
CC ECO:0000269|PubMed:12881501, ECO:0000269|PubMed:14716314,
CC ECO:0000269|PubMed:16679456, ECO:0000269|PubMed:18339670,
CC ECO:0000269|PubMed:18466303, ECO:0000269|PubMed:18694458,
CC ECO:0000269|PubMed:19656343}.
CC -!- SUBUNIT: Interacts with IMK3/MRLK. Forms a homodimer and heterodimer
CC with SOC1, AP1 and SVP through MADS-box domain. Interacts with the SEU-
CC LUG corepressor complex when complexed to AP1. Interacts with AGL15 and
CC AGL16. {ECO:0000269|PubMed:12881501, ECO:0000269|PubMed:15805477,
CC ECO:0000269|PubMed:16679456, ECO:0000269|PubMed:18466303}.
CC -!- INTERACTION:
CC O82794; Q38838: AGL14; NbExp=6; IntAct=EBI-592083, EBI-622067;
CC O82794; Q9SZJ6: AGL21; NbExp=4; IntAct=EBI-592083, EBI-621986;
CC O82794; Q38876: AGL8; NbExp=3; IntAct=EBI-592083, EBI-621912;
CC O82794; P35631: AP1; NbExp=4; IntAct=EBI-592083, EBI-592003;
CC O82794; Q9SL42: PIN1; NbExp=4; IntAct=EBI-592083, EBI-2618990;
CC O82794; P29382: SEP1; NbExp=3; IntAct=EBI-592083, EBI-632935;
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Translocation from the
CC cytoplasm to the nucleus in the presence of IMK3.
CC -!- TISSUE SPECIFICITY: Mostly expressed in shoot apical meristems,
CC including floral meristems. Also detected in stems, seedlings, leaves,
CC flowers and siliques, and, to a lower extent, in roots.
CC {ECO:0000269|PubMed:12451184, ECO:0000269|PubMed:12609028,
CC ECO:0000269|PubMed:12881501, ECO:0000269|PubMed:19656343}.
CC -!- DEVELOPMENTAL STAGE: During vegetative phase, expressed in the entire
CC shoot apical meristem and in emerging leaf primordia. During floral
CC transition, gradually detectable in the transitional shoot apex and
CC young cauline leaves. Detected in the inflorescence meristem. Present
CC throughout the tunica (superficial layers) of the floral meristem
CC during early stages of flower development. Later disappears prior to
CC emergence of sepal primordia. At later stages of floral development,
CC weakly expressed in the distal parts of stamens and carpels. Then
CC restricted to pollen and the adaxial surface of the gynoecium.
CC {ECO:0000269|PubMed:12451184, ECO:0000269|PubMed:14716314,
CC ECO:0000269|PubMed:19656343}.
CC -!- INDUCTION: Induced by vernalization in a FLC-independent manner.
CC Repressed by the floral homeotic genes AP1, LFY and SEP3 in emerging
CC floral meristems to establish a floral identity and prevent
CC inflorescence fate. Up-regulated at the shoot apex by SOC1.
CC {ECO:0000269|PubMed:12609028, ECO:0000269|PubMed:14716314,
CC ECO:0000269|PubMed:17428825, ECO:0000269|PubMed:18339670,
CC ECO:0000269|PubMed:18694458}.
CC -!- PTM: Phosphorylated by IMK3. Induced by vernalization.
CC {ECO:0000269|PubMed:12881501}.
CC -!- DISRUPTION PHENOTYPE: Delayed flowering. {ECO:0000269|PubMed:12451184}.
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DR EMBL; AF005158; AAC63139.1; -; mRNA.
DR EMBL; AF005159; AAC63140.1; -; Genomic_DNA.
DR EMBL; AL035356; CAA23009.1; -; Genomic_DNA.
DR EMBL; AL161561; CAB79364.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84922.1; -; Genomic_DNA.
DR EMBL; BT025171; ABE77409.1; -; mRNA.
DR EMBL; AB493697; BAH30535.1; -; mRNA.
DR PIR; T05580; T05580.
DR PIR; T51827; T51827.
DR RefSeq; NP_194185.1; NM_118587.6.
DR AlphaFoldDB; O82794; -.
DR SMR; O82794; -.
DR BioGRID; 13845; 19.
DR ELM; O82794; -.
DR IntAct; O82794; 15.
DR STRING; 3702.AT4G24540.1; -.
DR PaxDb; O82794; -.
DR PRIDE; O82794; -.
DR EnsemblPlants; AT4G24540.1; AT4G24540.1; AT4G24540.
DR GeneID; 828556; -.
DR Gramene; AT4G24540.1; AT4G24540.1; AT4G24540.
DR KEGG; ath:AT4G24540; -.
DR Araport; AT4G24540; -.
DR TAIR; locus:2121924; AT4G24540.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_14_1_1; -.
DR InParanoid; O82794; -.
DR OMA; TTMEMAK; -.
DR OrthoDB; 1301596at2759; -.
DR PhylomeDB; O82794; -.
DR PRO; PR:O82794; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O82794; baseline and differential.
DR Genevisible; O82794; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:TAIR.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:TAIR.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IPI:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0048438; P:floral whorl development; IGI:TAIR.
DR GO; GO:0010077; P:maintenance of inflorescence meristem identity; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEP:UniProtKB.
DR GO; GO:0010220; P:positive regulation of vernalization response; IEP:UniProtKB.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009739; P:response to gibberellin; IEP:UniProtKB.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Developmental protein; Differentiation; DNA-binding;
KW Flowering; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..220
FT /note="MADS-box protein AGL24"
FT /id="PRO_0000392926"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 87..177
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT REGION 190..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 190..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 220 AA; 25064 MW; C58CB708B3AC99EB CRC64;
MAREKIRIKK IDNITARQVT FSKRRRGIFK KADELSVLCD ADVALIIFSA TGKLFEFSSS
RMRDILGRYS LHASNINKLM DPPSTHLRLE NCNLSRLSKE VEDKTKQLRK LRGEDLDGLN
LEELQRLEKL LESGLSRVSE KKGECVMSQI FSLEKRGSEL VDENKRLRDK LETLERAKLT
TLKEALETES VTTNVSSYDS GTPLEDDSDT SLKLGLPSWE
