AGL65_ARATH
ID AGL65_ARATH Reviewed; 389 AA.
AC Q7X9I0; Q76K80; Q9M9U6;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Agamous-like MADS-box protein AGL65 {ECO:0000305};
GN Name=AGL65 {ECO:0000303|PubMed:12837945};
GN OrderedLocusNames=At1g18750 {ECO:0000312|Araport:AT1G18750};
GN ORFNames=F6A14.14 {ECO:0000312|EMBL:AAF27105.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 23-380 (ISOFORM 2), AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [5]
RP FUNCTION, AND INTERACTION WITH AGL104.
RX PubMed=19211705; DOI=10.1104/pp.109.135806;
RA Adamczyk B.J., Fernandez D.E.;
RT "MIKC* MADS domain heterodimers are required for pollen maturation and tube
RT growth in Arabidopsis.";
RL Plant Physiol. 149:1713-1723(2009).
CC -!- FUNCTION: Probable transcription factor that forms a heterodimer with
CC the MADS-box protein AGL104 and is involved in the regulation of pollen
CC maturation at the late stages of pollen development and pollen tube
CC growth. {ECO:0000269|PubMed:19211705}.
CC -!- SUBUNIT: Forms a heterodimer with AGL104.
CC {ECO:0000269|PubMed:19211705}.
CC -!- INTERACTION:
CC Q7X9I0; Q9LM46: AGL104; NbExp=3; IntAct=EBI-622332, EBI-15194999;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7X9I0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7X9I0-2; Sequence=VSP_057857;
CC -!- TISSUE SPECIFICITY: Expressed in pollen. {ECO:0000269|PubMed:12949148}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF27105.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY143172; AAN37407.1; -; mRNA.
DR EMBL; AC011809; AAF27105.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29758.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59116.1; -; Genomic_DNA.
DR EMBL; AB094116; BAC99091.1; -; mRNA.
DR PIR; D86321; D86321.
DR RefSeq; NP_001321506.1; NM_001332367.1. [Q7X9I0-2]
DR RefSeq; NP_173310.2; NM_101733.6. [Q7X9I0-1]
DR AlphaFoldDB; Q7X9I0; -.
DR SMR; Q7X9I0; -.
DR IntAct; Q7X9I0; 8.
DR STRING; 3702.AT1G18750.1; -.
DR PaxDb; Q7X9I0; -.
DR PRIDE; Q7X9I0; -.
DR ProteomicsDB; 244774; -. [Q7X9I0-1]
DR EnsemblPlants; AT1G18750.1; AT1G18750.1; AT1G18750. [Q7X9I0-1]
DR EnsemblPlants; AT1G18750.4; AT1G18750.4; AT1G18750. [Q7X9I0-2]
DR GeneID; 838457; -.
DR Gramene; AT1G18750.1; AT1G18750.1; AT1G18750. [Q7X9I0-1]
DR Gramene; AT1G18750.4; AT1G18750.4; AT1G18750. [Q7X9I0-2]
DR KEGG; ath:AT1G18750; -.
DR Araport; AT1G18750; -.
DR TAIR; locus:2034949; AT1G18750.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_034796_0_0_1; -.
DR InParanoid; Q7X9I0; -.
DR PhylomeDB; Q7X9I0; -.
DR PRO; PR:Q7X9I0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q7X9I0; baseline and differential.
DR Genevisible; Q7X9I0; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0010152; P:pollen maturation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0080092; P:regulation of pollen tube growth; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00266; MADS_SRF_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033897; MADS_SRF-like.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..389
FT /note="Agamous-like MADS-box protein AGL65"
FT /id="PRO_0000433967"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT REGION 310..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 77..131
FT /evidence="ECO:0000255"
FT COILED 293..325
FT /evidence="ECO:0000255"
FT COMPBIAS 310..331
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 378..389
FT /note="VCVFTPPLELSR -> QPN (in isoform 2)"
FT /id="VSP_057857"
SQ SEQUENCE 389 AA; 44877 MW; C0BC0BB0B528771D CRC64;
MGRVKLKIKR LESTSNRQVT YTKRKNGILK KAKELSILCD IDIVLLMFSP TGRATAFHGE
HSCIEEVISK FAQLTPQERT KRKLESLEAL KKTFKKLDHD VNIHDFLGAR NQTIEGLSNQ
VAIYQAQLME CHRRLSCWTN IDRIENTEHL DLLEESLRKS IERIQIHKEH YRKNQLLPIE
CATTQFHSGI QLPMAMGGNS SMQEAHSMSW LPDNDHQQTI LPGDSSFLPH REMDGSIPVY
SSCFFESTKP EDQICSNPGQ QFEQLEQQGN GCLGLQQLGE EYSYPTPFGT TLGMEEDQEK
KIKSEMELNN LQQQQQQQQQ QQQQDPSMYD PMANNNGGCF QIPHDQSMFV NDHHHHHHHH
HQNWVPDSMF GQTSYNQVCV FTPPLELSR
