ID   ENO4_XENTR              Reviewed;         574 AA.
AC   B0BM20;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Enolase 4;
DE            EC=4.2.1.11 {ECO:0000250|UniProtKB:Q8C042};
DE   AltName: Full=2-phospho-D-glycerate hydro-lyase;
GN   Name=eno4;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:10164, ChEBI:CHEBI:15377, ChEBI:CHEBI:58289,
CC         ChEBI:CHEBI:58702; EC=4.2.1.11;
CC         Evidence={ECO:0000250|UniProtKB:Q8C042};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 4/5. {ECO:0000250|UniProtKB:Q8C042}.
CC   -!- SIMILARITY: Belongs to the enolase family. {ECO:0000305}.
CC   -!- CAUTION: Although it belongs to the enolase family, Leu-332 is present
CC       instead of the conserved Glu which is expected to be an active site
CC       residue. {ECO:0000305}.
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DR   EMBL; BC158253; AAI58254.1; -; mRNA.
DR   RefSeq; NP_001119992.1; NM_001126520.1.
DR   AlphaFoldDB; B0BM20; -.
DR   SMR; B0BM20; -.
DR   STRING; 8364.ENSXETP00000031321; -.
DR   GeneID; 100144948; -.
DR   KEGG; xtr:100144948; -.
DR   CTD; 387712; -.
DR   Xenbase; XB-GENE-1009720; eno4.
DR   eggNOG; KOG2670; Eukaryota.
DR   InParanoid; B0BM20; -.
DR   OrthoDB; 1097401at2759; -.
DR   UniPathway; UPA00109; UER00187.
DR   Proteomes; UP000008143; Chromosome 7.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0000015; C:phosphopyruvate hydratase complex; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0004634; F:phosphopyruvate hydratase activity; IBA:GO_Central.
DR   GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR   Gene3D; 3.20.20.120; -; 1.
DR   Gene3D; 3.30.390.10; -; 1.
DR   InterPro; IPR000941; Enolase.
DR   InterPro; IPR036849; Enolase-like_C_sf.
DR   InterPro; IPR029017; Enolase-like_N.
DR   InterPro; IPR020810; Enolase_C.
DR   InterPro; IPR020811; Enolase_N.
DR   PANTHER; PTHR11902; PTHR11902; 1.
DR   Pfam; PF00113; Enolase_C; 1.
DR   SMART; SM01192; Enolase_C; 1.
DR   SMART; SM01193; Enolase_N; 1.
DR   SUPFAM; SSF51604; SSF51604; 1.
DR   SUPFAM; SSF54826; SSF54826; 1.
PE   2: Evidence at transcript level;
KW   Glycolysis; Lyase; Reference proteome.
FT   CHAIN           1..574
FT                   /note="Enolase 4"
FT                   /id="PRO_0000348458"
FT   REGION          165..221
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        467
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         287
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         518
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   574 AA;  62651 MW;  67D536E7B27C74C2 CRC64;
     MSYRAGDAAR RARERYELKQ AAAEFYRKLS VTERLEEALN STFCLGPEDV YGHLANYFAQ
     FSKPPTICQI RGRKVLDGTG EPTVEAEVFC TVKNMDKRIC SSVISAASEH PKASKGPEQE
     SNHSADIAIQ WLNDLSPKLR GMSPDEQNKI DQLLSDFYQP KIEEEKERRQ MEREASPMPL
     QPEPSPVTSP APGKKKGSGK GKKAAVVEKP IPPEETPEAV VPGSPAIGAL SLAVAKASSV
     LSKTPLYLHI RALRNEKLPT EFFMPTPMIS ILSCGTSSPG KLNLMKEVLI IPQTGLTVQQ
     SLDMALMLQN QIVKQINAAS KTGPAIKNVS PLGCMLIGGD RIEQPLDLIC EACQHVGLEL
     GTNLYLAINC AAHELMDYNK GKYEVLSGTF KSPDEMIDLY VDLINRQPAI LALLDPLRKE
     DTVQWESLAK ALGSKCYLFA DAASKPVCKL LESGSMNSPP CSGTVIKHTN EITISQLLGV
     FKLIEGENRV AVLGCPYKES VGDSTADLAV GLGARFVKLG GLLRGERTTK YNRLLAIEDE
     LTQAGALGFW TKNEFPVLCE VQNQPGPQET PETQ