AGL3_ARATH
ID AGL3_ARATH Reviewed; 258 AA.
AC P29383; O04729; Q41252; Q7XJL0; Q8VZR9;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Agamous-like MADS-box protein AGL3;
DE AltName: Full=Protein SEPALLATA 4 {ECO:0000303|PubMed:15530395};
GN Name=AGL3; Synonyms=SEP4 {ECO:0000303|PubMed:15530395};
GN OrderedLocusNames=At2g03710; ORFNames=F19B11.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=7632923; DOI=10.1007/bf00020401;
RA Huang H., Tudor M., Weiss C.A., Hu Y., Ma H.;
RT "The Arabidopsis MADS-box gene AGL3 is widely expressed and encodes a
RT sequence-specific DNA-binding protein.";
RL Plant Mol. Biol. 28:549-567(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE OF 1-141.
RX PubMed=1672119; DOI=10.1101/gad.5.3.484;
RA Ma H., Yanofsky M.F., Meyerowitz E.M.;
RT "AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic
RT and transcription factor genes.";
RL Genes Dev. 5:484-495(1991).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15530395; DOI=10.1016/j.cub.2004.10.028;
RA Ditta G., Pinyopich A., Robles P., Pelaz S., Yanofsky M.F.;
RT "The SEP4 gene of Arabidopsis thaliana functions in floral organ and
RT meristem identity.";
RL Curr. Biol. 14:1935-1940(2004).
RN [8]
RP INTERACTION WITH TT16/AGL32.
RX PubMed=16080001; DOI=10.1007/s00438-005-0010-y;
RA Kaufmann K., Anfang N., Saedler H., Theissen G.;
RT "Mutant analysis, protein-protein interactions and subcellular localization
RT of the Arabidopsis B sister (ABS) protein.";
RL Mol. Genet. Genomics 274:103-118(2005).
RN [9]
RP FUNCTION, AND SUBUNIT.
RX PubMed=25183521; DOI=10.1093/nar/gku755;
RA Jetha K., Theissen G., Melzer R.;
RT "Arabidopsis SEPALLATA proteins differ in cooperative DNA-binding during
RT the formation of floral quartet-like complexes.";
RL Nucleic Acids Res. 42:10927-10942(2014).
CC -!- FUNCTION: Probable transcription factor that binds specifically to the
CC CArG box DNA sequence 5'-CC (A/T)6 GG-3' (PubMed:7632923,
CC PubMed:25183521). Plays an important role in the determination of
CC flower meristem identity. Involved in the specification of sepal
CC identity. Contributes to the development of petals, stamens and carpels
CC (PubMed:15530395). {ECO:0000269|PubMed:15530395,
CC ECO:0000269|PubMed:25183521, ECO:0000269|PubMed:7632923}.
CC -!- SUBUNIT: Forms homodimers (PubMed:25183521). Interacts with TT16/AGL32
CC (PubMed:16080001). {ECO:0000269|PubMed:16080001,
CC ECO:0000269|PubMed:25183521}.
CC -!- INTERACTION:
CC P29383; Q9FHZ1: SCL23; NbExp=3; IntAct=EBI-622036, EBI-1238460;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=P29383-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P29383-2; Sequence=VSP_008908;
CC Name=3;
CC IsoId=P29383-3; Sequence=VSP_041587;
CC -!- TISSUE SPECIFICITY: Expressed in aerial vegetative organs and flowers,
CC but not in roots (PubMed:7632923). Expressed in flower primordia
CC (PubMed:15530395). {ECO:0000269|PubMed:15530395,
CC ECO:0000269|PubMed:7632923}.
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DR EMBL; U81369; AAB38975.1; -; mRNA.
DR EMBL; AY141229; AAN52793.1; -; mRNA.
DR EMBL; AC006836; AAD20073.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05738.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05739.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05740.1; -; Genomic_DNA.
DR EMBL; AY063894; AAL36250.1; -; mRNA.
DR EMBL; AY096386; AAM20027.1; -; mRNA.
DR PIR; S57793; S57793.
DR RefSeq; NP_178466.1; NM_126418.2. [P29383-1]
DR RefSeq; NP_849930.1; NM_179599.2. [P29383-3]
DR RefSeq; NP_973411.1; NM_201682.3. [P29383-2]
DR AlphaFoldDB; P29383; -.
DR SMR; P29383; -.
DR BioGRID; 299; 20.
DR DIP; DIP-33748N; -.
DR IntAct; P29383; 19.
DR STRING; 3702.AT2G03710.1; -.
DR PaxDb; P29383; -.
DR PRIDE; P29383; -.
DR ProteomicsDB; 244886; -. [P29383-1]
DR EnsemblPlants; AT2G03710.1; AT2G03710.1; AT2G03710. [P29383-1]
DR EnsemblPlants; AT2G03710.2; AT2G03710.2; AT2G03710. [P29383-3]
DR EnsemblPlants; AT2G03710.3; AT2G03710.3; AT2G03710. [P29383-2]
DR GeneID; 814898; -.
DR Gramene; AT2G03710.1; AT2G03710.1; AT2G03710. [P29383-1]
DR Gramene; AT2G03710.2; AT2G03710.2; AT2G03710. [P29383-3]
DR Gramene; AT2G03710.3; AT2G03710.3; AT2G03710. [P29383-2]
DR KEGG; ath:AT2G03710; -.
DR Araport; AT2G03710; -.
DR TAIR; locus:2044259; AT2G03710.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_2_1; -.
DR InParanoid; P29383; -.
DR OMA; NLQHPRF; -.
DR PhylomeDB; P29383; -.
DR PRO; PR:P29383; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P29383; baseline and differential.
DR Genevisible; P29383; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048440; P:carpel development; IMP:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0010076; P:maintenance of floral meristem identity; IMP:TAIR.
DR GO; GO:0048441; P:petal development; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048442; P:sepal development; IMP:TAIR.
DR GO; GO:0048443; P:stamen development; IMP:TAIR.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; DNA-binding;
KW Flowering; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..258
FT /note="Agamous-like MADS-box protein AGL3"
FT /id="PRO_0000199458"
FT DOMAIN 3..57
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 88..178
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT REGION 186..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..211
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 172..258
FT /note="LEDSDAALTQSFWGSSAAEQQQQHQQQQQGMSSYQSNPPIQEAGFFKPLQGN
FT VALQMSSHYNHNPANATNSATTSQNVNGFFPGWMV -> VAIGIFYRKKLKPFVI (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:12837945"
FT /id="VSP_008908"
FT VAR_SEQ 229
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_041587"
FT CONFLICT 58..92
FT /note="Missing (in Ref. 6)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 258 AA; 29573 MW; A642CA7EA58AB38D CRC64;
MGRGKVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEIALLIFSN RGKLYEFCSS
PSGMARTVDK YRKHSYATMD PNQSAKDLQD KYQDYLKLKS RVEILQHSQR HLLGEELSEM
DVNELEHLER QVDASLRQIR STKARSMLDQ LSDLKTKEEM LLETNRDLRR KLEDSDAALT
QSFWGSSAAE QQQQHQQQQQ GMSSYQSNPP IQEAGFFKPL QGNVALQMSS HYNHNPANAT
NSATTSQNVN GFFPGWMV
