AGL27_ARATH
ID AGL27_ARATH Reviewed; 196 AA.
AC Q9AT76; O49291; Q7XYY7; Q9FPN8; Q9FPN9;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Agamous-like MADS-box protein AGL27;
DE AltName: Full=MADS box FLC1-like nuclear protein;
DE AltName: Full=Protein FLOWERING LOCUS M;
DE AltName: Full=Protein MADS AFFECTING FLOWERING 1;
GN Name=AGL27; Synonyms=FK1, FLM, MAF1; OrderedLocusNames=At1g77080;
GN ORFNames=F22K20.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=11115127; DOI=10.1046/j.1365-313x.2000.00891.x;
RA Alvarez-Buylla E.R., Liljegren S.J., Pelaz S., Gold S.E., Burgeff C.,
RA Ditta G.S., Vergara-Silva F., Yanofsky M.F.;
RT "MADS-box gene evolution beyond flowers: expression in pollen, endosperm,
RT guard cells, roots and trichomes.";
RL Plant J. 24:457-466(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, AND
RP REPRESSION BY VERNALIZATION.
RC STRAIN=cv. Columbia;
RX PubMed=11351076; DOI=10.1104/pp.126.1.122;
RA Ratcliffe O.J., Nadzan G.C., Reuber T.L., Riechmann J.L.;
RT "Regulation of flowering in Arabidopsis by an FLC homologue.";
RL Plant Physiol. 126:122-132(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY, AND GENE
RP FAMILY.
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Col-2;
RA Million T., Sheldon C.C., Helliwell C.A., Dennis E.S., Peacock W.J.;
RT "The control of flowering time by FLC orthologues in Brassica napus.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [10]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [11]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=14558654; DOI=10.1023/a:1025426920923;
RA Scortecci K., Michaels S.D., Amasino R.M.;
RT "Genetic interactions between FLM and other flowering-time genes in
RT Arabidopsis thaliana.";
RL Plant Mol. Biol. 52:915-922(2003).
RN [13]
RP INDUCTION BY ELF7 AND ELF8.
RX PubMed=15520273; DOI=10.1101/gad.1244504;
RA He Y., Doyle M.R., Amasino R.M.;
RT "PAF1-complex-mediated histone methylation of FLOWERING LOCUS C chromatin
RT is required for the vernalization-responsive, winter-annual habit in
RT Arabidopsis.";
RL Genes Dev. 18:2774-2784(2004).
RN [14]
RP INTERACTION WITH AGL39; AGL97 AND AGL74.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [15]
RP INDUCTION BY HUA2.
RX PubMed=15659097; DOI=10.1111/j.1365-313x.2004.02300.x;
RA Doyle M.R., Bizzell C.M., Keller M.R., Michaels S.D., Song J., Noh Y.-S.,
RA Amasino R.M.;
RT "HUA2 is required for the expression of floral repressors in Arabidopsis
RT thaliana.";
RL Plant J. 41:376-385(2005).
RN [16]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Col-3, cv. Col-5, and cv. Nd-1;
RX PubMed=15695584; DOI=10.1073/pnas.0409474102;
RA Werner J.D., Borevitz J.O., Warthmann N., Trainer G.T., Ecker J.R.,
RA Chory J., Weigel D.;
RT "Quantitative trait locus mapping and DNA array hybridization identify an
RT FLM deletion as a cause for natural flowering-time variation.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:2460-2465(2005).
RN [17]
RP REPRESSION BY VIL1.
RX PubMed=17114575; DOI=10.1101/gad.1493306;
RA Sung S., Schmitz R.J., Amasino R.M.;
RT "A PHD finger protein involved in both the vernalization and photoperiod
RT pathways in Arabidopsis.";
RL Genes Dev. 20:3244-3248(2006).
RN [18]
RP REVIEW.
RX PubMed=18390846; DOI=10.1093/jxb/ern070;
RA Alexandre C.M., Hennig L.;
RT "FLC or not FLC: the other side of vernalization.";
RL J. Exp. Bot. 59:1127-1135(2008).
RN [19]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18799658; DOI=10.1104/pp.108.127639;
RA Pouteau S., Carre I., Gaudin V., Ferret V., Lefebvre D., Wilson M.;
RT "Diversification of photoperiodic response patterns in a collection of
RT early-flowering mutants of Arabidopsis.";
RL Plant Physiol. 148:1465-1473(2008).
CC -!- FUNCTION: Probable transcription factor involved in the negative
CC regulation of flowering time in both long and short days, probably
CC through the photoperiodic and vernalization pathways. Prevents
CC premature flowering. {ECO:0000269|PubMed:11351076,
CC ECO:0000269|PubMed:14558654, ECO:0000269|PubMed:15695584,
CC ECO:0000269|PubMed:18799658}.
CC -!- SUBUNIT: Interacts with AGL39, AGL97 and AGL74.
CC {ECO:0000269|PubMed:15805477}.
CC -!- INTERACTION:
CC Q9AT76-1; Q9FVC1: SVP; NbExp=7; IntAct=EBI-16074648, EBI-592058;
CC Q9AT76-1; Q2NJQ2: AYWB_224; Xeno; NbExp=3; IntAct=EBI-16074648, EBI-16100490;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9AT76-1; Sequence=Displayed;
CC Name=2; Synonyms=AGL27-I;
CC IsoId=Q9AT76-2; Sequence=VSP_041696, VSP_041698;
CC Name=3; Synonyms=AGL27-II;
CC IsoId=Q9AT76-3; Sequence=VSP_041693;
CC Name=4;
CC IsoId=Q9AT76-4; Sequence=VSP_041692, VSP_041694, VSP_041695,
CC VSP_041697;
CC -!- TISSUE SPECIFICITY: Expressed in most plant tissues, embryo, seedlings,
CC roots, leaves, stems, inflorescence, pollen, siliques and flowers.
CC {ECO:0000269|PubMed:11115127, ECO:0000269|PubMed:11351076,
CC ECO:0000269|PubMed:12837945, ECO:0000269|PubMed:12949148}.
CC -!- INDUCTION: Slightly repressed by vernalization. Negatively regulated at
CC the chromatin level by VIL1 through the photoperiod and vernalization
CC pathways. Requires EARLY FLOWERING 7 (ELF7) and ELF8 to be expressed.
CC Up-regulated by HUA2. {ECO:0000269|PubMed:11351076,
CC ECO:0000269|PubMed:15520273, ECO:0000269|PubMed:15659097,
CC ECO:0000269|PubMed:17114575}.
CC -!- DISRUPTION PHENOTYPE: Suppress the late-flowering phenotype of
CC photoperiod-pathway mutants. Affects natural variation in flowering
CC behavior in both long and short days. {ECO:0000269|PubMed:14558654,
CC ECO:0000269|PubMed:15695584, ECO:0000269|PubMed:18799658}.
CC -!- MISCELLANEOUS: The early flowering of cv. Nd-1 (especially in short
CC days) is due to a deletion of AGL27. This deletion is associated with
CC the QTL 'FLOWERING 1' (FLW1) (PubMed:15695584).
CC {ECO:0000305|PubMed:15695584}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC00628.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF312665; AAG37902.1; -; mRNA.
DR EMBL; AF312666; AAG37903.1; -; mRNA.
DR EMBL; AF342808; AAK37527.1; -; mRNA.
DR EMBL; AY141210; AAN52774.1; -; mRNA.
DR EMBL; AY034083; AAK54440.1; -; mRNA.
DR EMBL; AC002291; AAC00628.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35929.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35930.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35931.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35932.1; -; Genomic_DNA.
DR EMBL; AK117577; BAC42235.1; -; mRNA.
DR EMBL; BT004598; AAO42844.1; -; mRNA.
DR EMBL; AK175247; BAD43010.1; -; mRNA.
DR EMBL; AK176270; BAD44033.1; -; mRNA.
DR EMBL; AK227948; BAE99916.1; -; mRNA.
DR EMBL; AY088709; AAM67028.1; -; mRNA.
DR PIR; F96799; F96799.
DR RefSeq; NP_177833.3; NM_106358.4. [Q9AT76-1]
DR RefSeq; NP_850979.1; NM_180648.3. [Q9AT76-3]
DR RefSeq; NP_850980.2; NM_180649.4. [Q9AT76-4]
DR RefSeq; NP_974160.2; NM_202431.2. [Q9AT76-2]
DR AlphaFoldDB; Q9AT76; -.
DR SMR; Q9AT76; -.
DR BioGRID; 29261; 15.
DR DIP; DIP-33758N; -.
DR IntAct; Q9AT76; 25.
DR STRING; 3702.AT1G77080.4; -.
DR PaxDb; Q9AT76; -.
DR PRIDE; Q9AT76; -.
DR EnsemblPlants; AT1G77080.2; AT1G77080.2; AT1G77080. [Q9AT76-3]
DR EnsemblPlants; AT1G77080.3; AT1G77080.3; AT1G77080. [Q9AT76-4]
DR EnsemblPlants; AT1G77080.4; AT1G77080.4; AT1G77080. [Q9AT76-1]
DR EnsemblPlants; AT1G77080.5; AT1G77080.5; AT1G77080. [Q9AT76-2]
DR GeneID; 844042; -.
DR Gramene; AT1G77080.2; AT1G77080.2; AT1G77080. [Q9AT76-3]
DR Gramene; AT1G77080.3; AT1G77080.3; AT1G77080. [Q9AT76-4]
DR Gramene; AT1G77080.4; AT1G77080.4; AT1G77080. [Q9AT76-1]
DR Gramene; AT1G77080.5; AT1G77080.5; AT1G77080. [Q9AT76-2]
DR KEGG; ath:AT1G77080; -.
DR Araport; AT1G77080; -.
DR TAIR; locus:2025282; AT1G77080.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_4_1; -.
DR InParanoid; Q9AT76; -.
DR OMA; REDQQSM; -.
DR PhylomeDB; Q9AT76; -.
DR PRO; PR:Q9AT76; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9AT76; baseline and differential.
DR Genevisible; Q9AT76; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010582; P:floral meristem determinacy; IBA:GO_Central.
DR GO; GO:0009910; P:negative regulation of flower development; IBA:GO_Central.
DR GO; GO:0048573; P:photoperiodism, flowering; IMP:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:TAIR.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..196
FT /note="Agamous-like MADS-box protein AGL27"
FT /id="PRO_0000412532"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 80..170
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT REGION 175..196
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..62
FT /note="MGRRKIEIKRIENKSSRQVTFSKRRNGLIDKARQLSILCESSVAVVVVSASG
FT KLYDSSSGDD -> MHFCFISS (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12837945"
FT /id="VSP_041692"
FT VAR_SEQ 62..80
FT /note="DISKIIDRYEIQHADELRA -> EIEALFKPEKPQCFE (in isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:11115127"
FT /id="VSP_041693"
FT VAR_SEQ 101
FT /note="Q -> QRLAVRHIFLPSSSDKKNVFFLLSTCEY (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12837945"
FT /id="VSP_041694"
FT VAR_SEQ 150..168
FT /note="EKLLREENQVLASQMGKNT -> VSALVFIFDKGHILGYDDS (in
FT isoform 4)"
FT /evidence="ECO:0000303|PubMed:12837945"
FT /id="VSP_041695"
FT VAR_SEQ 164..173
FT /note="MGKNTLLATD -> LSEKKGMSHR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11115127"
FT /id="VSP_041696"
FT VAR_SEQ 169..196
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12837945"
FT /id="VSP_041697"
FT VAR_SEQ 174..196
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11115127"
FT /id="VSP_041698"
SQ SEQUENCE 196 AA; 22119 MW; 8A9EEEE7085236DD CRC64;
MGRRKIEIKR IENKSSRQVT FSKRRNGLID KARQLSILCE SSVAVVVVSA SGKLYDSSSG
DDISKIIDRY EIQHADELRA LDLEEKIQNY LPHKELLETV QSKLEEPNVD NVSVDSLISL
EEQLETALSV SRARKAELMM EYIESLKEKE KLLREENQVL ASQMGKNTLL ATDDERGMFP
GSSSGNKIPE TLPLLN
