AGL5_ARATH
ID AGL5_ARATH Reviewed; 246 AA.
AC P29385; A0JQ83; Q84WG7; Q9SJH3;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 25-MAY-2022, entry version 164.
DE RecName: Full=Agamous-like MADS-box protein AGL5;
DE AltName: Full=Protein SHATTERPROOF 2;
GN Name=AGL5; Synonyms=SHP2; OrderedLocusNames=At2g42830; ORFNames=F7D19.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=1672119; DOI=10.1101/gad.5.3.484;
RA Ma H., Yanofsky M.F., Meyerowitz E.M.;
RT "AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic
RT and transcription factor genes.";
RL Genes Dev. 5:484-495(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 68-246 (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP INTERACTION WITH AGL15 AND AGL16.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [7]
RP FUNCTION.
RX PubMed=27776173; DOI=10.1371/journal.pone.0165075;
RA Ehlers K., Bhide A.S., Tekleyohans D.G., Wittkop B., Snowdon R.J.,
RA Becker A.;
RT "The MADS box genes ABS, SHP1, and SHP2 are essential for the coordination
RT of cell divisions in ovule and seed coat development and for endosperm
RT formation in Arabidopsis thaliana.";
RL PLoS ONE 11:E0165075-E0165075(2016).
CC -!- FUNCTION: Probable transcription factor. Interacts genetically with
CC TT16/AGL32 in a partially antagonistic manner during flower
CC development. Is essential for the coordination of cell divisions in
CC ovule, seed coat development and endosperm formation (PubMed:27776173).
CC {ECO:0000269|PubMed:27776173}.
CC -!- SUBUNIT: Interacts with AGL15 and AGL16. {ECO:0000269|PubMed:15805477}.
CC -!- INTERACTION:
CC P29385; Q9SZJ6: AGL21; NbExp=4; IntAct=EBI-621949, EBI-621986;
CC P29385; P29382: SEP1; NbExp=3; IntAct=EBI-621949, EBI-632935;
CC P29385; Q9FVC1: SVP; NbExp=4; IntAct=EBI-621949, EBI-592058;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P29385-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P29385-2; Sequence=VSP_008909;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M55553; AAA32735.1; -; mRNA.
DR EMBL; AC006931; AAD21741.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10174.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10175.1; -; Genomic_DNA.
DR EMBL; BT029453; ABK59682.1; -; mRNA.
DR EMBL; BT003841; AAO41892.1; -; mRNA.
DR PIR; E39534; E39534.
DR PIR; G84858; G84858.
DR RefSeq; NP_565986.1; NM_129844.5. [P29385-1]
DR RefSeq; NP_850377.1; NM_180046.2. [P29385-2]
DR AlphaFoldDB; P29385; -.
DR SMR; P29385; -.
DR BioGRID; 4220; 18.
DR IntAct; P29385; 19.
DR STRING; 3702.AT2G42830.2; -.
DR PaxDb; P29385; -.
DR PRIDE; P29385; -.
DR ProteomicsDB; 244862; -. [P29385-1]
DR EnsemblPlants; AT2G42830.1; AT2G42830.1; AT2G42830. [P29385-1]
DR EnsemblPlants; AT2G42830.2; AT2G42830.2; AT2G42830. [P29385-2]
DR GeneID; 818883; -.
DR Gramene; AT2G42830.1; AT2G42830.1; AT2G42830. [P29385-1]
DR Gramene; AT2G42830.2; AT2G42830.2; AT2G42830. [P29385-2]
DR KEGG; ath:AT2G42830; -.
DR Araport; AT2G42830; -.
DR TAIR; locus:2052420; AT2G42830.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_0_1; -.
DR InParanoid; P29385; -.
DR OMA; HDEMVAP; -.
DR OrthoDB; 1270226at2759; -.
DR PhylomeDB; P29385; -.
DR PRO; PR:P29385; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P29385; baseline and differential.
DR Genevisible; P29385; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0048440; P:carpel development; IMP:TAIR.
DR GO; GO:0048481; P:plant ovule development; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Flowering;
KW Growth regulation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..246
FT /note="Agamous-like MADS-box protein AGL5"
FT /id="PRO_0000199459"
FT DOMAIN 18..72
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 102..192
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT VAR_SEQ 171
FT /note="R -> RVK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.4"
FT /id="VSP_008909"
SQ SEQUENCE 246 AA; 28157 MW; D4D59316D55606D9 CRC64;
MEGGASNEVA ESSKKIGRGK IEIKRIENTT NRQVTFCKRR NGLLKKAYEL SVLCDAEVAL
VIFSTRGRLY EYANNSVRGT IERYKKACSD AVNPPTITEA NTQYYQQEAS KLRRQIRDIQ
NLNRHILGES LGSLNFKELK NLESRLEKGI SRVRSKKHEM LVAEIEYMQK REIELQNDNM
YLRSKITERT GLQQQESSVI HQGTVYESGV TSSHQSGQYN RNYIAVNLLE PNQNSSNQDQ
PPLQLV
