ID   ENOX_FRAVE              Reviewed;         322 AA.
AC   O23939;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 2.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=2-methylene-furan-3-one reductase;
DE            EC=1.3.1.105 {ECO:0000250|UniProtKB:Q84V25};
DE   AltName: Full=Enone oxidoreductase;
DE   AltName: Full=Quinone oxidoreductase;
DE            Short=FaQR;
GN   Name=EO; Synonyms=QR;
OS   Fragaria vesca (Woodland strawberry) (Potentilla vesca).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC   Fragaria.
OX   NCBI_TaxID=57918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Nam Y.W., Tichit L., Marty I., Lelievre J.M.;
RT   "Isolation and characterization of cDNAs from genes differentially
RT   expressed during ripening of wild strawberry (Fragaria vesca L.).";
RL   Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=16517758; DOI=10.1105/tpc.105.039784;
RA   Raab T., Lopez-Raez J.A., Klein D., Caballero J.L., Moyano E., Schwab W.,
RA   Munoz-Blanco J.;
RT   "FaQR, required for the biosynthesis of the strawberry flavor compound 4-
RT   hydroxy-2,5-dimethyl-3(2H)-furanone, encodes an enone oxidoreductase.";
RL   Plant Cell 18:1023-1037(2006).
CC   -!- FUNCTION: Enone oxidoreductase involved in the biosynthesis of 4-
CC       hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF or furaneol), the key flavor
CC       compound in strawberries. {ECO:0000250|UniProtKB:Q84V25}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-hydroxy-2,5-dimethyl-furan-3(2H)-one + NADP(+) = 4-hydroxy-
CC         5-methyl-2-methylenefuran-3(2H)-one + H(+) + NADPH;
CC         Xref=Rhea:RHEA:39111, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:76245, ChEBI:CHEBI:76247;
CC         EC=1.3.1.105; Evidence={ECO:0000250|UniProtKB:Q84V25};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q84V25}.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. Quinone oxidoreductase subfamily. {ECO:0000305}.
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DR   EMBL; AJ001445; CAA04767.1; -; mRNA.
DR   SMR; O23939; -.
DR   BRENDA; 1.3.1.105; 2322.
DR   GO; GO:0102978; F:furaneol oxidoreductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   InterPro; IPR013154; ADH_N.
DR   InterPro; IPR044626; AOR-like.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS.
DR   PANTHER; PTHR44573; PTHR44573; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1.
PE   2: Evidence at transcript level;
KW   NAD; NADP; Oxidoreductase.
FT   CHAIN           1..322
FT                   /note="2-methylene-furan-3-one reductase"
FT                   /id="PRO_0000428655"
FT   BINDING         59
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         59
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         174..175
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         197..200
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         215
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         253
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         264..266
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         311..312
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   322 AA;  34174 MW;  47E81314713259F5 CRC64;
     MAAAPSESIP SVNKAWVXSE YGKTSDVLKF DPSVAVPEIK EDQVLIKVVA ASLNPVDFKR
     ALGYFKDTDS PLPTIPGYYV AGVVVKVGSQ VTKFKVGDEV YGDLNETALV NPTRFGSLAE
     YTAADERVLA HKPKNLSFIE AASLPLAIET AHEGLERAEL SAGKSVLVLG GAGGVGTHII
     QLAKHVFGAS KVAATASTKK LDLLRTLGAD LAIDYTKENF EDLPEKFDVV YDAVGETDKA
     VKAVKEGGKV VTIVGPATPP AILFVLTSKG SVLEKLKPYL ESGKVKPVLD PTSPYPFTKV
     VEAFGYLESS RATGKVVVYP IP