ID   AGL61_ARATH             Reviewed;         264 AA.
AC   Q4PSU4; Q9SK48;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Agamous-like MADS-box protein AGL61;
DE   AltName: Full=Protein DIANA;
GN   Name=AGL61; Synonyms=DIA; OrderedLocusNames=At2g24840; ORFNames=F27C12.24;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 39-264, FUNCTION, SUBUNIT, SUBCELLULAR
RP   LOCATION, TISSUE SPECIFICITY, AND INTERACTION WITH AGL80.
RX   PubMed=18599653; DOI=10.1104/pp.108.119404;
RA   Steffen J.G., Kang I.-H., Portereiko M.F., Lloyd A., Drews G.N.;
RT   "AGL61 interacts with AGL80 and is required for central cell development in
RT   Arabidopsis.";
RL   Plant Physiol. 148:259-268(2008).
RN   [5]
RP   FUNCTION, INTERACTION WITH AGL80; AGL86; PHE1 AND PHE2, DEVELOPMENTAL
RP   STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=18713950; DOI=10.1105/tpc.108.058958;
RA   Bemer M., Wolters-Arts M., Grossniklaus U., Angenent G.C.;
RT   "The MADS domain protein DIANA acts together with AGAMOUS-LIKE80 to specify
RT   the central cell in Arabidopsis ovules.";
RL   Plant Cell 20:2088-2101(2008).
CC   -!- FUNCTION: Probable transcription factor. Controls central cell
CC       differentiation during female gametophyte development.
CC       {ECO:0000269|PubMed:18599653, ECO:0000269|PubMed:18713950}.
CC   -!- SUBUNIT: Interacts with PHE1/AGL37, PHE2/AGL38, AGL80 and AGL86. Forms
CC       a heterodimer with AGL80. {ECO:0000269|PubMed:18599653,
CC       ECO:0000269|PubMed:18713950}.
CC   -!- INTERACTION:
CC       Q4PSU4; Q9FJK3: AGL80; NbExp=4; IntAct=EBI-1237976, EBI-622424;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251,
CC       ECO:0000269|PubMed:18599653}. Note=The transport to the nucleus is
CC       dependent on dimerization with AGL80.
CC   -!- TISSUE SPECIFICITY: Expressed exclusively in the central cell of the
CC       female gametophyte and in early endosperm.
CC       {ECO:0000269|PubMed:18599653, ECO:0000269|PubMed:18713950}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the final stages of embryo sac
CC       development. {ECO:0000269|PubMed:18713950}.
CC   -!- MISCELLANEOUS: Plants lacking AGL61 show a reduced size of the central
CC       cell and a reduced or absent central cell vacuole. When fertilized, the
CC       central cell fails to give rise to endosperm and the embryo sac
CC       degenerates. However, mutated female gametophytesol are not defective
CC       in pollen tube guidance and the synergid function is not impaired.
CC   -!- MISCELLANEOUS: This protein was called 'DIANA' after the virginal Roman
CC       goddess of the hunt.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD23028.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=ACF37253.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AC006585; AAD23028.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC07635.1; -; Genomic_DNA.
DR   EMBL; DQ056542; AAY78694.1; -; mRNA.
DR   EMBL; EU836691; ACF37253.1; ALT_INIT; mRNA.
DR   PIR; B84641; B84641.
DR   RefSeq; NP_850058.1; NM_179727.5.
DR   AlphaFoldDB; Q4PSU4; -.
DR   SMR; Q4PSU4; -.
DR   BioGRID; 2373; 7.
DR   IntAct; Q4PSU4; 12.
DR   STRING; 3702.AT2G24840.1; -.
DR   PaxDb; Q4PSU4; -.
DR   PRIDE; Q4PSU4; -.
DR   EnsemblPlants; AT2G24840.1; AT2G24840.1; AT2G24840.
DR   GeneID; 817021; -.
DR   Gramene; AT2G24840.1; AT2G24840.1; AT2G24840.
DR   KEGG; ath:AT2G24840; -.
DR   Araport; AT2G24840; -.
DR   TAIR; locus:2047304; AT2G24840.
DR   eggNOG; KOG0014; Eukaryota.
DR   HOGENOM; CLU_053053_5_3_1; -.
DR   InParanoid; Q4PSU4; -.
DR   OMA; RQCWWEA; -.
DR   OrthoDB; 1516449at2759; -.
DR   PhylomeDB; Q4PSU4; -.
DR   PRO; PR:Q4PSU4; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q4PSU4; baseline and differential.
DR   Genevisible; Q4PSU4; AT.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0043078; C:polar nucleus; IDA:TAIR.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0009559; P:embryo sac central cell differentiation; IMP:TAIR.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00265; MADS_MEF2_like; 1.
DR   Gene3D; 3.40.1810.10; -; 1.
DR   InterPro; IPR033896; MADS_MEF2-like.
DR   InterPro; IPR002100; TF_MADSbox.
DR   InterPro; IPR036879; TF_MADSbox_sf.
DR   Pfam; PF00319; SRF-TF; 1.
DR   PRINTS; PR00404; MADSDOMAIN.
DR   SMART; SM00432; MADS; 1.
DR   SUPFAM; SSF55455; SSF55455; 1.
DR   PROSITE; PS50066; MADS_BOX_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..264
FT                   /note="Agamous-like MADS-box protein AGL61"
FT                   /id="PRO_0000363649"
FT   DOMAIN          62..122
FT                   /note="MADS-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
SQ   SEQUENCE   264 AA;  30264 MW;  517BACA6BF7B4CDC CRC64;
     MYVTKYKNKL PTLQVDLIIM PHQTQACIYK QTLSLHQIFQ QRKATKTLHT KKTMMSKKKE
     SIGRQKIPMV KIKKESHRQV TFSKRRAGLF KKASELCTLC GAEIGIIVFS PAKKPFSFGH
     PSVESVLDRY VSRNNMSLAQ SQQLQGSPAA SCELNMQLTH ILSEVEEEKK KGQAMEEMRK
     ESVRRSMINW WEKPVEEMNM VQLQEMKYAL EELRKTVVTN MASFNEAKDD VFGFLDNKVT
     VPPYVNMPSG PSNIYNFANG NGCF