ENOL1_ARATH
ID ENOL1_ARATH Reviewed; 953 AA.
AC F4JF14; Q9M298;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Protein ENHANCER OF LHP1 1 {ECO:0000303|PubMed:28428341};
GN Name=EOL1 {ECO:0000303|PubMed:28428341};
GN OrderedLocusNames=At3g42660 {ECO:0000312|Araport:AT3G42660};
GN ORFNames=T12K4_110 {ECO:0000312|EMBL:CAB86449.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INTERACTION WITH
RP EZA1/SWN; LHP1; SLD5 AND CLF, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND
RP INDUCTION BY AUXIN.
RC STRAIN=cv. Columbia;
RX PubMed=28428341; DOI=10.1073/pnas.1620955114;
RA Zhou Y., Tergemina E., Cui H., Foerderer A., Hartwig B.,
RA Velikkakam James G., Schneeberger K., Turck F.;
RT "Ctf4-related protein recruits LHP1-PRC2 to maintain H3K27me3 levels in
RT dividing cells in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:4833-4838(2017).
CC -!- FUNCTION: Participates in maintaining the H3K27me3 mark at target genes
CC by interacting with LHP1-PRC2 complexes during replication, thus
CC contributing to H3K27me3 inheritance. {ECO:0000269|PubMed:28428341}.
CC -!- SUBUNIT: Interacts with EZA1/SWN, LHP1, SLD5 and CLF in the nucleus.
CC {ECO:0000269|PubMed:28428341}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:28428341}.
CC -!- TISSUE SPECIFICITY: Expressed in root meristematic zones, initiating
CC lateral roots, young leaves and the shoot apex.
CC {ECO:0000269|PubMed:28428341}.
CC -!- DEVELOPMENTAL STAGE: Mainly expressed in dividing cells such as primary
CC roots of seedlings (within the meristematic zone but not in the
CC differentiation zone), lateral roots primordia, developiong leaves and
CC shoot apices. {ECO:0000269|PubMed:28428341}.
CC -!- INDUCTION: Induced by the auxin analog 2,4-D.
CC {ECO:0000269|PubMed:28428341}.
CC -!- DISRUPTION PHENOTYPE: Enhances phenotypes of the lhp1-3 mutant, thus
CC leading to reduced plant size, early flowering in all photoperiod
CC conditions and altered leaf morphology, associated with an increased
CC expression of H3K27me3-positive genes. {ECO:0000269|PubMed:28428341}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB86449.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL138640; CAB86449.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77744.1; -; Genomic_DNA.
DR PIR; T47324; T47324.
DR RefSeq; NP_189852.2; NM_114133.2.
DR AlphaFoldDB; F4JF14; -.
DR SMR; F4JF14; -.
DR STRING; 3702.AT3G42660.1; -.
DR iPTMnet; F4JF14; -.
DR PaxDb; F4JF14; -.
DR PRIDE; F4JF14; -.
DR ProteomicsDB; 195632; -.
DR EnsemblPlants; AT3G42660.1; AT3G42660.1; AT3G42660.
DR GeneID; 823286; -.
DR Gramene; AT3G42660.1; AT3G42660.1; AT3G42660.
DR KEGG; ath:AT3G42660; -.
DR Araport; AT3G42660; -.
DR TAIR; locus:2096489; AT3G42660.
DR eggNOG; KOG1274; Eukaryota.
DR HOGENOM; CLU_004219_4_0_1; -.
DR InParanoid; F4JF14; -.
DR OMA; NAWFPIC; -.
DR OrthoDB; 155530at2759; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JF14; baseline and differential.
DR GO; GO:0043596; C:nuclear replication fork; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0061087; P:positive regulation of histone H3-K27 methylation; IGI:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR022100; Mcl1_mid.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF12341; Mcl1_mid; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..953
FT /note="Protein ENHANCER OF LHP1 1"
FT /id="PRO_0000455091"
FT REPEAT 15..55
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 60..99
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 102..143
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 144..183
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 192..232
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 236..275
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 277..316
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 347..370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 385..419
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 851..877
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 919..953
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 900..907
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 926..940
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 953 AA; 104853 MW; 411B69CA2A252E34 CRC64;
MKSRSLKLRE AHKVGGSAAF CSILWDHKAE HFVTSSSSDP SISVHDGLST STLPPTILRH
HQDGVTSLAL SNDSTLLASG SIDHCVKLYK FPSGEFQTNI TRFTLPIRVL AFNGSGSLLA
AAGDDEGIKL INTFDGSIVR VLKGHKGPVT GLDFHPNGEL LASIDTTGTV LCWELQNGVV
SFTLKGVAPD TGFNTSIVNI PRWSPDGRTL AVPGLRNDVV MYDRFTGEKL FALRGDHLEA
ICYLTWAPNG KYIATSGLDK QVLLWDVDKK QDIDRHKFEE RICCMSWKPN GNALSVIDAK
GRYGVWESLV PSSMLSPTVG VPDIVPKKRN EILDFDDEVE EEIYRASESL DDAMGDSDDG
ESHHTSRKRL RKKTLIDEDV DDAYEELNDG SSLPSASEYR KKSHRGHREK QGARSGAFKG
ISASTKYKMQ SSFQPGATPP EPGKRTFLCY NMLGCITTIE HEGNSRIETD FHDTGRGPRV
SSMIDIYGFT MASINETGCV FANPCKGEKN MSVLMYRPFR SWASNSEWTM RFEGEEVKVV
ANGSGWVAAV TSLNLLRVFS EGGLQKHILS LDGPVVTAVG CKDHLAVVTH VSDCLPSNEQ
VMEFRVFNIS KMTQELKGRV ALTPGSRLTW IGFSEEGSLS SYDSEGVLRV FTSQYGGSWI
PVFSTSKEKK QEENYWVVGL NTSSLYCIAC KYAEMFPQVT PKPILTILDL SLPLASSDLG
AASLENELIL KQLRLYETQR KVDDMALVGV DTTALEDEAF DLEVSQDKCI LRLISSCCSS
DSFARASELM ELLTLEKSMR AAITLVTKLK LPFLAEKFSS ILEERLLEEA SEAAVTNPAL
NPNGEVVTRV ESKVQNPPAS IQTSENTEAV MKSSATKLSA PTLLKKSKVS EGLKLGKEQT
KKDKSDDAKI KEIKKLNLKN PVNNVNKEDK GQEKEVNQGE ARRSSNPFLK STV
